公开(公告)号：US20070061917A1

公开(公告)日：2007-03-15

申请号：US11508045

申请日：2006-08-22

申请人： Billy McCutchen ,  Christine Hazel ,  Donglong Liu ,  Albert Lu ,  Wayne Mehre ,  Paul Olson ,  James Wong

发明人： Billy McCutchen ,  Christine Hazel ,  Donglong Liu ,  Albert Lu ,  Wayne Mehre ,  Paul Olson ,  James Wong

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  C12N15/87

CPC分类号： C12N15/8274 ,  C12N9/1092 ,  C12N9/88 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8216 ,  C12N15/8271 ,  C12N15/8275 ,  C12N15/8278 ,  C12N15/8286 ,  Y02A40/162

摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

摘要翻译： 提供了表达感兴趣的多核苷酸的方法和组合物。 组合物包含SEQ ID NO：1，10，15或16所示的增强子结构域及其活性变体和片段。 还提供了包含至少一个转录增强子序列的DNA构建体，所述转录增强子序列包含可操作地连接到异源启动子的SEQ ID NO：1,0,15或16所示的核苷酸序列或其活性变体或片段。 这样的嵌合转录调控区域可以可操作地连接任何感兴趣的多核苷酸。 还提供了包含DNA构建体的细胞，植物，植物部分和种质。 还提供了使用嵌合转录调节区的方法。 在具体实施方案中，提供了表达目标多核苷酸的方法，包括例如赋予除草剂耐受性的序列，以及选择具有DNA构建体的细胞的方法。

公开(公告)号：US07146709B2

公开(公告)日：2006-12-12

申请号：US10736650

申请日：2003-12-16

申请人： James Wong

发明人： James Wong

IPC分类号： H01L39/24

CPC分类号： B21C37/047 ,  C23C2/02 ,  C23C2/08 ,  C23C2/28 ,  H01L39/2409 ,  H01L39/2416 ,  H02G15/34 ,  Y02E40/648 ,  Y10T29/417 ,  Y10T29/49014 ,  Y10T29/49789

摘要： A superconducting material useful for forming electrolytic devices is made by establishing multiple niobium or tantalum components in a primary billet of a ductile material; working the primary billet through a series of reduction steps to form the niobium or tantalum components into elongated elements; cutting and restacking the resulting elongated elements with a porous confining layer to form a secondary billet, working the secondary billet through a series of reduction steps including twisting and final rolling to thin ribbon cross-sections with greater than 5:1 Aspect Ratios; cutting the resulting elongated billet into sections; and leaching the core and sheath at least in part.

摘要翻译： 用于形成电解器件的超导材料是通过在延性材料的原始坯料中建立多个铌或钽成分制成的; 通过一系列还原步骤来处理原坯，以将铌或钽组分形成为细长元件; 用多孔限制层切割和重新排列所得到的细长元件以形成次级坯料，通过一系列减少步骤（包括扭转和最后轧制）至大于5:1的纵横比的薄带横截面来加工第二坯料; 将生成的细长坯料切割成段; 并至少部分浸出芯鞘。

公开(公告)号：US20060021096A1

公开(公告)日：2006-01-26

申请号：US11224624

申请日：2005-09-12

申请人： Andre Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

发明人： Andre Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

IPC分类号： A01H5/00 ,  A01H1/00 ,  C07K14/415

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.

摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。 特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。 本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。 还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

公开(公告)号：US20050164940A1

公开(公告)日：2005-07-28

申请号：US11092430

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A61K38/16 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US20050164939A1

公开(公告)日：2005-07-28

申请号：US11092429

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A61K38/16 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US06918172B2

公开(公告)日：2005-07-19

申请号：US10037935

申请日：2002-01-02

申请人： James Wong

发明人： James Wong

IPC分类号： C23C2/02 ,  C23C2/08 ,  C23C2/28 ,  H01L39/24

CPC分类号： C23C2/28 ,  C23C2/02 ,  C23C2/08 ,  H01L39/2409 ,  Y10T29/49014

摘要： A niobium-based superconductor is manufactured by establishing multiple niobium components in a billet of a ductile metal, working the composite billet through a series of reduction steps to form the niobium components into elongated elements, each niobium element having a thickness on the order of 1 to 25 microns, surrounding the billet prior to the last reduction step with a porous confining layer of an acid resistant metal, immersing the confined billet in an acid or a high temperature liquid metal to remove the ductile metal from between the niobium elements while the niobium elements remain confined by said porous layer, exposing the confined mass of niobium elements to a material capable of reacting with Nb to form a superconductor.

摘要翻译： 铌基超导体通过在延性金属的坯料中建立多个铌组分来制造，通过一系列还原步骤来加工复合坯料，以将铌组分形成为细长元件，每个铌元素的厚度大约为1 至25微米，在最后的还原步骤之前，用耐酸金属的多孔限制层围绕坯料，将受限制的坯料浸入酸或高温液态金属中，以从铌元素之间除去延性金属，而铌 元素仍然被所述多孔层限制，将约束的铌元素暴露于能够与Nb反应的材料以形成超导体。

公开(公告)号：US5930719A

公开(公告)日：1999-07-27

申请号：US715938

申请日：1996-09-19

申请人： Daniel Babitch ,  Andrew G. Varadi ,  James Wong

发明人： Daniel Babitch ,  Andrew G. Varadi ,  James Wong

IPC分类号： H04M1/65 ,  H04M1/725 ,  H04B1/38 ,  H04M11/00

CPC分类号： H04M1/72513 ,  H04M1/6505 ,  H04M1/725 ,  H04M1/72527

摘要： A cordless system comprises a laptop computer with a modem connected to a modem data port on a cordless telephone handset. A direct sequence spread spectrum RF link is established between the cordless telephone handset and a base station. Both the telephone network and a desktop computer with a modem are connected to the base station. When the cordless telephone handset detects that the laptop computer wants to go off-hook, a selection signal is also sent by the cordless telephone handset to the base station to indicate whether the call is to be directed to the telephone network or the desktop computer. When the base station detects that the desktop computer wants to go off-hook, a selection signal is also sent by the desktop computer to the base station to indicate whether the call is to be directed to the telephone network or over the RF link to the laptop computer. Incoming calls from the telephone network are directed to the desktop computer by the base station with a ring indication going to the cordless telephone handset. If the laptop computer answers the ringing, the cordless telephone handset indicates an off-hook condition to the base station and the base station routes the incoming call over the RF link to the cordless telephone handset and the laptop computer.

摘要翻译： 无线系统包括具有连接到无绳电话手机上的调制解调器数据端口的调制解调器的膝上型计算机。 在无绳电话手机和基站之间建立直接序列扩频RF链路。 电话网络和具有调制解调器的台式计算机都连接到基站。 当无绳电话听筒检测到膝上型计算机想要摘机时，无线电话手机也将选择信号发送到基站，以指示呼叫是否被引导到电话网络或台式计算机。 当基站检测到台式计算机想要摘机时，桌面计算机还向基站发送选择信号，以指示该呼叫是要被引导到电话网络还是通过RF链路到达 笔记本电脑。 来自电话网络的来电由基站指向桌面计算机，其中指示进入无绳电话手机。 如果膝上型计算机应答振铃，则无绳电话手机向基站指示摘机状态，并且基站通过RF链路将呼入路由到无绳电话听筒和笔记本电脑。

公开(公告)号：US5404813A

公开(公告)日：1995-04-11

申请号：US431166

申请日：1989-10-23

申请人： James Wong

发明人： James Wong

IPC分类号： C06B45/00

CPC分类号： C06B45/00 ,  Y10S149/114

摘要： The present invention relates to metal filaments for use as fuel additives for rocket propellants, explosives, and other pyrotechnic devices. Preferred filaments are those such as zirconium, niobium and titanium (and alloys thereof) which have very high heat of combustion.

摘要翻译： 本发明涉及用作火箭推进剂，爆炸物和其它烟火装置的燃料添加剂的金属丝。 优选的长丝是具有非常高的燃烧热的锆，铌和钛（及其合金）。

公开(公告)号：US5388518A

公开(公告)日：1995-02-14

申请号：US269884

申请日：1988-11-10

申请人： James Wong

发明人： James Wong

IPC分类号： C06B45/00 ,  C06D5/06 ,  C06B33/00

CPC分类号： C06B45/00 ,  Y10S149/114

摘要： The present invention relates to metal filaments for use as fuel additives for rocket propellants, explosives, and other pyrotechnic devices. Preferred filaments are those such as zirconium, niobium and titanium (and alloys thereof) which have very high heat of combustion.

公开(公告)号：US5160550A

公开(公告)日：1992-11-03

申请号：US560163

申请日：1990-07-31

申请人： James Wong ,  Mark K. Rudziak

发明人： James Wong ,  Mark K. Rudziak

IPC分类号： B21C23/22 ,  B21C37/04 ,  C22F1/18 ,  H01J7/18 ,  H01L39/24

CPC分类号： H01L39/2406 ,  B21C23/22 ,  B21C33/002 ,  B21C37/042 ,  B21C37/045 ,  C22F1/18 ,  C22F1/183 ,  C22F1/186 ,  H01J7/183 ,  Y10S428/93 ,  Y10S505/814 ,  Y10S505/918 ,  Y10S505/921 ,  Y10T29/49014 ,  Y10T428/12812

摘要： A method for producing a superconductor includes the steps of combining a plurality of metal bodies to form a composite structure, metal bodies being selected from transition metals such as niobium, tantalum, titanium, zirconium, hafnium and vanadium, alternate bodies being formed of different transition metals to form triplets. The transition metals are reacted to form a ductile superconducting ternary alloy at the interfaces of these triplets of metals. The extent of the reaction is limited so as to maintain areas of pure or nearly pure transition metal along with the superconducting ternary alloy in at least one of each triplet of transition metals.

摘要翻译： 制造超导体的方法包括以下步骤：将多个金属体组合以形成复合结构，金属体选自铌，钽，钛，锆，铪和钒等过渡金属，由不同转变形成的交替体 金属形成三联体。 过渡金属在金属三元体的界面处反应形成延性超导三元合金。 反应的程度受到限制，以便在过渡金属的每个三元组中的至少一个中保持纯或近纯过渡金属与超导三元合金的区域。