公开(公告)号：US20210207178A1

公开(公告)日：2021-07-08

申请号：US16994240

申请日：2020-08-14

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard

IPC: C12P7/64 ,  C12P7/04 ,  C12P7/24 ,  C12N15/52 ,  C07C31/20 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/88 ,  C12N9/00

Abstract: The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.

公开(公告)号：US20210147883A1

公开(公告)日：2021-05-20

申请号：US16785510

申请日：2020-02-07

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Priti Pharkya ,  Tae Hoon Yang ,  Jungik Choi

IPC: C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N15/52 ,  C12N1/20 ,  C12N9/00 ,  C07K14/245 ,  C12P7/42 ,  C12N9/52 ,  C12N9/88

Abstract: The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

公开(公告)号：US20210108232A1

公开(公告)日：2021-04-15

申请号：US16884976

申请日：2020-05-27

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Anthony P. Burgard ,  Priti Pharkya

IPC: C12P7/04 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P7/02 ,  C12N15/52

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：US20210040511A1

公开(公告)日：2021-02-11

申请号：US16997704

申请日：2020-08-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/42 ,  C12P7/40 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90

Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

公开(公告)号：US10662451B2

公开(公告)日：2020-05-26

申请号：US15971878

申请日：2018-05-04

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Wei Niu ,  John D. Trawick ,  Robert Haselbeck

IPC: C12N1/20 ,  C12N1/14 ,  C12P17/04 ,  C12P7/18 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

公开(公告)号：US20190330670A1

公开(公告)日：2019-10-31

申请号：US16213671

申请日：2018-12-07

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12P13/02 ,  C12P13/00 ,  C12P17/12 ,  C12P7/40 ,  C12N15/52 ,  C12N1/38 ,  C12P7/44

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US20190284592A1

公开(公告)日：2019-09-19

申请号：US16433804

申请日：2019-06-06

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya ,  Mark J. Burk

IPC: C12P17/08 ,  C12N15/52 ,  C12N5/00 ,  C08G63/08 ,  C07D313/04 ,  C12P17/02 ,  C12N15/70 ,  C12N15/63 ,  C08G18/42

Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

公开(公告)号：US10273508B2

公开(公告)日：2019-04-30

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. Van Dien ,  Anthony P. Burgard ,  Robert Haselbeck ,  Catherine J. Pujol-Baxley ,  Wei Niu ,  John D. Trawick ,  Harry Yim ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

IPC: C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

公开(公告)号：US20190062758A1

公开(公告)日：2019-02-28

申请号：US15925301

申请日：2018-03-19

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

IPC: C12N15/52 ,  B01D3/00 ,  C12N15/70 ,  C12P17/04 ,  C12P7/52 ,  C12P7/42 ,  C12P7/18 ,  C12N9/00 ,  C12N9/88 ,  C12N9/02 ,  C12N9/04 ,  C12N15/81

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.

公开(公告)号：US20190032095A1

公开(公告)日：2019-01-31

申请号：US16041271

申请日：2018-07-20

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk

IPC: C12P7/44 ,  C12P7/24 ,  C12N9/10 ,  C12P9/00 ,  C12N9/02 ,  C07C51/43 ,  C12N9/12 ,  C08G63/183 ,  C12N9/88 ,  C12N9/04 ,  C12P7/62 ,  C07F9/09 ,  C12P7/40 ,  C07C63/26 ,  C07C63/04

Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate (2H3M4OP) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M4OP, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.