公开(公告)号：US10961540B2

公开(公告)日：2021-03-30

申请号：US16698077

申请日：2019-11-27

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/63 ,  C12N15/79

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20190327928A1

公开(公告)日：2019-10-31

申请号：US16460660

申请日：2019-07-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Vipula Shukla ,  Manju Gupta ,  Fyodor Urnov ,  Dmitry Y. Guschin ,  Michiel Jan De Both ,  Paul Bundock ,  Lakshmi Sastry-Dent

IPC: A01H6/82 ,  C12N15/82

Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

公开(公告)号：US10273494B2

公开(公告)日：2019-04-30

申请号：US15484581

申请日：2017-04-11

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C12N15/82 ,  C12N15/00

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US20180363067A1

公开(公告)日：2018-12-20

申请号：US16011908

申请日：2018-06-19

Applicant: Pioneer Hi-Bred International, Inc. ,  Dow Agrosciences LLC ,  E. I. Du Pont De Nemours and Company

Inventor： Eric Barbour ,  James Wayne Bing ,  Guy Cardineau ,  Robert F. Cressman, JR. ,  Manju Gupta ,  Mary E. Hartnett Locke ,  David Hondred ,  Joseph W. Keaschall ,  Michael G. Koziel ,  Terry EuClaire Meyer ,  Daniel Moellenbeck ,  Kenneth Edwin Narva ,  Wilas Nirunsuksiri ,  Steven W. Ritchie ,  Marjorie L. Rudert ,  Craig D. Sanders ,  Aihua Shao ,  Steven Jeffrey Stelman ,  David S. Stucker ,  Laura Ann Tagliani ,  William M. Van Zante

IPC: C12Q1/6895 ,  C12N15/82

Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize TC1507 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

公开(公告)号：US20180223297A1

公开(公告)日：2018-08-09

申请号：US15943106

申请日：2018-04-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C07K14/47 ,  C12N9/22 ,  C12N15/90 ,  C12N15/66 ,  C12N15/10

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US10036028B2

公开(公告)日：2018-07-31

申请号：US14587757

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v1.

公开(公告)号：US10030246B2

公开(公告)日：2018-07-24

申请号：US14587735

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.

公开(公告)号：US09963711B2

公开(公告)日：2018-05-08

申请号：US14019244

申请日：2013-09-05

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N15/66 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  C07K14/47

CPC classification number: C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US09914930B2

公开(公告)日：2018-03-13

申请号：US14019211

申请日：2013-09-05

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/79 ,  C12N15/63

CPC classification number: C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US09885054B2

公开(公告)日：2018-02-06

申请号：US15618986

申请日：2017-06-09

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/10 ,  C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.