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公开(公告)号:US20200283790A1
公开(公告)日:2020-09-10
申请号:US16844949
申请日:2020-04-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor: Jintai Huang , Youlin Qi , Heping Yang , Yuanji Zhang
Abstract: The invention provides novel recombinant DNA molecules, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide molecule or recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such DNA molecules and compositions.
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公开(公告)号:US10435686B2
公开(公告)日:2019-10-08
申请号:US14567786
申请日:2014-12-11
Applicant: Monsanto Technology LLC
Inventor: Edwards M. Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalic , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
IPC: C12N15/113 , C12N15/82
Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
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公开(公告)号:US09976152B2
公开(公告)日:2018-05-22
申请号:US15065416
申请日:2016-03-09
Applicant: Monsanto Technology LLC
Inventor: Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
CPC classification number: C12N15/8218 , C12N15/8216 , C12N15/8217 , C12N15/8225 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
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公开(公告)号:US20160186194A1
公开(公告)日:2016-06-30
申请号:US15065416
申请日:2016-03-09
Applicant: Monsanto Technology LLC
Inventor: Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
IPC: C12N15/82
CPC classification number: C12N15/8218 , C12N15/8216 , C12N15/8217 , C12N15/8225 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
Abstract translation: 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地,本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因,其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞,植物和种子。
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公开(公告)号:US20160168569A1
公开(公告)日:2016-06-16
申请号:US15052062
申请日:2016-02-24
Applicant: Monsanto Technology LLC
Inventor: Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
IPC: C12N15/113 , C12N15/82
CPC classification number: C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
Abstract translation: 本发明公开了编码可用于调节一种或多种感兴趣的基因表达的分阶段的小RNA的重组DNA构建体。 本发明还公开了含有本发明的重组DNA构建体的转基因植物细胞,植物和种子。
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公开(公告)号:US09309512B2
公开(公告)日:2016-04-12
申请号:US13775477
申请日:2013-02-25
Applicant: Monsanto Technology LLC
Inventor: Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
IPC: C12N15/82 , A01H1/00 , C07H21/04 , C12N15/113
CPC classification number: C12N15/113 , C12N15/8218 , C12N15/8245 , C12N15/8254 , C12N15/8266 , C12N15/827
Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
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公开(公告)号:US11629358B2
公开(公告)日:2023-04-18
申请号:US16931427
申请日:2020-07-16
Applicant: Monsanto Technology LLC
Inventor: Clayton T. Larue , Joel E. Ream , Aabid Shariff , Yuanji Zhang , Xuefeng Zhou
IPC: C12N15/82 , C07K14/415 , C12N9/02
Abstract: The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.
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公开(公告)号:US11560574B2
公开(公告)日:2023-01-24
申请号:US16845907
申请日:2020-04-10
Applicant: Monsanto Technology LLC
Inventor: Jintai Huang , Sergey Ivashuta , Youlin Qi , Barbara Elizabeth Wiggins , Yuanji Zhang
IPC: C12N15/82
Abstract: The invention provides methods and compositions for selectively suppressing the expression of a recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides methods and compositions for inducing male sterility in a transgenic plant. Plants, plant cells, plant parts, seeds, and commodity products including such compositions are aspects of the invention.
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公开(公告)号:US10704057B2
公开(公告)日:2020-07-07
申请号:US15210725
申请日:2016-07-14
Applicant: Monsanto Technology LLC
Inventor: Jintai Huang , Youlin Qi , Heping Yang , Yuanji Zhang
Abstract: The invention provides novel recombinant DNA molecules, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide molecule or recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such DNA molecules and compositions.
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公开(公告)号:US20180044690A1
公开(公告)日:2018-02-15
申请号:US15660660
申请日:2017-07-26
Applicant: Monsanto Technology LLC
Inventor: Clayton T. Larue , Joel E. Ream , Aabid Shariff , Yuanji Zhang , Xuefeng Zhou
IPC: C12N15/82 , C07K14/415 , C12N9/02
Abstract: The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.
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