公开(公告)号：US07253343B2

公开(公告)日：2007-08-07

申请号：US10926819

申请日：2004-08-26

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

IPC分类号： A01N5/00 ,  A01N5/10 ,  C12N15/32 ,  C12N15/82 ,  C12P21/04

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体 。

公开(公告)号：US5770696A

公开(公告)日：1998-06-23

申请号：US471033

申请日：1995-06-06

申请人： Gregory W. Warren ,  Michael G. Koziel ,  Martha A. Mullins ,  Gordon J. Nye ,  Brian Carr ,  Nalini M. Desai ,  Kristy Kostichka ,  Nicholas B. Duck ,  Juan J. Estruch

发明人： Gregory W. Warren ,  Michael G. Koziel ,  Martha A. Mullins ,  Gordon J. Nye ,  Brian Carr ,  Nalini M. Desai ,  Kristy Kostichka ,  Nicholas B. Duck ,  Juan J. Estruch

IPC分类号： A01H5/00 ,  A01N63/00 ,  A01N63/02 ,  C07K20060101 ,  C07K14/32 ,  C07K14/325 ,  C07K14/415 ,  C07K14/435 ,  C07K16/00 ,  C12N1/11 ,  C12N1/12 ,  C12N1/13 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N15/09 ,  C12N15/11 ,  C12N15/31 ,  C12N15/32 ,  C12N15/62 ,  C12N15/82 ,  C12N15/84 ,  C12P21/02 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/07 ,  C12R1/085 ,  C12R1/19 ,  C12R1/91 ,  G01N33/00

CPC分类号： A01N63/02 ,  A01N63/00 ,  C07K14/32 ,  C07K14/325 ,  C07K14/415 ,  C07K14/43563 ,  C12N15/8285 ,  C12N15/8286 ,  C12R1/07 ,  C12R1/075 ,  C07K2319/00 ,  C07K2319/02 ,  Y10S530/825

摘要： The present invention is drawn to pesticidal strains and proteins. Bacillus strains which are capable of producing pesticidal proteins and auxiliary proteins during vegetative growth are provided. "The auxiliary proteins enhance the insecticidal activity of pesticidal proteins." Also provided are the purified proteins, nucleotide sequences encoding the proteins and methods for using the strains, proteins and genes for controlling pests.

摘要翻译： 本发明涉及杀虫菌株和蛋白质。 提供了能够在营养生长期间产生杀虫蛋白和辅助蛋白质的芽孢杆菌菌株。 “辅助蛋白提高杀虫蛋白的杀虫活性。 还提供了纯化的蛋白质，编码蛋白质的核苷酸序列和使用菌株，蛋白质和基因来控制害虫的方法。

公开(公告)号：US08314292B2

公开(公告)日：2012-11-20

申请号：US12166124

申请日：2008-07-01

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Tracy Hargiss ,  Nicholas B. Duck

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Tracy Hargiss ,  Nicholas B. Duck

IPC分类号： C12N15/82

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。

公开(公告)号：US08236757B2

公开(公告)日：2012-08-07

申请号：US12691115

申请日：2010-01-21

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck

IPC分类号： A01N37/18 ,  C07K14/325

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,11和13所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,10和12所示的核苷酸序列，作为 以及其变体和片段。

公开(公告)号：US08188036B2

公开(公告)日：2012-05-29

申请号：US12472997

申请日：2009-05-27

申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

IPC分类号： A01N37/18

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

摘要翻译： 本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。 这些组合物可用于防治害虫，特别是植物害虫的方法。

公开(公告)号：US20110136200A1

公开(公告)日：2011-06-09

申请号：US13027666

申请日：2011-02-15

申请人： Nicholas B. Duck ,  Todd K. Hinson ,  Vadim Beilinson ,  Laura Cooper Schouten ,  Daniel John Tomso

发明人： Nicholas B. Duck ,  Todd K. Hinson ,  Vadim Beilinson ,  Laura Cooper Schouten ,  Daniel John Tomso

IPC分类号： C12N9/00

CPC分类号： C12N15/8277 ,  C12N9/93

摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.

摘要翻译： 提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。 提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。 另外，包括对应于多核苷酸的多肽。 特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

公开(公告)号：US07803925B2

公开(公告)日：2010-09-28

申请号：US12166239

申请日：2008-07-01

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Tracy Hargiss ,  Nicholas B. Duck ,  Theodore W. Kahn

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Tracy Hargiss ,  Nicholas B. Duck ,  Theodore W. Kahn

IPC分类号： C07H21/04 ,  C12P21/02 ,  A01N33/00 ,  A61K31/7052

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

公开(公告)号：US20090291896A1

公开(公告)日：2009-11-26

申请号：US12472997

申请日：2009-05-27

申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

IPC分类号： A01N37/18 ,  C07K7/08 ,  C07K14/00 ,  A01P17/00

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。 这些组合物可用于防治害虫，特别是植物害虫的方法。

公开(公告)号：US07622572B2

公开(公告)日：2009-11-24

申请号：US11416261

申请日：2006-05-02

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Tracy Hargiss ,  Nicholas B. Duck

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Tracy Hargiss ,  Nicholas B. Duck

IPC分类号： C07H21/04 ,  C12P21/02 ,  A01N43/04 ,  A61K31/7052

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。

公开(公告)号：US07605304B2

公开(公告)日：2009-10-20

申请号：US10032717

申请日：2001-10-23

申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

摘要翻译： 本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。