公开(公告)号：US11744250B2

公开(公告)日：2023-09-05

申请号：US17559857

申请日：2021-12-22

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Christina M. Taylor ,  Monika R. VanGordon ,  Kimberly M. Wegener ,  Brian E. Weiner

IPC: A01N63/50 ,  A01P7/04 ,  C07K14/32 ,  C12N15/82 ,  C12Q1/6895 ,  G01N33/68

CPC classification number: A01N63/50 ,  A01P7/04 ,  C07K14/32 ,  C12N15/8286 ,  C12Q1/6895 ,  G01N33/68 ,  C12Q2600/13

Abstract: Pesticidal proteins exhibiting inhibitory, suppressive, and toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4064 and TIC4064 amino acid sequence variants. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4064 and TIC4064 amino acid sequence variant pesticidal proteins are also provided.

公开(公告)号：US20230250447A1

公开(公告)日：2023-08-10

申请号：US17934287

申请日：2022-09-22

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe

IPC: C12N15/82 ,  C07K14/325 ,  G01N33/569 ,  G01N33/00 ,  A01N63/10 ,  A01N63/50

CPC classification number: C12N15/8286 ,  C07K14/325 ,  G01N33/56961 ,  G01N33/0098 ,  A01N63/10 ,  A01N63/50

Abstract: A pesticidal protein class of PirA, PirB, and PirAB fusion proteins exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the PirA, PirB, and PirAB fusion proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran, Lepidopteran, and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the PirA, PirB, and PirAB fusion proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the PirA, PirB, and PirAB fusion proteins are also provided.

公开(公告)号：US11673922B2

公开(公告)日：2023-06-13

申请号：US17561332

申请日：2021-12-23

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Todd A. Ciche ,  Arlene R. Howe ,  Stephanie C. Waldheuser ,  Kimberly M. Wegener

IPC: C07K14/32 ,  C12N15/82 ,  A01P7/04 ,  A01N63/50 ,  C07K14/325

CPC classification number: C07K14/325 ,  A01N63/50 ,  A01P7/04 ,  C12N15/8286

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.

公开(公告)号：US20220235369A1

公开(公告)日：2022-07-28

申请号：US17575318

申请日：2022-01-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flainski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  C07K14/195 ,  C07K14/32 ,  A01N63/50

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US11266151B2

公开(公告)日：2022-03-08

申请号：US16748392

申请日：2020-01-21

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Danqi Chen ,  Todd A. Ciche ,  Arlene R. Howe ,  Jennifer L. Lutke ,  Barbara E. Wiggins ,  Yuanji Zhang

IPC: A01N63/25 ,  C12N15/70 ,  C12Q1/6895 ,  C07K14/195 ,  C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3 pesticidal proteins are also provided. Also disclosed are methods and compositions to improve the insecticidal activity of a pesticidal protein against an insect pest species. Further disclosed are method and compositions to reduce expression of a pesticidal protein in the reproductive tissues of a transgenic plant.

公开(公告)号：US11254950B2

公开(公告)日：2022-02-22

申请号：US16805362

申请日：2020-02-28

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jennifer L. Lutke ,  Eric Van Fleet

IPC: C12N15/82 ,  C07K14/32 ,  A01N63/23 ,  A01N37/46

Abstract: Nucleotide sequences are disclosed encoding novel, insecticidal TIC4747 and related proteins exhibiting Hemipteran and Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC4747-related protein toxin class.

公开(公告)号：US11242540B2

公开(公告)日：2022-02-08

申请号：US15931303

申请日：2020-05-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine Chay ,  Artem Evdokimov ,  Stanislaw Flasinski ,  Uma R. Kesanapalli ,  Megan N. Schroder ,  Rachael N. Slightom ,  Nengbing Tao ,  Andrew M. Wollacott

IPC: C12N15/82 ,  A01N65/04 ,  C07K14/32

Abstract: The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

公开(公告)号：US11021715B2

公开(公告)日：2021-06-01

申请号：US16179385

申请日：2018-11-02

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Urna R. Kesanapalli ,  Jennifer L. Lutke

IPC: C12N15/82 ,  C12Q1/6895 ,  A01N37/46 ,  G01N33/53

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

公开(公告)号：US20200002718A1

公开(公告)日：2020-01-02

申请号：US16572998

申请日：2019-09-17

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine Chay ,  Artem Evdokimov ,  Stanislaw Flasinski ,  Uma R. Kesanapalli ,  Megan N. Schroder ,  Rachael N. Slightom ,  Nengbing Tao ,  Andrew M. Wollacott

IPC: C12N15/82 ,  C07K14/32

Abstract: The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

公开(公告)号：US10465205B2

公开(公告)日：2019-11-05

申请号：US15944114

申请日：2018-04-03

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  C07K14/195 ,  C07K14/32 ,  A01N37/46 ,  A01N63/02

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.