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1.发明申请FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 审中-公开FAD2性能位置和相应目标位点特异性结合蛋白质可诱导目标突变公开(公告)号：US20170044559A1
公开(公告)日：2017-02-16
申请号：US15292774
申请日：2016-10-13
发明人： W. Michael Ainley , Steven R. Webb , Jayakumar P. Samuel , Dmitry Y. Guschin , Jeffrey C. Miller , Lei Zhang
CPC分类号： C12N15/8216 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N15/102 , C12N15/66 , C12N15/822 , C12N15/8247 , C12N15/902 , C12Y301/00
摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
摘要翻译： FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。
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2.发明申请METHODS AND COMPOSITIONS FOR INTEGRATION OF AN EXOGENOUS SEQUENCE WITHIN THE GENOME OF PLANTS 审中-公开在植物基因组中整合外源序列的方法和组合公开(公告)号：US20140304853A1
公开(公告)日：2014-10-09
申请号：US14243553
申请日：2014-04-02
发明人： W. Michael Ainley , Dmitry Y. Guschin , Matthew Hayden , Daniel Isenegger , John Mason , Jeffrey C. Miller , Joseph F. Petolino , Yidong Ran , Tim Sawbridge , German Spangenberg , Steven R. Webb
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8274 , C12N15/8278 , C12N15/90 , C12N2800/80
摘要： Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.
摘要翻译： 本文公开了用于在植物中平行或顺序转基因堆叠以产生具有选定表型的植物的方法和组合物。
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3.发明申请FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 有权FAD2性能位置和相应目标位点特异性结合蛋白质可诱导目标突变公开(公告)号：US20140090116A1
公开(公告)日：2014-03-27
申请号：US14019293
申请日：2013-09-05
发明人： William Michael Ainley , Steven R. Webb , Pon Samuel , Dmitry Y. Guschin , Jeffrey C. Miller , Lei Zhang
IPC分类号： C12N15/66
CPC分类号： C12N15/8216 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N15/102 , C12N15/66 , C12N15/822 , C12N15/8247 , C12N15/902 , C12Y301/00
摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
摘要翻译： FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。
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公开(公告)号：US10358684B2
公开(公告)日：2019-07-23
申请号：US15379152
申请日：2016-12-14
发明人： Vipula Shukla , Manju Gupta , Fyodor Urnov , Dmitry Y. Guschin , Michiel Jan De Both , Paul Bundock , Lakshmi Sastry-Dent
IPC分类号： C12N15/82
摘要： Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.
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5.发明授权FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks 有权FAD2表达位点和相应的靶位点特异性结合蛋白能够诱导靶向性突变公开(公告)号：US09493779B2
公开(公告)日：2016-11-15
申请号：US14019293
申请日：2013-09-05
发明人： William Michael Ainley , Steven R. Webb , Pon Samuel , Dmitry Y. Guschin , Jeffrey C. Miller , Lei Zhang
CPC分类号： C12N15/8216 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N15/102 , C12N15/66 , C12N15/822 , C12N15/8247 , C12N15/902 , C12Y301/00
摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
摘要翻译： FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。
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6.发明申请FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 有权FAD3性能位置和相应目标位点特异性结合蛋白质可诱导目标突变公开(公告)号：US20150067921A1
公开(公告)日：2015-03-05
申请号：US14019211
申请日：2013-09-05
发明人： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , William Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
CPC分类号： C12N15/79 , C12N9/22 , C12N15/63 , C12N15/8241 , C12N15/8247
摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
摘要翻译： 通过以位点方式切割细胞中FAD3基因的位置，在FAD3基因座中进行基因编辑或基因堆积的方法，以在FAD3基因中产生断裂，然后连接到断裂中，与核酸分子缔合 公开了一种或多种感兴趣的特征。
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公开(公告)号：US09963711B2
公开(公告)日：2018-05-08
申请号：US14019244
申请日：2013-09-05
发明人： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , William Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
CPC分类号： C12N15/8216 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N15/102 , C12N15/66 , C12N15/822 , C12N15/8247 , C12N15/902 , C12Y301/00
摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
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公开(公告)号：US09914930B2
公开(公告)日：2018-03-13
申请号：US14019211
申请日：2013-09-05
发明人： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , William Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
CPC分类号： C12N15/79 , C12N9/22 , C12N15/63 , C12N15/8241 , C12N15/8247
摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
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公开(公告)号：US20170088825A1
公开(公告)日：2017-03-30
申请号：US15379152
申请日：2016-12-14
发明人： Vipula Shukla , Manju Gupta , Fyodor Urnov , Dmitry Y. Guschin , Michiel Jan De Both , Paul Bundock , Lakshmi Sastry-Dent
CPC分类号： C12Y101/01037 , C12N15/8213 , C12N15/8241 , C12N15/8243 , C12N15/8261 , Y02A40/146
摘要： Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.
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10.发明申请FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 有权FAD2性能位置和相应目标位点特异性结合蛋白质可诱导目标突变公开(公告)号：US20140090112A1
公开(公告)日：2014-03-27
申请号：US14019244
申请日：2013-09-05
发明人： Noel Cogan , John Forster , Matthew Hayden , Tim Sawbridge , German Spangenberg , Steven R. Webb , Manju Gupta , William Michael Ainley , Matthew J. Henry , Jeffrey C. Miller , Dmitry Y. Guschin
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N15/102 , C12N15/66 , C12N15/822 , C12N15/8247 , C12N15/902 , C12Y301/00
摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
摘要翻译： FAD2基因座中基因编辑或基因堆积的方法，以位点方式，以细胞中的FAD2基因的位置切割，在FAD2基因中产生断裂，然后连接到断裂中，与核酸分子缔合 公开了一种或多种感兴趣的特征。
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