公开(公告)号：US20210115407A1

公开(公告)日：2021-04-22

申请号：US16604596

申请日：2018-04-12

申请人： The Broad Institute, Inc. ,  The General Hospital Corporation ,  Massachusetts Institute of Technology

发明人： Jayaraj Rajagopal ,  Aviv Regev ,  Moshe Biton ,  Adam Haber ,  Daniel Montoro ,  Orit Rozenblatt-Rosen

IPC分类号： C12N5/071 ,  A61K35/42 ,  A61P11/00 ,  G01N33/50

摘要： Provided herein are methods and compositions for identifying ionocytes from respiratory epithelial cells, and uses of such cells for treatment of inflammatory lung disease. Also provided herein are methods and compositions for modulating respiratory tract epithelial cell proliferation, differentiation, maintenance, and/or function.

公开(公告)号：US20180255751A1

公开(公告)日：2018-09-13

申请号：US15468652

申请日：2017-03-24

申请人： The Broad Institute Inc. ,  Massachusetts Institute of Technology ,  The General Hospital Corporation

发明人： Aviv Regev ,  Oren PARNAS ,  Marko JOVANOVIC ,  Nir HACOHEN ,  Thomas EISENHAURE

IPC分类号： A01K67/027 ,  C12N15/10 ,  C12N15/85 ,  C12N15/90 ,  C12N9/22 ,  C12N7/04 ,  A61K48/00

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2207/12 ,  A01K2217/052 ,  A01K2217/077 ,  A01K2227/105 ,  A01K2267/0312 ,  A01K2267/0331 ,  A01K2267/0362 ,  A61K48/005 ,  C07K2319/09 ,  C12N7/04 ,  C12N9/22 ,  C12N15/1082 ,  C12N15/111 ,  C12N15/113 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3513 ,  C12N2310/531 ,  C12N2320/12 ,  C12N2330/31 ,  C12N2330/51 ,  C12N2740/15043 ,  C12N2750/14143

摘要： The invention involves a method for modulating leukocyte activity, comprising delivering to a leukocyte a vector containing nucleic acid molecule(s), whereby the leukocyte contains Cas9 and the vector expresses one or more RNAs to guide the Cas9 to introduce mutations in one or more target genetic loci in the leukocyte, thereby modulating expression of one or more genes expressed in the leukocyte. The invention also involves identifying genes associated with leukocyte responses and experimental modeling of aberrant leukocyte activation and diseases associated with leukocytes by introducing mutations into leukocytes. The invention comprehends testing putative treatments with such models, e.g., testing putative chemical compounds that may be pharmaceutically relevant for treatment or gene therapy that may be relevant for treatment, or combinations thereof. The invention allows for the study of genetic diseases and putative treatments to better understand and alleviate leukocyte associated diseases.

公开(公告)号：US11963966B2

公开(公告)日：2024-04-23

申请号：US16499393

申请日：2018-03-30

申请人： Dana-Farber Cancer Institute, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Levi Garraway ,  Benjamin Izar ,  Aviv Regev ,  Orit Rozenblatt-Rosen ,  Asaf Rotem ,  Elizabeth Stover ,  Itay Tirosh

IPC分类号： A61K31/575 ,  A61K9/00 ,  A61K33/243 ,  A61K35/17 ,  A61K39/395

CPC分类号： A61K31/575 ,  A61K9/0019 ,  A61K33/243 ,  A61K35/17 ,  A61K39/39533

摘要： Described herein are compositions and methods of using single-cell RNA-sequencing to identify treatment resistance in patients with ovarian cancer. Also, described herein are compositions and methods for treatment targeting resistance in patients with ovarian cancer.

公开(公告)号：US20220119871A1

公开(公告)日：2022-04-21

申请号：US17426453

申请日：2020-01-28

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Sanja Vickovic

IPC分类号： C12Q1/6841 ,  C12N15/10 ,  C12N9/22 ,  C12N15/11

摘要： The present disclosure relates to systems and method of in-situ tissue profiling. Methods for spatiotemporal processing of a sample, capturing molecules of interest, and correlating cells in the sample to the capture molecules are provided.

公开(公告)号：US11180730B2

公开(公告)日：2021-11-23

申请号：US15966290

申请日：2018-04-30

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Brigham and Women's Hospital, Inc.

发明人： Aviv Regev ,  Ana Carrizosa Anderson ,  Le Cong ,  Vijay K. Kuchroo ,  Meromit Singer ,  Chao Wang

IPC分类号： C12N5/0783 ,  A61K35/17 ,  A61K39/00 ,  G01N33/50 ,  A61K45/06 ,  C12N15/10 ,  C12Q1/6883

摘要： The present invention provides markers, marker signatures and molecular targets that correlate with dysfunction of immune cells and are advantageously independent of the immune cell activation status. The present markers, marker signatures and molecular targets provide for new ways to evaluate and modulate immune responses. Specifically, GATA3 and/or FOXO1 modulation are provided for use as markers, marker signatures and molecular targets. Therapeutic methods are also provided to treat a patient in need thereof who would benefit from an increased immune response.

公开(公告)号：US11001622B2

公开(公告)日：2021-05-11

申请号：US15777054

申请日：2016-11-17

申请人： The Brigham and Women's Hospital, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Vijay K. Kuchroo ,  Chao Wang ,  Aviv Regev ,  Karthik Shekhar

IPC分类号： A61K38/00 ,  C07K14/705 ,  A61K38/20 ,  A61K38/17 ,  A61P37/00 ,  A61P1/00 ,  A61P35/00 ,  A61K39/395 ,  A61K45/06 ,  C07K14/54 ,  C07K16/24 ,  C07K16/28 ,  C12N15/113 ,  A61K39/00

摘要： Described herein are methods for suppressing an immune response in a subject, e.g., a subject with an autoimmune disease, by administering to the subject a therapeutically effective amount of recombinant CD5L, CD5L homodimers and/or CD5L:p40 heterodimers, or nucleic acids encoding any of these. Also described are methods for enhancing an immune response in a subject, e.g., a subject with cancer, infection, or an immune deficiency, by administering to the subject a therapeutically effective amount of an antibody or antigen-binding fragment thereof that binds specifically to CD5L, D5L homodimers and/or CD5L:p40 heterodimers, and inhibits their binding to the IL-23 receptor, or inhibits formation of the CD5L homodimer and/or CD5L:p40 heterodimer, or inhibitory nucleic acids that target CD5L and/or p40.

公开(公告)号：US20210121530A1

公开(公告)日：2021-04-29

申请号：US16497105

申请日：2018-03-23

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Brigham and Women's Hospital, Inc.

发明人： Antonia Wallrapp ,  Samantha J. Riesenfeld ,  Patrick R. Burkett ,  Monika S. Kowalczyk ,  Aviv Regev ,  Vijay K. Kuchroo

IPC分类号： A61K38/20 ,  A61K38/22 ,  A61K9/00 ,  C12Q1/6883 ,  A61P37/08

摘要： Computational and functional analysis identified the neuropeptide receptor Nmur1 as selectively expressed on Type 2 innate lymphoid cells (ILC2s). While both IL-33 and IL-25 promote ILC activation in vivo, IL-33 induces robust ILC proliferation, whereas ILCs activated with IL-25 do not proliferate as robustly and up-regulate Nmur1 expression. Treatment with neuromedin U (NMU), the neuropeptide ligand of Nmur1, had little effect on its own. Co-administration of IL-25 with NMU, however, dramatically amplified allergic lung inflammation and induced the proliferation and expansion of specific ILC2 subsets, characterized by a molecular signature unique to pro-inflammatory ILC2s. The results demonstrate that Nmur1 signaling strongly modulates IL-25-mediated ILC2 responses, resulting in highly proliferative pro-inflammatory ILCs, and highlights the importance of neuro-immune crosstalk in allergic inflammatory responses at mucosal surfaces.

公开(公告)号：US20200283843A1

公开(公告)日：2020-09-10

申请号：US16809458

申请日：2020-03-04

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Pratiksha Thakore ,  John Doench ,  JT Neal ,  Jesse Boehm ,  Oana Ursu

IPC分类号： C12Q1/6869 ,  C12N15/10

摘要： The present invention provides methods and tools for analyzing genetic interactions. The subject matter is generally directed to single-cell genomics and proteomics, including methods of performing genome-wide CRISPR perturbation screens and determining gene expression phenotypes.

公开(公告)号：US10655173B2

公开(公告)日：2020-05-19

申请号：US15030154

申请日：2014-10-17

申请人： THE BROAD INSTITUTE, INC. ,  Massachusetts Institute of Technology

发明人： Feng Zhang ,  Joshua Asher Weinstein ,  Aviv Regev

IPC分类号： C12Q1/6874 ,  C12Q1/6844

摘要： The present invention relates to molecular microscopy or volumetric imaging by proximal unique molecular identifiers (“UID”) reaction (“VIPUR”) microscopy methods to record the cellular co-localization and/or spatial distributions of arbitrary nucleic acid sequences, or other biomolecules tagged with nucleic sequences. The method involves one or both of two DNA sequence-components such as an α-UID, which may identify the targeted sequences-of-interest themselves and/or spatial beacons relative to which their distances are measured, and a- β-UID, which labels α-UID association events.

公开(公告)号：US20200152289A1

公开(公告)日：2020-05-14

申请号：US16681639

申请日：2019-11-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Brian Cleary ,  Aviv Regev ,  Eric S. Lander

IPC分类号： G16B25/10 ,  C12Q1/6874 ,  C12Q1/6806 ,  G16B50/50 ,  G06T5/50

摘要： The present invention relates to tissue and cell imaging utilizing genomic informatics and gene-expression profiling. Gene-expression profiles utilized in methods to obtain in situ imaging of cells and tissues provide complex molecular fingerprints regarding the relative state of a cell or tissue.