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公开(公告)号：US11365437B2
公开(公告)日：2022-06-21
申请号：US15936293
申请日：2018-03-26
Applicant: Translate Bio, Inc.
Inventor： Michael Heartlein , Frank DeRosa , Anusha Dias
IPC: C12Q1/68 , C12Q1/6823 , C07K14/505 , C12N9/02
Abstract: The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly for mRNA synthesized in vitro. In some embodiments, the methods comprise chromatographic methods of quantifying capping efficiency and methylation status of the caps.
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公开(公告)号：US20210145860A1
公开(公告)日：2021-05-20
申请号：US17076195
申请日：2020-10-21
Applicant: Translate Bio, Inc.
Inventor： Richard Wooster , Anusha Dias , Dustin Cooper , Christian Cobaugh , Frank DeRosa , Tim Efthymiou , Jeffrey S. Dubins
IPC: A61K31/7105 , A61K47/54 , A61K47/60 , A61K47/69
Abstract: The present invention provides, among other things, methods and compositions for selective degradation of proteins. In some aspects, messenger RNAs (mRNAs) are described that encode a ubiquitin pathway moiety and a binding peptide that binds a target protein, wherein the mRNA is encapsulated within a lipid nanoparticle. Also provided herein are mRNAs that encode at least two binding peptides, wherein a first binding peptide binds a ubiquitin pathway moiety and a second binding peptide binds a target protein, and wherein the mRNA is encapsulated within a lipid nanoparticle.
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公开(公告)号：US10808241B2
公开(公告)日：2020-10-20
申请号：US15906864
申请日：2018-02-27
Applicant: Translate Bio, Inc.
Inventor： Jonathan Abysalh , Daniel Crawford , Frank DeRosa , Shrirang Karve , Anusha Dias , Michael Heartlein
IPC: C12N15/10 , B01L3/00 , C12Q1/6806
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
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公开(公告)号：US20180258423A1
公开(公告)日：2018-09-13
申请号：US15907163
申请日：2018-02-27
Applicant: Translate Bio, Inc.
Inventor： Anusha Dias , Daniel Crawford , Frank DeRosa , Jonathan Abysalh , Michael Heartlein
IPC: C12N15/10 , G01N27/447
CPC classification number: C12N15/1096 , C12N9/1247 , C12N15/1068 , C12P19/34 , C12Y207/07006 , G01N27/44778
Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.
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Patent Agency Ranking
公开(公告)号：US20180256741A1
公开(公告)日：2018-09-13
申请号：US15907131
申请日：2018-02-27
Applicant: Translate Bio, Inc.
Inventor： Anusha Dias , Jonathan Abysalh , Brian Bettencourt , Frank DeRosa , Michael Heartlein
Abstract: The present invention provides, among other things, improved methods and pharmaceutical compositions for treating cystic fibrosis based on codon optimized mRNA encoding a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein.
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公开(公告)号：US20180251755A1
公开(公告)日：2018-09-06
申请号：US15906864
申请日：2018-02-27
Applicant: Translate Bio, Inc.
Inventor： Jonathan Abysalh , Daniel Crawford , Frank DeRosa , Shrirang Karve , Anusha Dias , Michael Heartlein
IPC: C12N15/10 , C12Q1/6806 , B01L3/00
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
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公开(公告)号：US20180251754A1
公开(公告)日：2018-09-06
申请号：US15907086
申请日：2018-02-27
Applicant: Translate Bio, Inc.
Inventor： Frank DeRosa , Michael Heartlein , Jonathan Abysalh , Daniel Crawford , Anusha Dias , Shrirang Karve
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
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公开(公告)号：US20180237766A1
公开(公告)日：2018-08-23
申请号：US15936289
申请日：2018-03-26
Applicant: Translate Bio, Inc.
Inventor： Michael Heartlein , Frank DeRosa , Anusha Dias , Shrirang Karve
CPC classification number: C12N15/10 , C07H21/02 , C12N15/1017
Abstract: The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized rnRNA to a denaturing condition, and purifying the rnRNA from the impure preparation from step (a) by tangential flow filtration, wherein the mRNA purified from step (b) substantially free of prematurely aborted RNA sequences and/or enzyme reagents used in in vitro synthesis.
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公开(公告)号：US09970047B2
公开(公告)日：2018-05-15
申请号：US14775846
申请日：2014-03-14
Applicant: TRANSLATE BIO, INC.
Inventor： Michael Heartlein , Frank DeRosa , Anusha Dias
IPC: C12Q1/68 , C07K14/50 , C12N9/00 , C07K14/505 , C12N9/02
CPC classification number: C12Q1/68 , C07K14/505 , C12N9/0069 , C12Q1/6823 , C12Y113/12007 , C12Q2521/327 , C12Q2525/186 , C12Q2537/113 , C12Q2565/137
Abstract: The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly for mRNA synthesized in vitro. In some embodiments, the methods comprise chromatographic methods of quantifying capping efficiency and methylation status of the caps.
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