公开(公告)号：US20070014769A1

公开(公告)日：2007-01-18

申请号：US11526429

申请日：2006-09-25

申请人： Frank Graham ,  Silvia Bacchetti ,  Philip Ng ,  Robin Parks ,  Mauro Anglana

发明人： Frank Graham ,  Silvia Bacchetti ,  Philip Ng ,  Robin Parks ,  Mauro Anglana

IPC分类号： A61K48/00 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/5256 ,  C12N7/00 ,  C12N9/22 ,  C12N15/85 ,  C12N2710/10343 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2840/203

摘要： The present invention relates to methods for efficient and reliable construction of adenovirus vectors which contain and express foreign DNA and are useful for gene transfer into mammalian cells, for vaccines and for gene therapy. The invention provides for the growth and purification of adenovirus vectors (helper dependent vectors or HDVs) from which all or most of the viral genes have been removed. The vector system described herein is a new method designed to eliminate helper viruses from the final HDV preparation by cleavage of the helper virus DNA with an endonuclease, alone or in combination with other methods known to limit the level of helper virus contamination of helper dependent vector preparations. The disclosed methods and compositions also provide for regulated control of gene expression.

摘要翻译： 本发明涉及用于有效和可靠地构建含有和表达外源DNA的腺病毒载体的方法，并且可用于将基因转移到哺乳动物细胞中，用于疫苗和基因治疗。 本发明提供了从其中除去所有或大部分病毒基因的腺病毒载体（辅助因子依赖载体或HDV）的生长和纯化。 本文描述的载体系统是设计用于通过用内切核酸酶单独或与已知限制辅助病毒污染辅助性依赖载体水平的其它方法结合的内切核酸酶切割辅助病毒DNA从最终HDV制备中消除辅助病毒的新方法 准备工作 所公开的方法和组合物还提供基因表达的调控。

公开(公告)号：US07135187B2

公开(公告)日：2006-11-14

申请号：US10355330

申请日：2003-01-31

申请人： Frank L. Graham ,  Philip Ng ,  Robin Parks

发明人： Frank L. Graham ,  Philip Ng ,  Robin Parks

IPC分类号： A61K39/23 ,  C07H21/04 ,  C12N7/00 ,  C12N15/00

CPC分类号： C12N15/86 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/85 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2840/203

摘要： The present invention relates to methods for efficient and reliable construction of adenovirus vectors which contain and express foreign DNA and are useful for gene transfer into mammalian cells, for vaccines and for gene therapy. The invention provides for the growth and purification of adenovirus vectors (helper dependent vectors or HDVs) from which all or most of the viral genes have been removed. The vector system described herein is a new method designed to eliminate helper viruses from the final HDV preparation by cleavage of the helper virus DNA with an endonuclease, alone or in combination with other methods known to limit the level of helper virus contamination of helper dependent vector preparations. The disclosed methods and compositions also provide for regulated control of gene expression.

公开(公告)号：US20060183232A1

公开(公告)日：2006-08-17

申请号：US11384850

申请日：2006-03-20

申请人： Ronald Vogels ,  Menzo Havenga ,  David Adrianus Zuijdgeest

发明人： Ronald Vogels ,  Menzo Havenga ,  David Adrianus Zuijdgeest

IPC分类号： C12N15/861 ,  C12N7/02

CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/38

摘要： In the absence of substantial sequence overlap between a recombinant adenoviral vector and the genome of a packaging cell, helper-dependent E1-containing particles (HDEP) can be formed at low frequency. The invention provides means and methods reducing or preventing the generation of HDEP. To this purpose, novel packaging cells and methods of making these are provided.

摘要翻译： 在重组腺病毒载体和包装细胞基因组之间不存在实质的序列重叠的情况下，可以以低频形成辅助依赖性的含有E1的颗粒（HDEP）。 本发明提供减少或防止HDEP产生的方法和方法。 为此，提供了新颖的包装电池及其制造方法。

公开(公告)号：US20060115807A1

公开(公告)日：2006-06-01

申请号：US10827684

申请日：2004-04-19

申请人： Ian Chambers

发明人： Ian Chambers

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N5/06 ,  C12N15/86

CPC分类号： C12N15/86 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2830/90 ,  C12N2840/203

摘要： A cell which expresses a replication factor is transfected with a vector which requires the presence of the replication factor to be maintained episomally within the cell. This is then expanded into a plurality of cells; and a cell in the plurality of cells selected (i) which maintains the vector episomally, and (ii) in which the vector has not integrated into chromosomal DNA of the cell.

摘要翻译： 用载体转染表达复制因子的细胞，所述载体需要复制因子的存在以保持在细胞内。 然后将其扩展成多个单元; 和多个细胞中的细胞，其选自（i），其维持所述载体的全身性，和（ii）其中所述载体未整合到细胞的染色体DNA中。

公开(公告)号：US07045347B2

公开(公告)日：2006-05-16

申请号：US10206163

申请日：2002-07-26

申请人： Frank L. Graham ,  Michael A. Rudnicki ,  Martina Anton

发明人： Frank L. Graham ,  Michael A. Rudnicki ,  Martina Anton

IPC分类号： C12N15/861 ,  C12N7/00 ,  C12N7/01 ,  A61K31/713 ,  A61K48/00

CPC分类号： C12N7/00 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/0036 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2800/30 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2840/203

摘要： This invention provides helper-dependent adenovirus cloning vectors and helper adenoviruses, and methods for making and Using such preparations, wherein the helper adenoviruses contain recombinase target sites that are useful in reducing the level of contamination of helper virus in helper-dependent adenovirus vector preparations.

公开(公告)号：US20040266005A1

公开(公告)日：2004-12-30

申请号：US10821726

申请日：2004-04-08

申请人： BENITEC AUSTRALIA LIMITED

发明人： Michael Wayne Graham ,  Robert Norman Rice

IPC分类号： C12N015/85 ,  C07H021/04

CPC分类号： C12N15/85 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/113 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/102 ,  C12N2840/20

摘要： The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.

摘要翻译： 本发明一般涉及用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中的内源基因表达的合成基因。 更具体地说，本发明提供了新的合成基因和遗传构建体，其能够在引入生物体时抑制延迟或以其他方式降低生物体中的内源基因或靶基因的表达。

公开(公告)号：US20040237145A1

公开(公告)日：2004-11-25

申请号：US10821710

申请日：2004-04-08

发明人： Michael Wayne Graham ,  Robert Norman Rice

IPC分类号： A01H001/00 ,  C12N015/82

CPC分类号： C12N15/113 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/10 ,  C12N2320/30 ,  C12N2330/30 ,  C12N2330/50 ,  C12N2330/51 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/20

摘要： The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endo gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the invention utilises recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in tissue, organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are cap repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced are also provided.

摘要翻译： 本发明一般涉及一种改变基因表达的方法和用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中内源基因表达的合成基因。 更具体地，本发明利用重组DNA技术来转录后修饰或调节组织，器官或整个生物体中靶基因的表达，从而产生新的表型。 还提供了当引入时，限制性抑制延迟或以其他方式降低生物体中的内源基因或靶基因的表达的新型合成基因和遗传构建体。

公开(公告)号：US20040110295A1

公开(公告)日：2004-06-10

申请号：US10446629

申请日：2003-05-28

申请人： Maxygen, Inc., a Delaware corporation

发明人： Juha Punnonen ,  Doris Apt ,  Robert G. Whalen

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N015/85

CPC分类号： C07K14/005 ,  A61K2039/53 ,  C12N15/85 ,  C12N2770/24122 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2840/20 ,  C12N2840/203 ,  Y02A50/386

摘要： The invention relates to nucleic acid vectors useful for expression and production of polypeptides, compositions comprising vectors, and methods for the production and use of vectors and polypeptides.

摘要翻译： 本发明涉及可用于表达和产生多肽的核酸载体，包含载体的组合物，以及用于产生和使用载体和多肽的方法。

公开(公告)号：US20040087029A1

公开(公告)日：2004-05-06

申请号：US10381153

申请日：2003-10-09

发明人： Jeffrey S. Chamberlain ,  Dennis J. Hartigan-O'Connor

IPC分类号： C12N015/861 ,  C12N007/00

CPC分类号： C12N15/86 ,  C12N2710/10043 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2800/30 ,  C12N2830/002 ,  C12N2830/38

摘要： The present invention relates to methods and compositions for the production of viral vectors. In particular, the present invention provides methods and compositions for faster, higher titer and higher purity production of viral vectors (e.g. adenoviral vectors). In some embodiments, the present invention provides gutted and helper viruses with identical or similar termini. In other embodiments, the present invention provides terminal protein linked adenoviral DNA. In certain embodiments, the present invention provides template extended adenoviral DNA.

摘要翻译： 本发明涉及用于生产病毒载体的方法和组合物。 特别地，本发明提供了用于更快，更高滴定度和更高纯度产生病毒载体（例如腺病毒载体）的方法和组合物。 在一些实施方案中，本发明提供具有相同或相似末端的内切和辅助病毒。 在其它实施方案中，本发明提供了末端蛋白质连接的腺病毒DNA。 在某些实施方案中，本发明提供扩增模板的腺病毒DNA。

公开(公告)号：US20040028653A1

公开(公告)日：2004-02-12

申请号：US10384136

申请日：2003-03-07

发明人： Brian Seed ,  Mason Wright Freeman ,  Alexander Kovtun ,  Masahiro Murakawa ,  Eun-Chung Park ,  Xinzhong Wang

IPC分类号： A61K048/00 ,  C12N015/861 ,  C12N007/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N15/63 ,  C12N15/635 ,  C12N2710/10343 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/205 ,  C12N2830/38 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/44

摘要： Disclosed are replicatable viral DNA vectors encoding a site-specific DNA-altering enzyme and a DNA target recognized by the enzyme, the enzyme selectively converting, in a cell expressing the enzyme, the DNA vector to a rearranged form. The invention further relates to methods for assembling recombinant adenoviral DNAs. These methods include the steps of: (a) providing a first linearized DNA vector including a restriction site and a cos site and a second linearized DNA vector including the restriction site, an adenoviral nucleic acid molecule, and a cos site; and (b) ligating the first and second linearized DNA vectors, the ligation assembling a recombinant adenoviral DNA.

摘要翻译： 公开的是可复制的病毒DNA载体，其编码位点特异性DNA改变酶和被酶识别的DNA靶，该酶在表达该酶的细胞中选择性地转化为重排的形式。 本发明还涉及组装重组腺病毒DNA的方法。 这些方法包括以下步骤：（a）提供包括限制性位点和cos位点的第一线性化DNA载体和包含限制性位点，腺病毒核酸分子和cos位点的第二线性化DNA载体; 和（b）连接第一和第二线性化DNA载体，连接装配重组腺病毒DNA。