公开(公告)号：US20240269160A1

公开(公告)日：2024-08-15

申请号：US18418193

申请日：2024-01-19

Applicant: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

Inventor： Chen CHEN ,  Huaping LI ,  Daowen WANG ,  Beibei DAI

IPC: A61K31/7068 ,  A01K67/027 ,  A61P43/00 ,  C12N15/113 ,  C12N15/86

CPC classification number: A61K31/7068 ,  A01K67/027 ,  A61P43/00 ,  C12N15/113 ,  C12N15/86 ,  A01K2207/05 ,  A01K2267/0306 ,  C12N2310/122 ,  C12N2750/14143

Abstract: The present invention of use of citicoline in regulating respiratory chain function related protein level and preparing drug for preventing and treating respiratory chain dysfunction belongs to the field of drug. The present invention provides use of citicoline in regulating respiratory chain function related protein level, and also provides use of citicoline in preparing drug for preventing and treating respiratory chain dysfunction. The present invention also provides a shRNA causing respiratory chain dysfunction, its reverse complementary sequence is shown as SEQ ID NO. 2. The present invention also provides use of shRNA in preparing a respiratory chain dysfunction animal model, as well as its preparation method. It's confirmed by experiments that citicoline can effectively reverse molecular level symptoms of respiratory chain dysfunction and can be used as a drug for treating respiratory chain dysfunction.

公开(公告)号：US12031139B2

公开(公告)日：2024-07-09

申请号：US16538602

申请日：2019-08-12

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.

Inventor： Guy Reeves ,  Floyd Reed

IPC: C12N15/113 ,  A01K67/033 ,  C12N15/82

CPC classification number: C12N15/8218 ,  A01K67/033 ,  A01K67/0333 ,  A01K67/0339 ,  C12N15/113 ,  A01K2207/05 ,  A01K2217/15 ,  A01K2227/706 ,  A01K2267/02 ,  C12N2310/14

Abstract: The present invention relates to a method for reducing the competitive fitness of an organism hemizygous for a transgenic locus compared to the organism homozygous for the transgenic locus comprising the steps of: (a) reducing the expression of a haploinsufficient gene in the organism, wherein said reduction is conveyed by a transgenic locus in the organism; and (b) rescuing the reduced expression in the organism, wherein said rescue is conveyed by the same transgenic locus in the organism, yielding an organism which is less competitively fit if hemizygous for the transgenic locus than if homozygous for the transgenic locus. The present invention also relates to a method for decreasing the introgression of a transgenic locus in an organism into a population of otherwise interfertile sexually reproducing organisms comprising the steps of: (a) reducing the expression of a haploinsufficient gene in the organism, wherein said reduction is conveyed by a transgenic locus in the organism; (b) rescuing the reduced expression in the organism, wherein said rescue is conveyed by the same transgenic locus in the organism, and (c) using a transgenic organism obtained in step (b) in an environment comprising otherwise interfertile sexually reproducing wildtype individuals of the organism, wherein the competitive fitness of hemizygous progeny is reduced, thereby decreasing the rate of sexually reproduction and/or viability and/or the competitive fitness of hemizygous progeny. Further envisaged are corresponding genetic systems and genetically modified organisms.

公开(公告)号：US11938191B2

公开(公告)日：2024-03-26

申请号：US16864613

申请日：2020-05-01

Applicant: Genevant Sciences GmbH

Inventor： Sean D. Monahan ,  Michael S. Declue ,  Pierrot Harvie ,  Russell N. Johnson ,  Amber E. Paschal ,  Mary G. Prieve ,  Debashish Roy ,  Charbel Diab ,  Michael E. Houston, Jr. ,  Anna Galperin ,  Maher Qabar

IPC: A61K47/64 ,  A01K67/027 ,  A61K38/08 ,  A61K38/44 ,  A61K47/54 ,  A61K47/58 ,  A61K48/00 ,  C08F293/00 ,  C12N15/113 ,  C12N15/87

CPC classification number: A61K47/6455 ,  A01K67/027 ,  A61K38/08 ,  A61K38/44 ,  A61K47/549 ,  A61K47/58 ,  A61K48/0041 ,  C08F293/005 ,  C12N15/113 ,  C12N15/87 ,  A01K2207/05 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32 ,  C12Y113/12007

Abstract: Described herein are block copolymers, and methods of making and utilizing such copolymers. The described block copolymers are disruptive of a cellular membrane, including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the block copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the block copolymer is endosomolytic and capable of delivering an oligonucleotide (e.g., an mRNA) to a cell. Compositions comprising a block copolymer and an oligonucleotide (e.g., an mRNA) are also disclosed.

公开(公告)号：US20230257768A1

公开(公告)日：2023-08-17

申请号：US18009187

申请日：2021-06-11

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Isao OISHI ,  Kyoko YOSHII

IPC: C12N15/85 ,  A01K67/027

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K2207/05 ,  C12N2310/20

Abstract: According to the present invention, a poultry cell knocked-in at an egg white protein gene, a knock-in method, a method for producing a knocked-in poultry cell and an egg or a poultry containing the knocked-in poultry cell may be provided.

公开(公告)号：US10076579B2

公开(公告)日：2018-09-18

申请号：US15821594

申请日：2017-11-22

Applicant: Bell Biosystems, Inc.

Inventor： Caleb B. Bell, III ,  Alexey Bazarov

IPC: C12Q1/02 ,  A61K49/00 ,  G01N33/50 ,  A61K51/12 ,  A61K49/08 ,  A61K49/18 ,  C12N5/26 ,  C12Q1/6897 ,  C12N15/03 ,  B82Y5/00 ,  B82Y15/00

CPC classification number: A61K49/0097 ,  A01K2207/05 ,  A01K2207/12 ,  A61K49/08 ,  A61K49/1896 ,  A61K51/1203 ,  B82Y5/00 ,  B82Y15/00 ,  C12N5/166 ,  C12N15/03 ,  C12N2510/00 ,  C12Q1/689 ,  C12Q1/6897 ,  G01N33/5091

Abstract: The present invention is directed generally to eukaryotic cells comprising single-celled organisms that are introduced into the eukaryotic cell through human intervention and which transfer to daughter cells of the eukaryotic cell through at least five cell divisions, and methods of introducing such single-celled organisms into eukaryotic cells. The invention also provides methods of using such eukaryotic cells. The invention further provides single-celled organisms that introduce a phenotype to eukaryotic cells that is maintained in daughter cells. The invention additionally provides eukaryotic cells containing magnetotactic bacteria.

公开(公告)号：US09771430B2

公开(公告)日：2017-09-26

申请号：US14197173

申请日：2014-03-04

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： Ira Tabas ,  Lale Ozcan

IPC: A61K31/00 ,  C12N9/99 ,  C07K16/40 ,  A01K67/027 ,  C07K14/705 ,  C12N9/12 ,  A61K31/47 ,  A61K31/472 ,  C12N15/113 ,  G01N33/50

CPC classification number: C07K16/40 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2207/25 ,  A01K2217/075 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/0362 ,  A61K31/47 ,  A61K31/472 ,  C07K14/705 ,  C12N9/1205 ,  C12N15/1137 ,  G01N33/5023 ,  G01N2333/91215 ,  G01N2500/10 ,  G01N2800/04

Abstract: The present invention relates to methods of treating or preventing a metabolic disorder in a subject, methods of treating or preventing coronary artery disease in a subject with a metabolic disorder, as well as methods of reducing hepatic glucose production in a subject. Such methods include, but are not limited to, the administration to the subject of inhibitors or activators of CaMKII, IP3Rs, calcineurin, p38, MK2/3, HDAC4, Dach1, Dach2, or any combination thereof. The invention also provides methods of identifying a compound that inhibits the activity of CaMKII, IP3Rs, calcineurin, p38, MK2/3, HDAC4, Dach1 or Dach2, or reduces the activity and/or activation of CaMKII, IP3Rs, calcineurin, p38, MK2/3, HDAC4, Dach1 or Dach, or activates CaMKII, IP3Rs, calcineurin, p38, MK2/3, HDAC4, Dach1 or Dach2.

公开(公告)号：US20170166927A1

公开(公告)日：2017-06-15

申请号：US15423720

申请日：2017-02-03

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Phillip D. Zamore

IPC: C12N15/86 ,  A61K48/00 ,  A61K9/00

CPC classification number: A61K48/0008 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0331 ,  A61K9/0019 ,  A61K38/00 ,  A61K48/0058 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/8509 ,  C12N15/86 ,  C12N2015/8527 ,  C12N2310/13 ,  C12N2310/141 ,  C12N2320/32 ,  C12N2330/51 ,  C12N2710/10342 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2820/002 ,  C12N2840/007 ,  C12N2840/102

Abstract: The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA.

公开(公告)号：US09624494B2

公开(公告)日：2017-04-18

申请号：US14630419

申请日：2015-02-24

Applicant: Cold Spring Harbor Laboratory

Inventor： Gregory J. Hannon ,  Sihem Cheloufi

IPC: C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12N15/113 ,  C12N15/11

CPC classification number: C12N15/113 ,  A01K2207/05 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531 ,  C12N2320/30

Abstract: Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules.

公开(公告)号：US20160066549A9

公开(公告)日：2016-03-10

申请号：US14506690

申请日：2014-10-06

Applicant: Siu Ming CHAN

Inventor： Siu Ming CHAN

IPC: A01K61/00

CPC classification number: A01K61/005 ,  A01K61/59 ,  A01K61/90 ,  A01K2207/05 ,  A01K2207/10 ,  A01K2227/70 ,  A01K2267/02 ,  A61D19/027 ,  C12N15/1136 ,  C12N15/873 ,  Y02A40/824

Abstract: This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

公开(公告)号：US20160050894A1

公开(公告)日：2016-02-25

申请号：US14928463

申请日：2015-10-30

Applicant: Xavier LAUTH ,  John T. Buchanan T. BUCHANAN

Inventor： Xavier LAUTH ,  John T. Buchanan T. BUCHANAN

IPC: A01K67/027 ,  C12N15/85

CPC classification number: A01K67/0275 ,  A01K2207/05 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/03 ,  C07K14/4747 ,  C12N15/8509 ,  C12N2800/80 ,  Y02A90/40

Abstract: The present invention provides Maternal Sterility Constructs (MSC) and methods of producing sterile progeny lacking germ cells. Female fish carrying the MSC transgene will give rise to a sterile generation, as the MSC specifically eliminates Progenitor Germ Cells (PGCs) of her progeny. These females are called lineage ending females. Male fish carrying the MSC transgene, however, give rise to fertile progeny (assuming the male is not derived from an MSC-transgenic female). Thus, MSC transgenic males can be used to propagate the transgenic line. The invention can be advantageously applied to provide effective population control and improve culture performance of farmed species, such as fish.

Abstract translation: 本发明提供产妇无菌构建体（MSC）以及生产缺乏生殖细胞的无菌后代的方法。 携带MSC转基因的雌性鱼将产生无菌的产生，因为MSC特异性地消除了其后代的祖细胞（PGCs）。 这些女性被称为流血女性。 然而，携带MSC转基因的雄性鱼产生可育后代（假设雄性不是源于MSC转基因雌性）。 因此，MSC转基因雄性可以用于繁殖转基因品系。 本发明可有利地应用于提供有效的群体控制并改善养殖物种如鱼类的培养性能。