公开(公告)号：US20230144767A1

公开(公告)日：2023-05-11

申请号：US18093704

申请日：2023-01-05

申请人： University of Pittsburgh - Of the Commonwealth System of Higher Education

发明人： Peter Wipf ,  Marie-Celine Frantz

IPC分类号： C07K5/08 ,  A61K31/454 ,  C07D211/58 ,  C07D401/12 ,  A61K47/64 ,  C07D417/12 ,  C07C237/16 ,  C07D209/46 ,  C07D211/94 ,  C07D221/22 ,  C07D209/44 ,  C07D279/06 ,  C07D471/08

CPC分类号： C07K5/08 ,  A61K31/454 ,  C07D211/58 ,  C07D401/12 ,  A61K47/64 ,  C07D417/12 ,  C07C237/16 ,  C07D209/46 ,  C07D211/94 ,  C07D221/22 ,  C07D209/44 ,  C07D279/06 ,  C07D471/08

摘要： Provided herein are compositions and related methods useful for free radical scavenging, with particular selectivity for mitochondria. The compounds comprise a nitroxide-containing group attached to a mitochondria-targeting group. The compounds can be cross-linked into dimers without loss of activity. Also provided herein are methods, for preventing, mitigating and treating damage caused by radiation. The method comprises delivering a compound, as described herein, to a patient in an amount and dosage regimen effective to prevent, mitigate or treat damage caused by radiation.

公开(公告)号：US11634716B2

公开(公告)日：2023-04-25

申请号：US16755717

申请日：2018-10-15

申请人： University of Pittsburgh—Of the Commonwealth System of Higher Education

发明人： Cynthia St. Hilaire

IPC分类号： C12N15/113 ,  C12N5/0775 ,  A61L27/38 ,  C12N15/86

摘要： A cardiovascular graft is provided, such as a prosthetic heart valve or blood vessel, that comprises mesenchymal stem cells (MSCs) and/or progeny thereof, modified to knock down or knockout expression of the telomerases reverse transcriptase gene (TERT), or otherwise reduce activity of TERT. Also provided are a method of making the cardiovascular graft, and a method of implanting the cardiovascular graft.

公开(公告)号：US11633464B2

公开(公告)日：2023-04-25

申请号：US16688930

申请日：2019-11-19

申请人： University of Pittsburgh—Of the Commonwealth System of Higher Education

发明人： Nikola L. Vujanovic ,  Lazar Vujanovic

IPC分类号： G01N31/00 ,  G01N33/53 ,  A61K39/00 ,  A61P35/00 ,  A61K38/20 ,  C07K14/525 ,  A61K45/06

摘要： Methods are disclosed for inhibiting the development of a tumor in a subject. The methods include administering to a subject a therapeutically effective amount of a dominant negative tumor necrosis factor (DN-TNF)-α protein and/or a nucleic acid encoding the DN-TNF-α protein. The DN-TNF-α protein and/or a nucleic acid encoding the DN-TNF-α protein can be administered alone or in combination with other agents.

公开(公告)号：US20230117388A1

公开(公告)日：2023-04-20

申请号：US17966444

申请日：2022-10-14

申请人： University of Pittsburgh - Of the Commonwealth System of Higher Education

发明人： Michael T. Lotze ,  Pranav Murthy

IPC分类号： A61K35/17 ,  C12N5/0783 ,  A61P35/00

摘要： This document provides methods and materials for expanding tumor infiltrating γδ T cells (e.g., tumor infiltrating γδ T cells) in culture. For example, methods and materials for expanding large numbers of tumor infiltrating γδ T cells (e.g., tumor infiltrating γδ T cells that are predominantly Vδ1+) from tissue obtained from a mammal having cancer (e.g., a tumor sample), an autoimmune condition, or an infection are provided. Populations of such tumor infiltrating γδ T cells and methods and materials for using such tumor infiltrating γδ T cells and/or such populations to treat cancer within a mammal (e.g., a human) also are provided.

公开(公告)号：US11629370B2

公开(公告)日：2023-04-18

申请号：US17202886

申请日：2021-03-16

申请人： University of Pittsburgh - Of the Commonwealth System of Higher Education ,  Duquesne University of the Holy Spirit

发明人： John Alston Kellum ,  John D. Hempel ,  Robert Hugh Edgar ,  John Andrew Viator

IPC分类号： C12Q1/04 ,  G01N29/24 ,  G01N15/10 ,  C12N7/00 ,  C12N7/02

摘要： A method of detecting a species, strain or type of bacteria includes mixing a labeled bacteriophage including a label that is detectible via a detection system with a bacterial culture including the species, strain or type of bacteria to which the labeled bacteriophage selectively binds and using the detection system to detect the labeled bacteriophage bound to the species, strain or type of bacteria.

公开(公告)号：US20230076977A1

公开(公告)日：2023-03-09

申请号：US17792707

申请日：2021-01-14

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  University of Pittsburgh - Of the Commonwealth System of Higher Education

发明人： Tejvir S. KHURANA ,  Donna M. HURYN

IPC分类号： A61K31/165 ,  A61K31/18 ,  A61K31/343 ,  A61K31/4045 ,  A61K31/4184 ,  A61K31/423 ,  A61K31/44 ,  A61K31/519

摘要： The present invention provides methods for increasing expression of utrophin in a subject in need thereof, the method comprising administering to the subject a pharmaceutical composition comprising a post-transcriptionally utrophin upregulator compound. The present invention also provides methods for treating a subject having a muscular dystrophy. This invention further provides methods for high-throughput screening for a post-transcriptional utrophin upregulator compound.

公开(公告)号：US20230065014A1

公开(公告)日：2023-03-02

申请号：US17792653

申请日：2021-01-14

申请人： University of Pittsburgh-Of the Commonwealth System of Higher Education

发明人： Gary Yu ,  Bruce A. Freeman ,  Flordeliza S. Villanueva ,  John J. Pacella ,  Marco Fazzari ,  Xucai Chen ,  Thiruganesh Ramasamy

IPC分类号： A61K9/50 ,  A61K47/10 ,  A61K47/24 ,  A61K31/19 ,  A61P9/10

摘要： Provided herein are microbubble compositions comprising nitro-fatty acids and/or esters thereof, such as amphiphilic esters or allyl esters thereof. Also provided are methods of reducing local inflammation at a site in a patient comprising delivering the microbubbles to a site of inflammation in the patient and applying ultrasound to the microbubbles. The methods may be used to treat fibrosis or cancer.

公开(公告)号：US20230057542A1

公开(公告)日：2023-02-23

申请号：US17623761

申请日：2020-07-06

申请人： University of Pittsburgh - Of the Commonwealth System of Higher Education

发明人： John F. McDyer ,  Iulia-Dana Popescu ,  Akihiko Hoji

IPC分类号： A61K31/675 ,  A61K31/522 ,  A61K31/662 ,  A61P31/20

摘要： This document relates to methods and materials involved in identifying and/or treating mammals having a CMV infection. For example, methods and materials for assessing a mammal having a CMV infection (e.g., a CMV seronegative human who received CMV seropositive donor transplant tissue) to determine if the mammal is likely to control the CMV infection or to determine if the mammal is unlikely to control the CMV infection are provided. Methods and materials for treating a mammal having a CMV infection (e.g., a CMV seronegative human who received CMV seropositive donor transplant tissue) that was either identified as being likely to control the CMV infection or identified as being unlikely to control the CMV infection also are provided.

公开(公告)号：US11567074B2

公开(公告)日：2023-01-31

申请号：US16336079

申请日：2017-09-25

申请人： University of Pittsburgh—Of the Commonwealth System of Higher Education

发明人： Nathan A. Yates ,  Steven James Mullett ,  Harris B. Bell-Temin ,  Andrey Bondarenko

IPC分类号： G01N33/566 ,  G01N33/68 ,  G01N33/94 ,  G01N33/53 ,  G16B20/00 ,  G01N30/88

摘要： Provided herein are methods of identifying a protein capable of binding a ligand, the method comprising: (a) contacting the ligand with two or more samples comprising a plurality of proteins in a solution; (b) separating the proteins bound to the ligand (“bound proteins”) from the proteins that are not bound to the ligand (“unbound proteins”) in each sample; (c) denaturing and digesting the bound proteins to form a plurality of peptides in each sample; (d) quantifying a plurality of molecular features contained in the plurality of peptides in each sample, wherein the molecular features are defined as having a mass to charge ratio, retention time, and peak intensity as measured by mass spectrometry; and (e) ranking the molecular features that exhibit a statistically significant difference in quantity between the samples contacted with the ligand and a sample that is not contacted with the ligand (“statistically significant molecular feature”).

公开(公告)号：US20230018417A1

公开(公告)日：2023-01-19

申请号：US17623503

申请日：2020-07-24

申请人： Shire Human Genetic Therapies, Inc. ,  University of Pittsburgh-Of The Commonwealth System of Higher Education

发明人： Solomon Fiifi Ofori-Acquah ,  Samit Ghosh ,  Joseph Sypek ,  Bohong Zhang ,  Xiuxia Sun ,  Clark Q. Pan ,  Daniel Minard Lajole ,  Chuan Shen

IPC分类号： A61K38/44 ,  A61P7/06 ,  C12N15/62 ,  C12N9/02

摘要： The present invention provides, among other things, methods and compositions for making and using recombinant heme oxygenase for treating sickle cell disease. In some embodiments, recombinant heme oxygenase proteins are truncation variants, or Fc fusion proteins with increased half-life and/or reduced aggregation.