公开(公告)号：US20210024868A1

公开(公告)日：2021-01-28

申请号：US17039218

申请日：2020-09-30

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral

IPC: C12M1/12 ,  C12M1/00 ,  C12N5/00 ,  C12M3/00 ,  C12M1/34 ,  C12N5/071

Abstract: A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

公开(公告)号：US20200255783A1

公开(公告)日：2020-08-13

申请号：US16781807

申请日：2020-02-04

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Lori E. Romeo

IPC: C12M1/12 ,  C12M1/00

Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

公开(公告)号：US20200248121A1

公开(公告)日：2020-08-06

申请号：US16781723

申请日：2020-02-04

Applicant: CORNING INCORPORATED

Inventor： Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Lori E Romeo

IPC: C12M1/12 ,  C12M1/00 ,  C12N5/00

Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

公开(公告)号：US20190322969A1

公开(公告)日：2019-10-24

申请号：US16447453

申请日：2019-06-20

Applicant: Corning Incorporated

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Gregory Roger Martin ,  Kaitlyn Mary Matias ,  Michael Kurt Schaefer ,  Allison Jean Tanner

IPC: C12M1/32 ,  C12M1/04 ,  C12M3/06 ,  C12M1/26 ,  C12M1/00

Abstract: The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.

公开(公告)号：US10203320B2

公开(公告)日：2019-02-12

申请号：US14437275

申请日：2013-11-15

Applicant: Corning Incorporated

Inventor： Oberon Denaci Deichmann ,  Ye Fang ,  Ann MeeJin Ferrie ,  David Henry ,  Haibei Hu ,  Corinne Walerack ,  Qi Wu

IPC: G01N33/50 ,  G01N15/14 ,  G01N21/31 ,  G01N21/77 ,  G01N15/00

Abstract: A label-free resonant waveguide grating biosensor imager system for measuring beat patterns and dynamic mass redistribution (DMR) signals of cultured cardiomyocytes in the absence and presence of a drug molecule. The disclosure also provides a method using the imager system for analyzing the beat patterns and the DMR signals of the cardiomyocytes to assess drug-induced cardiotoxicity.