公开(公告)号：US11094040B2

公开(公告)日：2021-08-17

申请号：US16688284

申请日：2019-11-19

Applicant: Zhejiang University of Technology

Inventor： Wei Wu ,  Jiancheng Luo ,  Ying Shen ,  Tingting Chen ,  Weiwei Ge ,  Zhenqian Chen ,  Liegang Xia

IPC: G06T5/00 ,  G06K9/00 ,  G06K9/40 ,  H04N1/409

Abstract: A noise detection method for time-series vegetation index (TSVI) derived from remote sensing images. Firstly, unit root test is used to classify observation values of each pixel into a stationary series or a non-stationary series; for the non-stationary, an appropriate mathematical model is used to model discrete TSVI, then differences between actual observation values and prediction values of the model are calculated and recorded as a deviation. As the deviation has removed seasonal components, the non-stationary series is transformed into a stationary series. For a stationary series or deviation data, noise detection is performed based on the assumption that observation values are distributed within a certain range around mean values; then model fitting and noise detection are iteratively carried out with remained observation values—until the iterations reached the maximum number or no noise is detected at one iteration. The time series is then converted back to image space to obtain a noise mask and optimized. The present invention can obtain an accurate noise mask and improve reliability of land surface-related applications.

公开(公告)号：US11001823B2

公开(公告)日：2021-05-11

申请号：US16619344

申请日：2019-01-24

Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY

Inventor： Yaping Xue ,  Yuguo Zheng ,  Zhe Xu ,  Zhiqiang Liu

IPC: C12N9/78 ,  C12P13/00 ,  C12N15/00 ,  C12N1/20 ,  C12N15/63

Abstract: The present invention discloses a nitrilase mutant and application thereof. The mutant is obtained by mutating the amino acid at position 201 or replacing one or more amino acids at region 324-381 of the amino acid sequence shown in SEQ ID No. 2. In the present invention, by the protein molecular modification, thermostability of the purified nitrilase LNIT5 is increased by up to 4.5 folds; and by utilizing recombinant E. coli containing the nitrilase mutant to hydrolyze 1-cyanocyclohexylacetonitrile at a high temperature (45° C.), product tolerance is increased, activity of NIT5-L201F is increased by 20%, and the mutant NITLNIT5-AcN can completely hydrolyze 750 mM 1-cyanocyclohexylacetonitrile within 8 hours and achieve an doubled conversion rate. Therefore, the mutants obtained by the present invention have a good application prospect in efficiently catalyzing 1-cyanocyclohexylacetonitrile to synthesize gabapentin intermediate, 1-cyanocyclohexyl acetic acid. In the present invention, by protein molecular modification, thermal stability of pure nitrilase LNIT5 at 45° C. is increased up to 4.5 times, and while 1-cyanocyclohexylacetonitrile is hydrolyzed using recombinant Escherichia coli containing nitrilase mutant at high temperature (45° C.), the product yield is increased. Therefore, the mutants obtained in the present invention have a good application prospect in highly efficiently catalyzing 1-cyanocyclohexylacetonitrile to 1-cyanocyclohexyl acetic acid, the gabapentin intermediate.

公开(公告)号：US20210071215A1

公开(公告)日：2021-03-11

申请号：US16978345

申请日：2019-06-28

Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY

Inventor： Yaping XUE ,  Feng CHENG ,  Heng LI ,  Yuguo ZHENG ,  Jianmiao XU

IPC: C12P13/04 ,  C12N9/06

Abstract: The present invention discloses an amino acid dehydrogenase mutant and application thereof in synthesizing L-glufosinate-ammonium, the amino acid dehydrogenase mutant is obtained by a single mutation or a multi-site mutation of the amino acid at position 95, 108, 172, 303 of the amino acid sequence shown in SEQ ID No.2. The amino acid dehydrogenase mutant DyGDH-F95I-A108T-R172P-R303H prepared by the present invention has a specific enzyme activity that is 33 times higher than that of the original Aldo-keto reductase, and the concentration of the largest substrate, 2-carbonyl-4-(hydroxymethylphosphinyl)-butyric acid reaches 500 mM, the amino acid dehydrogenase mutant has more industrial application prospects. Using the amino acid dehydrogenase mutant to produce L-glufosinate-ammonium, the reaction time is significantly shortened, the general process takes 20 hours, and the reaction time of the present invention only requires 120 minutes, which shows that the amino acid dehydrogenase mutant has a good industrial application prospect.

公开(公告)号：US20210047609A1

公开(公告)日：2021-02-18

申请号：US16964999

申请日：2019-01-24

Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY ,  Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory

Inventor： Yuguo ZHENG ,  Xia KE ,  Yanghui LU ,  Zhongce HU ,  Yang WU

IPC: C12N1/20 ,  C12P17/02

Abstract: The present invention discloses a method for promoting the fermentation of Gluconobacter oxydans to produce D-sorbitol dehydrogenase and pyrroloquinoline quinone. The method comprises: Gluconobacter oxydans is inoculated to a fermentation culture medium, fermented and cultured under the conditions of 28-32° C. and 150-180 rpm for 6-24 hours, the fermented solution is centrifuged, and wet bacteria are collected, thus acquiring bacteria cells containing D-sorbitol dehydrogenase and pyrroloquinoline quinone. The method promotes the synthesis of coenzyme pQQ and the enzyme activity of per unit volume D-sorbitol dehydrogenase, Gluconobacter oxydans cultured and acquired using the method is biotransformed to synthesize miglitol precursor 6-deoxy-6-amino(N-hydroxyethyl)-α-L-furan sorbose (6NSL), the conversion progress of the product 6NSL is increased by 21-35%, and a biotransformation step cycle is reduced from 48 hours to 36 hours. In addition, under a same substrate concentration (60 g/L), the cumulative concentration of the product 6NSL is increased by 10 g/L or more.

公开(公告)号：US20210009981A1

公开(公告)日：2021-01-14

申请号：US16968808

申请日：2019-01-31

Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY

Inventor： RENCHAO ZHENG ,  YUGUO ZHENG ,  QIN ZHANG ,  ZHEMING WU ,  XIAOLING TANG ,  XIAFENG LU

IPC: C12N9/78 ,  C12N15/70 ,  C12P13/00

Abstract: The present invention discloses a nitrilase mutant and its construction method and its application in the synthesis of chiral intermediate of pregabalin in the technical field of bioengineering. The present invention, respectively, takes turnip nitrilase BrNIT and arabidopsis nitrilase AtNIT as parent, using peptide fragment displacement method, displaces the sites 226-286 of BrNIT amino acid sequence and sites 225-285 of AtNIT amino acid sequence with sites 225-285 of Arabis alpina L. nitrilase AaNIT, obtain nitrilase mutants BrNIT225-285 and AtNIT225-285 of which the amino acid sequence is as shown in SEQ ID NO.1 or SEQ ID NO.3. Compared with wild type nitrilase, the activity of the nitrilase mutant provided by the present invention in catalyzing and hydrolyzing racemic IBSN and the stereoselectivity of the product show substantial improvement, it can satisfy the requirements of industrial application, and has good application prospect in efficient catalysis of racemic IBSN to synthesize 3-cyano-5-methylhexanoic Acid.

公开(公告)号：US20190387773A1

公开(公告)日：2019-12-26

申请号：US16452516

申请日：2019-06-25

Applicant: Hangzhou Qiandao Lake Development Group Co., Ltd. ,  Zhejiang University of Technology

Inventor： Yuting DING ,  Shulai LIU ,  Zhenyu ZHANG ,  Jianmin WANG ,  Guangxi HE ,  Hongzheng YAO

IPC: A23L3/3472 ,  A23L3/358 ,  A23L3/349 ,  A23L3/3526

Abstract: The present invention discloses an antifreeze solution for food preservation, which comprises edible alcohol, propylene glycol, glycerin, calcium chloride, sodium chloride, amino acid, Antarctic krill protein hydrolysate with an average molecular weight of 50-100 KDa, surfactant and water, wherein the surfactant is one or more of phospholipid, Tween-20, Tween-60, and Tween-80; each component is food grade, and the mass percentage is expressed as follows: alcohol 15-30%, propylene glycol 10-30%, glycerol 2-15%, calcium chloride 1-10%, sodium chloride 3-10%, amino acid 0.1-0.15%, Antarctic krill protein hydrolysate 0.01-0.3%, surfactant 0.005-0.5%, and the balance is water. The antifreeze solution provided by the present invention can lower the freezing point, improve the heat transfer efficiency, inhibit the crystallization of antifreeze solution during the low temperature stirring process and improve the stability of the antifreeze solution.

公开(公告)号：US20190387760A1

公开(公告)日：2019-12-26

申请号：US16452508

申请日：2019-06-25

Applicant: Zhejiang University of Technology

Inventor： Yuting DING ,  Shulai LIU ,  Xuxia ZHOU ,  Jianhua LIU ,  Fei LV ,  Jianyou ZHANG ,  Yanbo WANG ,  Zhenyu ZHANG

IPC: A23B4/09 ,  A23B4/20

Abstract: A method for preserving aquatic products at sea includes the following steps: an on-board refrigeration system is used to refrigerate the antifreeze solution, and the antifreeze solution is used as a main cold source, and temperatures at centers of fish bodies drop rapidly to achieve rapid cooling and reduce activity of endogenous enzymes and inhibit proliferation of microorganisms by direct or indirect heat exchange with captured catches. The antifreeze solution contains edible alcohol, propylene glycol, glycerol, calcium chloride, sodium chloride, amino acids, Antarctic krill protein hydrolysate with average molecular weight of 50-100 KDa, surfactant and water, and the mass percentage of each component is as follows: edible alcohol 15%-30%, propylene glycol 10%-30%, glycerol 2%-15%, calcium chloride 1%-10%, sodium chloride 3%-10%, amino acid 0.1%-0.15%, Antarctic krill protein hydrolysate 0.01%-0.3%, surfactant 0.005%-0.5%, and the balance is water.

公开(公告)号：US10260059B2

公开(公告)日：2019-04-16

申请号：US15687800

申请日：2017-08-28

Applicant: Zhejiang University of Technology ,  Zhejiang Chiral Medicine Chemicals Co. Ltd.

Inventor： Renchao Zheng ,  Yuguo Zheng ,  Qin Zhang ,  Youming Huang ,  Jianfeng Weng ,  Tianchun Liu ,  Weirong Fan

IPC: C12N9/78 ,  C12P13/00

Abstract: The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.

公开(公告)号：US10246475B2

公开(公告)日：2019-04-02

申请号：US15709303

申请日：2017-09-19

Applicant: Zhejiang University of Technology ,  AAC Microtech (Changzhou) Co., Ltd.

Inventor： Guijie Li ,  Yuanbin She ,  Xiangdong Zhao ,  Shaohai Chen

IPC: C09K11/06 ,  C07F15/00 ,  H01L51/00 ,  C09K11/02 ,  H01L51/50

Abstract: Disclosed herein are multidentate dinuclear cyclometallated platinum complexes. The complexes are suitable as emitting materials or host materials in OLED devices, the materials having one of the following structures:

公开(公告)号：US10081590B2

公开(公告)日：2018-09-25

申请号：US15687470

申请日：2017-08-26

Applicant: Zhejiang University of Technology

Inventor： Yong Nie ,  Ying Duan ,  Ruchao Gong ,  Shangzhi Yu ,  Meizhen Lu ,  Jianbing Ji

IPC: C07C67/333 ,  B01J19/12 ,  B01J8/02

CPC classification number: C07C67/333 ,  B01J8/0285 ,  B01J19/126 ,  B01J2208/00176 ,  B01J2208/00442 ,  B01J2208/0053 ,  B01J2219/0801 ,  B01J2219/0869 ,  B01J2219/0871 ,  B01J2219/0892 ,  B01J2219/1215 ,  B01J2219/1245 ,  Y02P20/123 ,  C07C69/533

Abstract: A device and a process for producing undecylenic acid methyl ester using methyl ricinoleate as raw material are provided. The device comprises a feed pump, a raw material pre-heater, a microwave catalytic reactor, a microwave generator, a temperature controller and an infrared sensor, a condenser, a product tank and a discharge pump. The feed pump is connected with the raw material pre-heater, which is connected with the inlet of the microwave catalytic reactor. The outlet of the microwave catalytic reactor is connected with the condenser, which is connected to the product tank and the discharge pump. The microwave catalytic reactor is located in the microwave generator, which is connected with the temperature controller and the infrared sensor. The process is as follows: high-purity methyl ricinoleate, used as the raw material, is converted to methyl undecene and heptaldehyde by microwave-assisted pyrolysis process, followed by isolation and purification to produce methyl undecene.