公开(公告)号：US07368262B2

公开(公告)日：2008-05-06

申请号：US10716793

申请日：2003-11-18

申请人： Debbie Yaver ,  Peter Nham

发明人： Debbie Yaver ,  Peter Nham

IPC分类号： C12N15/00 ,  C12N1/00 ,  C12N15/63 ,  C12Q1/68 ,  C12N1/20

CPC分类号： C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

公开(公告)号：US20050074844A1

公开(公告)日：2005-04-07

申请号：US10656055

申请日：2003-09-05

申请人： Debbie Yaver ,  Alan Sloma

发明人： Debbie Yaver ,  Alan Sloma

IPC分类号： A23J3/34 ,  C12N1/20 ,  C12P21/06 ,  C12Q1/18 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  A23J3/343 ,  C12Q1/18 ,  C12Q1/689 ,  C12Q2600/136 ,  C12Q2600/158

摘要： The present invention relates to arrays for bacterial gene expression profiling in response to treatment with antimicrobial compounds. Bacterial nucleic acid molecules are used as the hybridizable elements in various applications, such as arrays for gene expression profiling and for determining a mode of action for an antimicrobial compound. The present invention also relates to nucleic acid sequences which can be developed as targets for drug screening.

摘要翻译： 本发明涉及用抗菌化合物处理的细菌基因表达谱的阵列。 细菌核酸分子用作各种应用中的可杂交元件，例如用于基因表达谱的阵列和用于确定抗微生物化合物的作用模式。 本发明还涉及可作为药物筛选靶标开发的核酸序列。

公开(公告)号：US06320103B1

公开(公告)日：2001-11-20

申请号：US09687298

申请日：2000-10-12

申请人： Debbie Yaver ,  Randy M. Berka ,  Michael W. Rey

发明人： Debbie Yaver ,  Randy M. Berka ,  Michael W. Rey

IPC分类号： A01H900

CPC分类号： C12N9/0006

摘要： The present invention relates to isolated polypeptides having choline oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

摘要翻译： 本发明涉及具有胆碱氧化酶活性的分离的多肽和分离的编码多肽的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US6146864A

公开(公告)日：2000-11-14

申请号：US443087

申请日：1999-11-18

申请人： Debbie Yaver ,  Randy M. Berka ,  Michael W. Rey

发明人： Debbie Yaver ,  Randy M. Berka ,  Michael W. Rey

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12R1/19 ,  C12N9/02 ,  C07H21/04 ,  C12N1/20

CPC分类号： C12N9/0006

摘要： The present invention relates to isolated polypeptides having choline oxidase activity and isolated nucleic acid sequences encoding the polypeptide from Fusarium venenatum. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

摘要翻译： 本发明涉及具有胆碱氧化酶活性的分离的多肽和分离的编码来自镰状镰刀菌的多肽的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。