公开(公告)号：US20050004056A1

公开(公告)日：2005-01-06

申请号：US10497692

申请日：2002-12-04

申请人： Martin Meise ,  Karsten Eulenberg ,  Rudiger Fritsch ,  Thomas Hader ,  Gunter Bronner ,  Arnd Steuernagel

发明人： Martin Meise ,  Karsten Eulenberg ,  Rudiger Fritsch ,  Thomas Hader ,  Gunter Bronner ,  Arnd Steuernagel

IPC分类号： A01K67/027 ,  A61K31/7088 ,  A61K31/7115 ,  A61K38/00 ,  A61K38/45 ,  A61K38/46 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P1/16 ,  A61P3/04 ,  A61P3/06 ,  A61P3/10 ,  A61P9/10 ,  A61P9/12 ,  A61P19/02 ,  A61P35/00 ,  C12N5/10 ,  C12N9/12 ,  C12N9/16 ,  C12N15/09 ,  C12Q1/42 ,  C12Q1/48 ,  G01N33/15 ,  G01N33/50

CPC分类号： C12N9/1205 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/00 ,  C12N9/16 ,  C12Q1/42 ,  C12Q1/485 ,  C12Y301/03048 ,  G01N2500/02 ,  G01N2500/04

摘要： The present invention discloses PTP10D, Tec protein tyrosine kinase, or EDTP homologous proteins regulating the energy homeostasis and the metabolism of triglycerides, and polynucleotides, which identify and encode the proteins disclosed in this invention. The invention also relates to the use of these sequences in the diagnosis, study, prevention, and treatment of diseases and disorders, for example, but not limited to, metabolic diseases such as obesity as well as related disorders such as eating disorder, cachexia, diabetes mellitus, hypertension, coronary heart disease, hypercholesterolemia, dyslipidemia, osteoarthritis, gallstones, cancers, e.g. cancers of the reproductive organs, and sleep apnea.

摘要翻译： 本发明公开了PTP10D，Tec蛋白酪氨酸激酶或调节甘油三酯的能量稳态和代谢的EDTP同源蛋白，以及鉴定和编码本发明公开的蛋白质的多核苷酸。 本发明还涉及这些序列在疾病和病症的诊断，研究，预防和治疗中的用途，所述疾病和病症例如但不限于代谢性疾病如肥胖以及相关疾病如进食障碍，恶病质， 糖尿病，高血压，冠心病，高胆固醇血症，血脂异常，骨关节炎，胆结石，癌症，例如 生殖器官的癌症和​​睡眠呼吸暂停。

公开(公告)号：US20040115635A1

公开(公告)日：2004-06-17

申请号：US10317401

申请日：2002-12-11

申请人： Isis Pharmaceuticals Inc.

发明人： Lex M. Cowsert ,  Kenneth W. Dobie

IPC分类号： C12Q001/68 ,  C07H021/04 ,  A61K048/00

CPC分类号： C12N15/1137 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/16 ,  C12Y301/03048 ,  C12N2310/3525

摘要： Compounds, compositions and methods are provided for modulating the expression of PTPN13. The compositions comprise oligonucleotides, targeted to nucleic acid encoding PTPN13. Methods of using these compounds for modulation of PTPN13 expression and for diagnosis and treatment of disease associated with expression of PTPN13 are provided.

摘要翻译： 提供化合物，组合物和方法用于调节PTPN13的表达。 组合物包含针对编码PTPN13的核酸的寡核苷酸。 提供了使用这些化合物调节PTPN13表达和诊断和治疗与PTPN13表达相关的疾病的方法。

公开(公告)号：US20040077574A1

公开(公告)日：2004-04-22

申请号：US10444795

申请日：2003-05-23

申请人： CEPTYR, Inc.

发明人： Richard Klinghoffer ,  Stephen Patrick Lewis

IPC分类号： A61K048/00 ,  C07H021/02 ,  C12N015/85

CPC分类号： C12N15/1137 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/3517 ,  C12N2310/53 ,  C12Y301/03048

摘要： Compositions and methods relating to small interfering RNA (siRNA) polynucleotides are provided as pertains to modulation of biological signal transduction. Shown are siRNA polynucleotides that interfere with expression of members of the protein tyrosine phosphatase (PTP) class of enzymes that mediate signal transduction, and with certain MAP kinase kinases (MKK). In certain preferred embodiments siRNA modulate signal transduction pathways comprising SHP2, cdc14a/b, cdc25A/B/C, KAP, PTP-null, PRL-3, CD45, dual specificity phosphatase-3 (DSP-3), MKK-4, and/or MKK-7. Modulation of PTP-mediated biological signal transduction has uses in diseases associated with defects in cell proliferation, cell differentiation and/or cell survival, such as metabolic disorders (including diabetes and obesity), cancer, autoimmune disease, infectious and inflammatory disorders and other conditions. The invention also provides siRNA polynucleotides that interfere with expression of chemotherapeutic target polypeptides, such as DHFR, thymidylate synthetase, and topoisomerase I.

摘要翻译： 提供了与小干扰RNA（siRNA）多核苷酸相关的组合物和方法，涉及生物信号转导的调节。 显示干扰介导信号转导的酶的蛋白酪氨酸磷酸酶（PTP）类成员和某些MAP激酶激酶（MKK）的成员的表达的siRNA多核苷酸。 在某些优选实施方案中，siRNA调节包含SHP2，cdc14a / b，cdc25A / B / C，KAP，PTP-ε，PRL-3，CD45，双特异性磷酸酶-3（DSP-3），MKK-4和 /或MKK-7。 PTP介导的生物信号转导的调节在与细胞增殖，细胞分化和/或细胞存活缺陷相关的疾病中使用，例如代谢紊乱（包括糖尿病和肥胖症），癌症，自身免疫性疾病，感染性和炎症性疾病和其他病症 。 本发明还提供干扰化学治疗靶多肽如DHFR，胸苷酸合成酶和拓扑异构酶I的表达的siRNA多核苷酸。

公开(公告)号：US20040014956A1

公开(公告)日：2004-01-22

申请号：US10357529

申请日：2003-02-03

申请人： Sequitur, Inc.

发明人： Tod M. Woolf ,  Kristin A. Wiederholt

IPC分类号： C07H021/02 ,  C07H021/04

CPC分类号： C12N15/1137 ,  A61K31/713 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2320/31 ,  C12N2320/50 ,  C12N2330/30 ,  C12Y207/11022 ,  C12Y301/03048

摘要： Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.

摘要翻译： 公开了具有比现有技术中教导的那些改进的性能的反义序列，包括双链RNAi组合物。 本发明提供了在体外系统和治疗上制备和使用这两者的优化的反义寡聚体组合物和方法。 本发明还提供了制备和使用改进的反义寡聚体组合物的方法。

公开(公告)号：US20040010045A1

公开(公告)日：2004-01-15

申请号：US10427887

申请日：2003-05-01

发明人： Taolin Yi

IPC分类号： A61K031/155

CPC分类号： A61K31/555 ,  A61K31/155 ,  A61K31/29 ,  A61K31/496 ,  C12N9/16 ,  C12Q1/42 ,  C12Y301/03048 ,  G01N33/57484 ,  A61K2300/00

摘要： Pentamidine, an anti-protozoa drug, is described herein as a potent PTPase inhibitor with anti-cancer activity. Pentamidine at its therapeutic doses inhibits recombinant PRL phosphatases and inactivates intracellular PRLs in NIH3T3 transfectants. Pentamidine treatment at a nontoxic dose markedly inhibits the growth of WM9 human melanoma tumors in nude mice coincident with tumor cell necrosis and is capable of inactivating an ectopically expressed PRL-2 in the cancer cells. The drug has growth inhibitory activity against different human cancer cell lines that express the PRLs, and therefore has broad anti-cancer activity based on inactivating the oncogenic phosphatases.

摘要翻译： 戊脒是抗原生动物药物，在本文中被描述为具有抗癌活性的有效的PTPase抑制剂。 Pentamidine在其治疗剂量下抑制重组PRL磷酸酶，并使NIH3T3转染子中的细胞内PRL失活。 在无毒剂量下的戊脒处理显着抑制与肿瘤细胞坏死相符的裸小鼠中WM9人黑素瘤肿瘤的生长，并且能够灭活癌细胞中异位表达的PRL-2。 该药物对表达PRL的不同人类癌细胞系具有生长抑制活性，因此具有广泛的抗癌活性，其基于致死性致癌性磷酸酶的失活。

公开(公告)号：US20030220282A1

公开(公告)日：2003-11-27

申请号：US10360510

申请日：2003-02-07

申请人： ISIS Pharmaceuticals, Inc.

发明人： Sanjay Bhanot ,  Lex M. Cowsert ,  Jacqueline R. Wyatt ,  Brett P. Monia ,  Madeline M. Butler ,  Robert McKay ,  Susan M. Freier

IPC分类号： A61K048/00 ,  C07H021/04

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346 ,  C12Y301/03048 ,  Y02P20/582 ,  C12N2310/3525

摘要： Compounds, compositions and methods are provided for modulating the expression of PTP1B. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding PTP1B. Methods of using these compounds for modulation of PTP1B expression and for treatment of diseases associated with expression of PTP1B are provided.

公开(公告)号：US20030138931A1

公开(公告)日：2003-07-24

申请号：US10346356

申请日：2003-01-15

申请人： CEPTYR, Inc.

发明人： Ralf M. Luche ,  Bo Wei

IPC分类号： C12N009/12 ,  C12N009/16 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC分类号： C12Q1/42 ,  A61K38/00 ,  C12N9/16 ,  C12Y301/03016 ,  C12Y301/03048 ,  G01N33/573 ,  G01N2500/04

摘要： Compositions and methods are provided for the treatment of conditions associated with cell proliferation, cell differentiation and cell survival. In particular, the dual-specificity phosphatase DSP-10, and polypeptide variants thereof that stimulate dephosphorylation of DSP-10 substrates, are provided. The polypeptides may be used, for example, to identify antibodies and other agents that inhibit DSP-10 activity. The polypeptides and agents may be used to modulate cell proliferation, differentiation and survival.

公开(公告)号：US20030119045A1

公开(公告)日：2003-06-26

申请号：US10314058

申请日：2002-12-05

申请人： Ceptyr, Inc.

发明人： Ralf M. Luche ,  Bo Wei

IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06 ,  C07K016/40 ,  A61K039/395 ,  C12P019/34 ,  C12N009/16

CPC分类号： C12Y301/03016 ,  C12N9/16 ,  C12Y301/03048

摘要： Compositions and methods are provided for the treatment of conditions associated with cell proliferation, cell differentiation and cell survival. In particular, the dual-specificity phosphatase DSP-9, and polypeptide variants thereof that stimulate dephosphorylation of DSP-9 substrates, are provided. The polypeptides may be used, for example, to identify antibodies and other agents that inhibit DSP-9 activity. The polypeptides and agents may be used to modulate cell proliferation, differentiation and survival.

摘要翻译： 提供组合物和方法用于治疗与细胞增殖，细胞分化和细胞存活相关的病症。 特别地，提供了刺激DSP-9底物去磷酸化的双重特异性磷酸酶DSP-9及其多肽变体。 多肽可用于例如鉴定抑制DSP-9活性的抗体和其它试剂。 多肽和试剂可用于调节细胞增殖，分化和存活。

公开(公告)号：US20030105308A1

公开(公告)日：2003-06-05

申请号：US09918728

申请日：2001-07-31

发明人： Leonid Beigelman ,  Shawn Zinnen

IPC分类号： C12Q001/68 ,  C07H021/02

CPC分类号： C07H19/10 ,  A61K38/00 ,  C07H19/20 ,  C07H21/00 ,  C12N15/113 ,  C12N15/1131 ,  C12N15/1135 ,  C12N15/1137 ,  C12N15/1138 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/121 ,  C12N2310/122 ,  C12N2310/18 ,  C12N2310/315 ,  C12N2310/317 ,  C12N2310/318 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/33 ,  C12N2310/332 ,  C12N2310/345 ,  C12N2310/346 ,  C12P19/30 ,  C12P19/305 ,  C12Y204/02001 ,  C12Y207/01037 ,  C12Y207/07049 ,  C12Y301/03048 ,  C12N2310/3523 ,  C12N2310/3521

摘要： The present invention relates to novel nucleotide triphosphates, methods of synthesis and process of incorporating these nucleotide triphosphates into oligonucleotides, and isolation of novel nucleic acid catalysts (e.g., ribozymes or DNAzymes). Also, provided are the use of novel enzymatic nucleic acid molecules to inhibit gene expression and their applications in human therapy.

摘要翻译： 本发明涉及新的三磷酸核苷酸，合成方法以及将这些三磷酸核苷酸掺入寡核苷酸的方法，以及分离新的核酸催化剂（例如核酶或DNA酶）。 另外，提供了新的酶核酸分子用于抑制基因表达及其在人类治疗中的应用。

公开(公告)号：US20030049609A1

公开(公告)日：2003-03-13

申请号：US10191029

申请日：2002-07-03

发明人： Craig A. Smith ,  Raymond G. Goodwin

IPC分类号： C12Q001/70 ,  C07H021/04 ,  C12P021/06 ,  C12P019/34 ,  A61K039/12 ,  C07K002/00 ,  C07K004/00 ,  C07K005/00 ,  C07K007/00 ,  C07K014/00 ,  C07K016/00 ,  C07K017/00 ,  A61K038/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C12N9/16 ,  C12N2710/24122 ,  C12Y301/03048

摘要： A poxvirus protein designated A41L binds to leukocyte common-antigen-related protein (LAR). A41L is a secreted protein that can be purified from the culture supernatant of cells infected with certain poxviruses, or produced using recombinant DNA techniques. A41L polypeptides and LAR polypeptides, and nucleic acids encoding them, are provided herein. Also provided are methods of using such polypeptides and nucleic acids.

摘要翻译： 命名为A41L的痘病毒蛋白结合白细胞共同抗原相关蛋白（LAR）。 A41L是可以从用某些痘病毒感染的细胞的培养物上清液纯化的分泌蛋白质，或使用重组DNA技术产生的。 A41L多肽和LAR多肽，以及编码它们的核酸。 还提供了使用这种多肽和核酸的方法。