公开(公告)号：US08268626B2

公开(公告)日：2012-09-18

申请号：US11791327

申请日：2005-11-24

申请人： Mitsuyo Okazaki

发明人： Mitsuyo Okazaki

IPC分类号： G01N30/86 ,  G01N30/00

CPC分类号： G01N30/8675 ,  G01N30/8679 ,  G01N33/92 ,  G01N2030/8813 ,  G01N2030/8831

摘要： A method of analysis of lipoproteins according to the present invention comprises the steps of: separating a plurality of classes of lipoproteins contained in a subject sample by liquid chromatography and then detecting signals derived from components included in the separated lipoproteins; and assuming that the lipoproteins are constituted of subclasses estimated from component peaks comprising anchor peaks and extra essential peaks and then calculating an approximated curve corresponding to summation of the component peaks using the above described detected signals.

摘要翻译： 根据本发明的脂蛋白的分析方法包括以下步骤：通过液相色谱法分离包含在受试者样品中的多种类型的脂蛋白，然后检测源自包含在分离的脂蛋白中的组分的信号; 并且假定脂蛋白由从包括锚定峰和额外必需峰的组分峰估计的亚类构成，然后使用上述检测到的信号计算对应于分量峰的总和的近似曲线。

公开(公告)号：US08143066B2

公开(公告)日：2012-03-27

申请号：US11916320

申请日：2006-06-05

申请人： Marc V. Gorenstein ,  Scott J. Geromanos ,  Jeffrey Cruz Silva ,  Guo-Zhong Li

发明人： Marc V. Gorenstein ,  Scott J. Geromanos ,  Jeffrey Cruz Silva ,  Guo-Zhong Li

IPC分类号： G01N33/00

CPC分类号： H01J49/04 ,  C12Q1/37 ,  G01N27/62 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/8665 ,  G01N30/8675 ,  G01N30/8679 ,  G01N33/6848 ,  G01N2030/042 ,  G01N2030/067

摘要： A method (100) for analyzing chemicals includes fractionating a complex sample into at least two sample portions that each includes portions of two polypeptides though in different concentration ratios, digesting and performing LC/MS on each of the sample portions (110, and associating precursor ions observed via LC/MS with their corresponding polypeptide in response to LC/MS provided intensity data (170). A set of precursor ions that has substantially similar intensity ratios in both sample portions is determined to be associated with the same polypeptide.

摘要翻译： 用于分析化学品的方法（100）包括将复杂样品分成至少两个样品部分，每个样品部分各自包含不同浓度比的两种多肽的部分，在每个样品部分（110和缔合前体）上消化并进行LC / MS 通过LC / MS与其对应的多肽响应于LC / MS观察到的离子提供强度数据（170）。确定两组样品部分中具有基本上相似的强度比的一组前体离子与相同的多肽相关。

公开(公告)号：US20110091984A1

公开(公告)日：2011-04-21

申请号：US12994251

申请日：2009-05-21

申请人： Mark D. Williams ,  James Slightom ,  Frank Roerink

发明人： Mark D. Williams ,  James Slightom ,  Frank Roerink

IPC分类号： G01N30/02

CPC分类号： G01N30/88 ,  G01N21/6428 ,  G01N30/74 ,  G01N30/8679 ,  G01N2030/027 ,  G01N2030/045 ,  G01N2030/8831

摘要： The present invention is directed to methods of evaluating whether an antigen or vaccine has degraded over time using liquid chromatography. The methods described herein also relate to using liquid chromatography to evaluate the relative potency of a given antigen or vaccine.

摘要翻译： 本发明涉及评估抗原或疫苗是否随时间流逝使用液相色谱法降解的方法。 本文所述的方法还涉及使用液相色谱来评估给定抗原或疫苗的相对效价。

公开(公告)号：US20100100335A1

公开(公告)日：2010-04-22

申请号：US12444670

申请日：2006-10-31

申请人： Haruhiko Miyagawa

发明人： Haruhiko Miyagawa

IPC分类号： G01D18/00 ,  G06F19/00

CPC分类号： G01N30/7206 ,  G01N30/8679 ,  G01N2030/045

摘要： A method for quantitating a substance to be measured according to which a substance to be measured is quantitated is characterized by including: a database storing step of storing a database with which a relative calibration curve is registered; an internal standard substance measuring step of measuring an internal standard substance having a known concentration so that a mass spectrum of the internal standard substance is gained; an absolute value calculating step of calculating the peak area or the height of an internal standard substance by preparing a chromatogram on the basis of the mass spectrum of the internal standard substance; a substance to be measured measuring step of gaining a mass spectrum of the substance to be measured by measuring the substance to be measured; a measured value calculating step of calculating the peak area or the height of the substance to be measured by preparing a chromatogram on the basis of the mass spectrum of the substance to be measured; and a concentration of a substance to be measured calculating step of calculating the concentration of the substance to be measured on the basis of the relative calibration curve, the peak area or the height of the internal standard substance and the peak area or the height of the substance to be measured.

摘要翻译： 定量待测量物质的待测量物质的定量方法的特征在于包括：数据库存储步骤，用于存储登记有相对校准曲线的数据库; 测定具有已知浓度的内标物质，从而获得内标物质的质谱的内标物质测定工序; 绝对值计算步骤，通过基于内标物质的质谱制备色谱图来计算内标物质的峰面积或高度; 待测物质的测定步骤，通过测定待测物质来测定待测物质的质谱; 测量值计算步骤，基于待测物质的质谱，通过制备色谱图来计算待测物质的峰面积或高度; 和待测物质的浓度计算步骤，根据相对校正曲线，内标物质的峰面积或高度以及峰面积或峰高度计算待测物质浓度的计算步骤 待测物质

公开(公告)号：US20090317791A1

公开(公告)日：2009-12-24

申请号：US11916320

申请日：2006-06-05

申请人： Marc V. Gorenstein ,  Scott J. Geromanos ,  Jeffrey Cruz Silva ,  Guo-Zhong Li

发明人： Marc V. Gorenstein ,  Scott J. Geromanos ,  Jeffrey Cruz Silva ,  Guo-Zhong Li

IPC分类号： C12Q1/00

CPC分类号： H01J49/04 ,  C12Q1/37 ,  G01N27/62 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/8665 ,  G01N30/8675 ,  G01N30/8679 ,  G01N33/6848 ,  G01N2030/042 ,  G01N2030/067

摘要： A method (100) for analyzing chemicals includes fractionating a complex sample into at least two sample portions that each includes potions of two polypeptides though in different concentration ratios, digesting and performing LC/MS on each of the sample portions (110, and associating precursor ions observed via LC/MS with their corresponding polypeptide in response to LC/MS provided intensity data (170). A set of precursor ions that has substantially similar intensity ratios in both sample portions is determined to be associated with the same polypeptide.

摘要翻译： 用于分析化学品的方法（100）包括将复杂样品分馏成至少两个样品部分，每个样品部分各自包含不同浓度比的两种多肽，在每个样品部分（110和缔合前体）上消化并进行LC / MS 通过LC / MS与其对应的多肽响应于LC / MS观察到的离子提供强度数据（170）。确定两组样品部分中具有基本上相似的强度比的一组前体离子与相同的多肽相关。

公开(公告)号：US20090001261A1

公开(公告)日：2009-01-01

申请号：US12136381

申请日：2008-06-10

申请人： Shinichi YAMAGUCHI

发明人： Shinichi YAMAGUCHI

IPC分类号： H01J49/26

CPC分类号： H01J49/0036 ,  G01D7/02 ,  G01N30/72 ,  G01N30/86 ,  G01N30/8675 ,  G01N30/8679 ,  G16C20/20 ,  G16C20/80

摘要： A chromatograph mass analysis data processing apparatus is provided for obtaining pertinent information, with a simple operation, on a compound series including a plurality of compounds whose structures and characters are similar. Based on the data obtained by a chromatograph mass analysis, a two-dimensional isointensity line graph is created and displayed with a retention time and a mass-to-charge ratio on the two axes and with a signal intensity represented with a contour (S1). When the operator specifies a desired range by a drag operation or the like through a mouse (S2), the data contained in the range specified are collected (S3), the signal intensities along the mass-to-charge ratio axis direction are summed up for every retention time to create a summed mass chromatogram based on the summed value (S4 and S5). Simultaneously, the signal intensities along the retention time axis direction are summed up for every mass-to-charge ratio to create a summed mass spectrum based on the summed value (S6 and S7). These are then displayed on the same screen of the isointensity line graph (S8).

摘要翻译： 提供了一种色谱质量分析数据处理装置，用于通过简单的操作获得关于包括其结构和特征相似的多种化合物的化合物系列的相关信息。 基于通过色谱质量分析获得的数据，创建并显示二维等距线图，其中在两轴上具有保持时间和质荷比，并且以轮廓（S1）表示的信号强度， 。 当操作者通过鼠标拖动操作等来指定期望的范围（S2）时，收集指定范围中包含的数据（S3），沿着质荷比轴方向的信号强度相加 每个保留时间基于总和值创建总和质谱图（S4和S5）。 同时，沿着保持时间轴方向的信号强度针对每个质荷比进行相加，以基于求和值产生求和质谱（S6和S7）。 然后将它们显示在等速线图的同一屏幕上（S8）。

公开(公告)号：US20070246427A1

公开(公告)日：2007-10-25

申请号：US11809067

申请日：2007-05-31

申请人： Jose Castro-Perez ,  Robert Plumb ,  Jane Kirby

发明人： Jose Castro-Perez ,  Robert Plumb ,  Jane Kirby

IPC分类号： B01D15/08

CPC分类号： G01N30/8675 ,  G01N30/8617 ,  G01N30/8679

摘要： A method of rapidly analyzing unexpected metabolites in a metabolite analyzing system is disclosed. A control sample is run and analyzed to generate an exclusion list of unwanted sample components. A single analyte sample is then run and programmatically uses the exclusion list containing the unwanted metabolites to dynamically filter out data regarding components present in both the control sample and the analyte sample. The remaining components in the analyte sample are analyzed for unexpected metabolites of interest. The present invention allows for the analysis to be automated and eliminates the need for a second analyte sample run for the purpose of eliminating common components in the samples.

摘要翻译： 公开了一种在代谢分析系统中快速分析意外代谢物的方法。 运行和分析对照样品以产生不需要的样品组分的排除列表。 然后运行单个分析物样品，并以编程方式使用包含不需要的代谢物的排除列表，以动态过滤关于对照样品和分析物样品中存在的成分的数据。 分析分析物样品中的剩余组分，以获得意想不到的感兴趣的代谢物。 本发明允许分析自动化，并且消除了为了消除样品中的常见组分的目的而需要第二分析物样品运行。

公开(公告)号：US07117103B2

公开(公告)日：2006-10-03

申请号：US10859843

申请日：2004-06-03

申请人： Michael Joseph Szelewski ,  Elmer Anthony Axelson ,  Chin-Kai Meng

发明人： Michael Joseph Szelewski ,  Elmer Anthony Axelson ,  Chin-Kai Meng

IPC分类号： G06F19/00

CPC分类号： G01N30/8675 ,  G01N30/8679

摘要： A method for automatically verifying the existence of a target compound comprises generating a total ion chromatogram. The total ion chromatogram comprises a plurality of peaks, each peak representing one or more compounds in a sample matrix, each peak comprising at least two compounds. The method also comprises deconvoluting each peak to isolate each target compound present in the peak, and automatically verifying the identity of each target compound against a target compound library.

摘要翻译： 用于自动验证目标化合物的存在的方法包括产生总离子色谱图。 总离子色谱图包括多个峰，每个峰表示样品基质中的一种或多种化合物，每个峰包含至少两种化合物。 该方法还包括对每个峰进行去卷积以分离存在于峰中的每个目标化合物，并自动验证每个目标化合物与目标化合物文库的同一性。

公开(公告)号：US20050273276A1

公开(公告)日：2005-12-08

申请号：US10859843

申请日：2004-06-03

申请人： Michael Joseph Szelewski ,  Elmer Anthony Axelson ,  Chin-Kai Meng

发明人： Michael Joseph Szelewski ,  Elmer Anthony Axelson ,  Chin-Kai Meng

IPC分类号： G01N30/86 ,  G06F19/00

CPC分类号： G01N30/8675 ,  G01N30/8679

摘要： A method for automatically verifying the existence of a target compound comprises generating a total ion chromatogram. The total ion chromatogram comprises a plurality of peaks, each peak representing one or more compounds in a sample matrix, each peak comprising at least two compounds. The method also comprises deconvoluting each peak to isolate each target compound present in the peak, and automatically verifying the identity of each target compound against a target compound library.

摘要翻译： 用于自动验证目标化合物的存在的方法包括产生总离子色谱图。 总离子色谱图包括多个峰，每个峰表示样品基质中的一种或多种化合物，每个峰包含至少两种化合物。 该方法还包括对每个峰进行去卷积以分离存在于峰中的每个目标化合物，并自动验证每个目标化合物与目标化合物文库的同一性。

公开(公告)号：US06873914B2

公开(公告)日：2005-03-29

申请号：US10300360

申请日：2002-11-20

申请人： Stephanie Winfield ,  Norman Glassbrook ,  Yasmin Ranasinghe ,  David Broadwell ,  Ioana Popa-Burke ,  Gordon James Nye ,  Christopher Beecher

发明人： Stephanie Winfield ,  Norman Glassbrook ,  Yasmin Ranasinghe ,  David Broadwell ,  Ioana Popa-Burke ,  Gordon James Nye ,  Christopher Beecher

IPC分类号： G01N30/72 ,  G01N30/86 ,  G01N31/00 ,  G01N33/48 ,  G06F19/12 ,  G06F19/28 ,  G06G7/48

CPC分类号： G06F19/12 ,  G01N30/72 ,  G01N30/8655 ,  G01N30/8658 ,  G01N30/8662 ,  G01N30/8679 ,  G01N30/8693 ,  G06F19/28 ,  Y10S707/99943

摘要： The present invention provides methods and systems for organizing complex and disparate data. More specifically, the present invention provides methods and systems for organizing complex and disparate data into coherent data sets. Coherent data sets resulting from the methods and systems of the present invention serve as models for biological systems. Methods and systems for integrating data and creating coherent data sets are useful for numerous biological applications, such as, for example, determining gene function, identifying and validating drug and pesticide targets, identifying and validating drug and pesticide candidate compounds, profiling drug and pesticide compounds, producing a compilation of health or wellness profiles, determining compound site(s) of action, identifying unknown samples, and numerous other applications in the agricultural, pharmaceutical, forensic, and biotechnology industries.

摘要翻译： 本发明提供了用于组织复杂且不同的数据的方法和系统。 更具体地，本发明提供了将复杂且不同的数据组织成相干数据集的方法和系统。 由本发明的方法和系统产生的相干数据集用作生物系统的模型。 用于整合数据和创建连贯数据集的方法和系统对于许多生物学应用是有用的，例如确定基因功能，识别和验证药物和农药目标，鉴定和验证药物和农药候选化合物，分析药物和农药化合物 编制健康或健康档案汇编，确定复合的行动地点，识别未知样本以及农业，制药，法医和生物技术行业的众多其他应用。