公开(公告)号：US20190177412A1

公开(公告)日：2019-06-13

申请号：US16310121

申请日：2017-06-15

申请人： AUTOLUS LIMITED

发明人： Shimobi Onuoha ,  Martin Pulé ,  Simon Thomas ,  Shaun Cordoba ,  Evangelia Kokalaki

IPC分类号： C07K16/28 ,  C07K14/705 ,  A61K35/17 ,  A61P35/00 ,  A61P35/02 ,  C07K14/725

摘要： The present invention provides a chimeric antigen receptor (CAR) which binds human CD22, having an antigen-binding domain which comprises a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences: CDR1—NYWIH (SEQ ID No. 1); CDR2—GINPGNNYATYRRKFQG (SEQ ID No. 2) CDR3—EGYGNYGAWFAY (SEQ ID No. 3); and b) a light chain variable region (VL) having CDRs with the following sequences: CDR1—RSSQSLANSYGNTFLS (SEQ ID No. 4); CDR2—GISNRFS (SEQ ID No. 5) CDR3—LQGTHQPYT (SEQ ID No. 6). The present invention also provides a cell comprising such a CAR and the use of such a cell to treat cancer.

公开(公告)号：US10174099B2

公开(公告)日：2019-01-08

申请号：US15632119

申请日：2017-06-23

申请人： UCL BUSINESS PLC ,  AUTOLUS LIMITED

发明人： Martin Pulé ,  Shaun Cordoba ,  Shimobi Onuoha ,  Simon Thomas

IPC分类号： A61K35/00 ,  A61K35/17 ,  A61K38/17 ,  A61K39/00 ,  C07K16/28 ,  C07K16/30 ,  C07K14/725 ,  C12N5/0783 ,  C07K14/705 ,  C07K14/735

摘要： The present invention provides a cell which co-expresses a first chimeric antigen receptor (CAR) and second CAR at the cell surface, each CAR comprising an antigen-binding domain, wherein the antigen-binding domain of the first CAR binds to CD19 and the antigen-binding domain of the second CAR binds to CD22.

公开(公告)号：US20180305433A1

公开(公告)日：2018-10-25

申请号：US15770130

申请日：2016-10-21

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Shaun Cordoba

IPC分类号： C07K14/725 ,  A61K35/17 ,  C07K16/28 ,  C07K14/705 ,  C12N5/0783 ,  C12N9/16

CPC分类号： C07K14/7051 ,  A61K35/17 ,  A61K39/00 ,  A61K39/0011 ,  A61K2039/5156 ,  A61K2039/5158 ,  C07K14/70517 ,  C07K16/00 ,  C07K16/2803 ,  C07K16/2896 ,  C07K2317/622 ,  C07K2319/03 ,  C07K2319/33 ,  C12N5/0638 ,  C12N9/16 ,  C12N2510/00 ,  C12Y301/03048

摘要： The present invention relates to a cell which co-expresses a first chimeric antigen receptor (CAR) and second CAR at the cell surface, each CAR comprising: (i) an antigen-binding domain; (ii) a spacer (iii) a trans-membrane domain; and (iv) an endodomain wherein the first CAR is an activating CAR comprising an activating endodomain and the second CAR is an inhibitory CAR comprising a ligation-off inhibitory endodomain; wherein the first CAR binds to a first antigen and the second CAR binds to a second antigen, wherein both the first and second antigens are expressed on the surface of a target cell; wherein the first CAR binds to an epitope on the first antigen which is relatively proximal to the membrane of the target cell, the second CAR binds to an epitope on the second antigen which is relatively distal to the membrane of the target cell, in comparison with the epitope of the first antigen; and wherein the spacer of the first CAR is an equivalent size to the spacer of the second CAR.

公开(公告)号：US20240093232A1

公开(公告)日：2024-03-21

申请号：US18160032

申请日：2023-01-26

申请人： AUTOLUS LIMITED

发明人： Vijay Peddareddigari ,  Richard Beswick ,  John Short ,  Martin Pulé ,  Ekaterini Kotsopoulou

IPC分类号： C12N15/86 ,  C07K14/00 ,  C07K14/705 ,  C07K14/725 ,  C07K16/28

CPC分类号： C12N15/86 ,  C07K14/00 ,  C07K14/7051 ,  C07K14/70596 ,  C07K16/2809 ,  C07K16/2818 ,  A61K38/00 ,  C07K2317/622 ,  C12N2740/13032 ,  C12N2740/13045 ,  C12N2740/15032 ,  C12N2740/15045 ,  C12N2740/15052 ,  C12N2810/852 ,  C12N2810/855 ,  C12N2810/859

摘要： The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises a mitogenic T-cell activating transmembrane protein which comprises: (i) a mitogenic domain which binds a mitogenic tetraspanin, and (ii) a transmembrane domain; wherein the mitogenic T-cell activating transmembrane protein is not part of a viral envelope glycoprotein. When cells such as T-cells or Natural Killer cells are transduced by such a viral vector, they are activated by the mitogenic T-cell activating transmembrane protein.

公开(公告)号：US20240033292A1

公开(公告)日：2024-02-01

申请号：US18321517

申请日：2023-05-22

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Khai Kong ,  Shaun Cordoba

IPC分类号： A61K35/17 ,  A61K39/00 ,  C07K14/725 ,  C07K14/705 ,  C07K16/28 ,  C12N5/0783 ,  C12N15/86 ,  C12N9/16

CPC分类号： A61K35/17 ,  A61K39/0011 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70589 ,  C07K16/2803 ,  C12N5/0638 ,  C12N5/0646 ,  C07K14/70503 ,  C12N5/0636 ,  C12N15/86 ,  C12N5/0637 ,  C12N9/16 ,  C12Y301/03048 ,  A61K2039/5158 ,  C12N2510/00 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2319/01 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2740/10043 ,  C12N2740/15043 ,  A61K38/00

摘要： The present invention provides a cell which co-expresses a first chimeric antigen receptor (CAR) and second CAR at the cell surface, each CAR comprising: (i) an antigen-binding domain; (ii) a spacer (iii) a transmembrane domain; and (iv) an endodomain wherein the antigen binding domains of the first and second CARs bind to different antigens, wherein the spacer of the first CAR is different to the spacer of the second CAR and wherein one of the first or second CARs is an activating CAR comprising an activating endodomain and the other CAR is an inhibitory CAR comprising a ligation-off inhibitory endodomain.

公开(公告)号：US20240033289A1

公开(公告)日：2024-02-01

申请号：US18158310

申请日：2023-01-23

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Khai Kong ,  Shaun Cordoba

IPC分类号： A61K35/17 ,  A61K39/00 ,  C07K14/725 ,  C07K14/705 ,  C07K16/28 ,  C12N5/0783 ,  C12N15/86 ,  C12N9/16

CPC分类号： A61K35/17 ,  A61K38/00 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70589 ,  C07K16/2803 ,  C12N5/0638 ,  C12N5/0646 ,  C07K14/70503 ,  C12N5/0636 ,  C12N15/86 ,  C12N5/0637 ,  C12N9/16 ,  C12Y301/03048 ,  A61K2039/5158 ,  C12N2510/00 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2319/01 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2740/10043 ,  C12N2740/15043 ,  A61K39/0011

摘要： The present invention provides a cell which co-expresses a first chimeric antigen receptor (CAR) and second CAR at the cell surface, each CAR comprising: (i) an antigen-binding domain; (ii) a spacer (iii) a trans-membrane domain; and (iv) an endodomain wherein the antigen binding domains of the first and second CARs bind to different antigens, and wherein each of the first and second CARs is an activating CAR comprising an activating endodomain.

公开(公告)号：US11879016B2

公开(公告)日：2024-01-23

申请号：US17196670

申请日：2021-03-09

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  John Anderson ,  Simon Thomas

IPC分类号： C07K16/30 ,  C07K14/725 ,  C07K14/705 ,  A61K35/17 ,  A61K38/17 ,  A61K39/395 ,  C12N15/85 ,  A61K39/00

CPC分类号： C07K16/3084 ,  A61K35/17 ,  A61K38/179 ,  A61K38/1774 ,  A61K39/39558 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70578 ,  C12N15/85 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/53 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/20 ,  C07K2319/30 ,  C12N2510/00

摘要： Provision of a chimeric antigen receptor (CAR) comprising a disialoganglioside (GD2)-binding domain which comprises •a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences: •b) a light chain variable region (VL) having CDRs with the following sequences: T cells expressing such a CAR are useful in the treatment of some cancers.

公开(公告)号：US11851672B2

公开(公告)日：2023-12-26

申请号：US16759222

申请日：2018-10-24

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  James Sillibourne ,  Shaun Cordoba

IPC分类号： C12N15/86 ,  A61K35/17 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K35/17 ,  A61K48/00 ,  C12N2740/10043 ,  C12N2800/40

摘要： The present invention provides a kit of vectors for transducing an immune cell with multiple transgenes comprising: (i) a first vector which comprises a first transgene and a nucleotide sequence encoding a transcription factor and; and (ii) a second vector which comprises a second transgene wherein expression of the second transgene within a host cell is dependent upon expression of the transcription factor.

公开(公告)号：US20230346838A1

公开(公告)日：2023-11-02

申请号：US18191163

申请日：2023-03-28

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Anne Kramer ,  Evangelia K. Kokalaki

IPC分类号： A61K35/17 ,  C07K14/705 ,  C07K14/725 ,  C07K16/28

CPC分类号： A61K35/17 ,  C07K14/70503 ,  C07K14/7051 ,  C07K16/2803 ,  C07K14/70578 ,  C07K14/70517 ,  C07K2317/622 ,  C07K2319/33 ,  C07K2319/03 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/74

摘要： The present invention provides a chimeric antigen receptor (CAR) comprising an antigen-binding domain with an affinity in the range of 50 nM to 500 nM, wherein said affinity comprises component kinetics such that the association rate constant (kon) is greater than or equal to 1×105 M−1 S−1, and/or the dissociation rate constant (koff) is greater than or equal to 0.01 s−1.

公开(公告)号：US20230330139A1

公开(公告)日：2023-10-19

申请号：US17268328

申请日：2019-08-13

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Shaun Cordoba ,  Simon Thomas ,  Shimobi Onuoha ,  Alexander Kinna ,  Mathieu Ferrari

IPC分类号： A61K35/17 ,  C07K16/28 ,  A61P35/02

CPC分类号： A61K35/17 ,  C07K16/2803 ,  C07K16/2851 ,  C07K16/2866 ,  A61P35/02

摘要： The present disclosure provides a cell comprising: an anti-CD33 chimeric antigen receptor (CAR); an anti-CLL1 CAR; and an anti- CD123 and/or anti- CAR FLT3 CAR. The cell can be used in the treatment of a disease such as acute myeloid leukemia (AML).