公开(公告)号：US20180001974A1

公开(公告)日：2018-01-04

申请号：US15706417

申请日：2017-09-15

申请人： S. P. Cramer & Associates d/b/a Cramer Fish Sciences

发明人： Joseph Merz

IPC分类号： B63B35/34 ,  B63B35/00 ,  G01N1/12 ,  G01N33/18 ,  G01N1/40 ,  G01N1/10

CPC分类号： B63B35/34 ,  B63B35/00 ,  G01N1/12 ,  G01N33/18 ,  G01N33/186 ,  G01N2001/1025 ,  G01N2001/1093 ,  G01N2001/4088

摘要： Low or no disturbance sampling can be accomplished such as through a single-platform aquatic species and habitat sampling system with data integration and rapid processing capabilities that can address the need for sampling at variable depths over varied habitats, along with the simultaneous collection of linked physical and biological data. The platform may be based on a 24-36 foot boat, and may include a net mouth opener brace for an adjustable concentrator net and smaller drift net which may be attached to an adjustable sample chamber, perhaps containing variable mesh capture nets as well as cameras, water sampling equipment, and water quality sensors integrated with a fish finder, GPS, and other monitoring and data recording equipment. The depth of the net mouth opener brace and sample chamber may be adjustable using a depth control.

公开(公告)号：US09513194B2

公开(公告)日：2016-12-06

申请号：US13698278

申请日：2011-05-18

申请人： Darcilio Fernandes Baptista ,  Riccardo Mugnai ,  Renata Bley De Oliveira

发明人： Darcilio Fernandes Baptista ,  Riccardo Mugnai ,  Renata Bley De Oliveira

IPC分类号： G01N1/22 ,  G01N1/10 ,  G01N1/12 ,  G01N1/18 ,  G01N33/18 ,  A01K61/00

CPC分类号： G01N1/10 ,  A01K61/90 ,  G01N1/12 ,  G01N1/18 ,  G01N33/186 ,  G01N2001/1012

摘要： The present invention refers to the subsampler and to a subsampling method that allows for the execution of environmental monitoring without the use of large sample volumes, thus ensuring specimen wealth and expedited analyses.

摘要翻译： 本发明涉及二次采样器和二次采样方法，其允许在不使用大样本量的情况下执行环境监测，从而确保标本财富和加速分析。

公开(公告)号：US09194854B2

公开(公告)日：2015-11-24

申请号：US13577180

申请日：2011-02-07

申请人： Odd Ketil Andersen

发明人： Odd Ketil Andersen

IPC分类号： G01N33/18 ,  G01N21/63

CPC分类号： G01N33/18 ,  G01N33/186 ,  G01N2021/635

摘要： The effects of pollution in an aquatic mass may be monitored by measuring changes in various parameters of sessile organisms, particularly bivalves such as mussels. Particular parameters measured include particle clearance rate, oxygen consumption and apical growth.

摘要翻译： 可以通过测量无柄生物体，特别是双壳类如贻贝的各种参数的变化来监测水生物质中污染的影响。 测量的特定参数包括颗粒清除率，氧消耗和顶端生长。

公开(公告)号：US09057717B2

公开(公告)日：2015-06-16

申请号：US13512285

申请日：2010-11-26

申请人： Odd Ketil Andersen

发明人： Odd Ketil Andersen

IPC分类号： A01K61/00 ,  G01N33/18 ,  G01N21/47

CPC分类号： G01N33/186 ,  G01N21/4788

摘要： A method of monitoring the effect of pollution in an aquatic mass, said method comprising disposing in said aquatic mass a biosensor unit containing a living sessile organism which exhibits apical growth and which is bound to a carrier, directing an electromagnetic beam towards a corresponding detector and between an edge and the apical tip of said sessile organism whereby to produce a diffraction pattern, detecting said diffraction pattern using said detector and monitoring a change (e.g. successive changes) in said diffraction pattern over time which is indicative of the natural growth of the apical tip of said organism.

摘要翻译： 一种监测水生质量污染影响的方法，所述方法包括在所述水生物质中放置生物传感器单元，所述生物传感器单元含有表现为顶端生长并与载体结合的生物固定生物体，将电磁波束导向相应的检测器， 在所述无柄生物体的边缘和顶端之间，从而产生衍射图案，使用所述检测器检测所述衍射图案并监测随着时间的所述衍射图案中的变化（例如连续变化），其指示顶端的自然生长 所述生物体的尖端。

公开(公告)号：US20150141271A1

公开(公告)日：2015-05-21

申请号：US14476709

申请日：2014-09-03

申请人： Nanjing University

发明人： Yan ZHANG ,  Hongqiang REN ,  Bing WU ,  Xuxiang ZHANG

IPC分类号： C12Q1/68 ,  G01N33/18 ,  G06F19/20 ,  G01N24/08

CPC分类号： C12Q1/6883 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N24/088 ,  G01N33/18 ,  G01N33/1813 ,  G01N33/186 ,  G01N33/50 ,  G01N2570/00 ,  G06F19/20

摘要： A method for analyzing water toxicity, the method including: exposure experiment, sample collection, transcriptome detection, metabolome detection, screening of differentially expressed genes, screening of differentially expressed metabolites, and identification of commonly changed biological pathways in both the transcriptome and the metabolome.

摘要翻译： 一种分析水毒性的方法，其包括：暴露实验，样品收集，转录组检测，代谢组学检测，差异表达基因筛选，筛选差异表达的代谢物，以及鉴定转录组和代谢组中常见的生物学途径。

公开(公告)号：US20120086802A1

公开(公告)日：2012-04-12

申请号：US12936716

申请日：2009-04-09

申请人： How Lung Eng ,  Beng Hai Lee ,  Suryanti Yunita Anggrelly ,  Boon Fong Chew

发明人： How Lung Eng ,  Beng Hai Lee ,  Suryanti Yunita Anggrelly ,  Boon Fong Chew

IPC分类号： H04N7/18

CPC分类号： C02F1/00 ,  A01K61/95 ,  C02F2209/00 ,  G01N33/186

摘要： A system and system for monitoring water quality. The system comprises a container for receiving a flow of water to be monitored, the container containing a plurality of fish and configured such that a substantially 3-dimensional group behaviour of the fish is accommodated; a first imaging device disposed above the container for obtaining top view video data of the fish; and means for identifying individual fish based on foreground object detection.

摘要翻译： 一个用于监测水质的系统和系统。 该系统包括用于接收待监测的水流的容器，容器包含多个鱼，并且构造成容纳鱼的基本上3维组的行为; 设置在所述容器上方的用于获得所述鱼的顶视图数据的第一成像装置; 以及用于基于前景物体检测识别个体鱼的装置。

公开(公告)号：US07330794B2

公开(公告)日：2008-02-12

申请号：US10343660

申请日：2001-07-30

申请人： Jason Ezratty ,  Robert Morgan ,  David Eddy ,  Donald Morgan

发明人： Jason Ezratty ,  Robert Morgan ,  David Eddy ,  Donald Morgan

IPC分类号： G06F19/00 ,  C12Q1/00 ,  C12N5/02 ,  G01F1/12

CPC分类号： G01N33/186 ,  G01N2520/00

摘要： A toxicant detection system comprises at least one watertight chamber containing a mollusk. Water to be screened is introduced into the chamber. The mollusk is preferably supported on a mounting structure which facilitates quick mounting of the mollusk in the device, comprising a releasable element affixed to the shell of the mollusk, e.g., a bolt which is screwed into a nut affixed to the mollusk's shell. A sensing apparatus is provided which includes a movable member supported in the chamber so that it engages the shell of the mollusk, and moves when the shell opens and closes. The sensing apparatus detects the position of the mollusk shell based on the position of the movable member. The sensing apparatus preferably includes a Hall effect transducer which co-acts with a magnet carried on the movable member. The system allows for accurate readings of the small movements of the mollusk shell which aid in detecting toxicants, and also provides for installation of mollusks of varying sizes, without the need to move components of the standard configuration of the chamber. Methods and systems for detecting or identifying a toxicant in water being screened are also described.

摘要翻译： 毒物检测系统包括至少一个包含软体动物的水密室。 要被筛选的水被引入室内。 软体动物优选地支撑在便于将软体动物快速安装在装置中的安装结构上，该安装结构包括固定在软体动物的壳体上的可释放元件，例如拧入固定在软体动物壳上的螺母的螺栓。 提供了一种感测装置，其包括支撑在室中的可动构件，使得其接合软体动物的壳体，并且当壳体打开和关闭时移动。 感测装置基于可动构件的位置来检测软体动物壳的位置。 感测装置优选地包括霍尔效应传感器，其与携带在可动构件上的磁体共同作用。 该系统允许准确读取软体动物壳的小的运动，其有助于检测毒物，并且还提供安装不同尺寸的软体动物，而不需要移动腔室的标准构造的部件。 还描述了用于检测或鉴定被筛选的水中的毒物的方法和系统。

公开(公告)号：US20060143718A1

公开(公告)日：2006-06-29

申请号：US11286613

申请日：2005-11-23

申请人： Daniel Nebert

发明人： Daniel Nebert

IPC分类号： A01K67/027

CPC分类号： G01N33/5308 ,  G01N21/763 ,  G01N33/186 ,  G01N33/5088 ,  G01N2520/00

摘要： The present invention provides methods and systems that uses transgenic zebrafish with an easily assessable reporter gene under the control of pollutant-inducible DNA response elements. Transgenic zebrafish, carrying pollution-inducible response elements, are placed in the water to be tested, and the contaminants become bioconcentrated (generally 1,000- to 40,000-fold, relative to the water) in the tissues of the fish thereby activating specific response elements, which up-regulate the LUC reporter gene. Fish are then removed from the test water and placed immediately in a luminometer cuvette and incubated with luciferin. Luciferin is rapidly taken up into the tissues of the fish, oxidized by luciferase, and light is produced. The luminescence is proportional to the environmental concentration of the pollutant (to which the fish had been exposed), which drives the expression of the LUC gene by means of the various DNA motifs. The luminescence is quantitated in the luminometer. In each response element-containing construct, a specific class of polluting chemicals, allowing for differential identification of pollutants in a complex mixture activates the expression of the LUC gene. This assay does not require killing the fish and allows for repeated analysis of the same site with the same fish. The sensitivity of the system can be manipulated by varying the sequence of the response element.

摘要翻译： 本发明提供了在污染诱导型DNA应答元件控制下使用转基因斑马鱼与容易评估的报道基因的方法和系统。 带有污染诱导型应答元件的转基因斑马鱼被放置在被测试的水中，并且污染物在鱼的组织中变得生物浓缩（相对于水）为生物浓缩物（通常为1,000至40,000倍），从而激活特定的应答元件， 其上调LUC报告基因。 然后将鱼从测试水中取出并立即放置在发光计比色皿中并与荧光素一起温育。 萤光素被迅速吸收到鱼的组织中，被荧光素酶氧化，产生光。 发光与污染物的环境浓度（鱼已暴露于其中）成比例，通过各种DNA图案驱动LUC基因的表达。 在发光计中定量发光。 在每个含有响应元素的构造中，特定类别的污染化学品允许复杂混合物中污染物的差异鉴定激活了LUC基因的表达。 该测定不需要杀死鱼，并允许用相同的鱼重复分析相同的位点。 可以通过改变响应元件的顺序来操纵系统的灵敏度。

公开(公告)号：US20040206162A1

公开(公告)日：2004-10-21

申请号：US10774639

申请日：2004-02-03

发明人： Tommy R. Shedd ,  Mark W. Widder ,  Mark W. Brown ,  Murray S. Swanson

IPC分类号： G01N033/18

CPC分类号： G01N33/186

摘要： A portable automated biomonitoring system for monitoring water quality includes an exposure chamber for housing an aquatic organism and a water inlet for directing water to the exposure chamber. The aquatic organism has ventilatory behavior and body movement sensitive to water quality. Electrodes sense electrical signals produced by the organism during its ventilatory behavior and body movement, and a controller responsive to signals from the electrodes determines a plurality of ventilatory parameters based on the signals. The ventilatory parameters are compared with corresponding thresholds to determine when the water to which the organism is exposed has caused physiological stress to the organism. The water being evaluated may be recirculated through the system for further analysis.

摘要翻译： 用于监测水质的便携式自动生物监测系统包括用于容纳水生生物的暴露室和用于将水引导到暴露室的进水口。 水生生物具有对水质敏感的通气行为和身体运动。 电极在其通气行为和身体移动期间感测由生物体产生的电信号，并且响应于来自电极的信号的控制器基于该信号确定多个通气参数。 将通气参数与相应的阈值进行比较，以确定生物体暴露的水何时对生物体产生生理压力。 被评估的水可以通过系统再循环以进一步分析。

公开(公告)号：US20020129386A1

公开(公告)日：2002-09-12

申请号：US09893666

申请日：2001-06-29

发明人： Ichiro Yamashita

IPC分类号： A01K067/027 ,  C07H021/04 ,  C12N015/00

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/40 ,  A01K2267/03 ,  C07K14/461 ,  G01N33/186 ,  G01N33/5082 ,  G01N33/74 ,  G01N2520/00

摘要： (1) Transgenic medaka fish into which a polynucleotide having the nucleotide sequence from 211 to 1935 position represented by Sequence ID No: 1 is introduced, (2) A method of producing medaka fish having one or more thrombi, comprising the step of raising the transgenic medaka fish described in (1) in the presence of estrogen, (3) Medaka fish having one or more thrombi produced by the method described in (2), and (4) A method of testing an estrogen-like acting substance, comprising the steps of raising the transgenic medaka fish described in (1) in test water, and observing whether or not one or more thrombi are formed in the medaka fish after the raising step.

摘要翻译： （1）引入具有由序列号1表示的211〜1935位置的碱基序列的多核苷酸的转基因aka鱼，（2）一种制备具有一个或多个血栓的aka鱼的方法，其包括： （1）中描述的在雌激素存在下的转基因aka鱼，（3）通过（2）中描述的方法制备的具有一种或多种血栓的楝树鱼，和（4）一种测试雌激素样作用物质的方法，包括 （1）中描述的转基因aka鱼在试验水中的步骤，并且观察在升高步骤之后是否在aka鱼中形成一个或多个血栓。