公开(公告)号：US09952153B2

公开(公告)日：2018-04-24

申请号：US13991347

申请日：2011-12-02

申请人： Torsten Schulz ,  Daniel Weicherding

发明人： Torsten Schulz ,  Daniel Weicherding

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N21/64 ,  G02C7/02 ,  B01L3/00 ,  B41M5/24 ,  B41M5/26

CPC分类号： G01N21/6402 ,  B01L3/502707 ,  B01L2300/0654 ,  B01L2300/168 ,  B41M5/24 ,  B41M5/26 ,  B41M5/267 ,  G02C7/021

摘要： A method for the transformation of material (e.g. plastic material) into an optically modulating state via laser radiation is described. The optically modulating state may be a state in which light is emitted at a different wavelength than it is absorbed. The plastic material to may be a thermoplastic or elastomeric material, or an organic polymer selected from the group consisting of polyethylene, polypropylene, polystyrene, polycarbonate and polycycloolefin. The laser radiation may comprise the application of an amount of energy of about 0.1 nJoule/μm2 to about 100 μJoule/μm2 and/or may comprise a radiation of a wavelength of about 355 nm to about 1064 nm. The optically modulating state of the plastic material may absorb light in a wavelength spectrum of about 380 nm to about 540 nm and/or a wavelength spectrum of about 635 nm to about 655 nm. The optically modulating state of the plastic material may emit light in a wavelength spectrum of about 550 nm to about 800 nm. The transformation of the plastic material may comprise the generation of optically modulating elements on the surface of said plastic material, selected from the group comprising geometrical forms, geometrical pattern, spots, dots, lines, circles, squares, characters, symbols, drawings, barcode and datamatrixcode. The material may be used as component for the manufacture of a device, microfluidic device, system, cartridge or instrument. Based on the employment of the material the usability of a device or system and/or of any procedure, function or method carried out with it or in it may be determined and/or controlled.

公开(公告)号：US20150198604A1

公开(公告)日：2015-07-16

申请号：US14564067

申请日：2014-12-08

申请人： Eugen Ermantraut ,  Thomas Kaiser ,  Jens Tuchscheerer ,  Vico Baier ,  Torsten Schulz ,  Anke Wostemeyer

发明人： Eugen Ermantraut ,  Thomas Kaiser ,  Jens Tuchscheerer ,  Vico Baier ,  Torsten Schulz ,  Anke Wostemeyer

IPC分类号： G01N33/58

CPC分类号： G01N33/58 ,  B01L3/50273 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  Y10T436/25

摘要： A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte. Capillary structures of the devices or used in the methods may comprise a matrix and the devices may comprise control elements and methods for assaying of sample may use corresponding controlling activities.

摘要翻译： 描述了用于分析多种分析物中的每一种的样品的方法。 该方法包括使间隔开的测试区域的阵列与液体样品（例如全血）接触。 测试区域设置在微流体装置的通道内。 通道由至少一个柔性壁和可以是柔性的第二壁限定。 每个测试区域包含对各个目标分析物特异的探针化合物。 微流体装置被压缩以减小通道的厚度，其是通道内的壁的内表面之间的距离。 每个分析物的存在通过光学检测在相应位置处的内表面之间的距离减小的多个测试区域中的每一个处的相互作用来确定。 每个测试区域的相互作用表明样品中目标分析物的存在。 装置的毛细结构或方法中使用的毛细管结构可以包括基质，并且装置可以包括控制元件和用于测定样品的方法可以使用相应的控制活动。

公开(公告)号：US20150177231A9

公开(公告)日：2015-06-25

申请号：US13645002

申请日：2012-10-04

申请人： Thomas Kaiser ,  Klaus-Peter Möbius ,  Torsten Schulz ,  Thomas Uhlig ,  Alexander Von Schenk Zu Schweinsberg ,  Eugen Ermantraut ,  Jens Tuchscheerer

发明人： Thomas Kaiser ,  Klaus-Peter Möbius ,  Torsten Schulz ,  Thomas Uhlig ,  Alexander Von Schenk Zu Schweinsberg ,  Eugen Ermantraut ,  Jens Tuchscheerer

IPC分类号： G01N33/53 ,  G01N21/75

摘要： A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte.

摘要翻译： 描述了用于分析多种分析物中的每一种的样品的方法。 该方法包括使间隔开的测试区域的阵列与液体样品（例如全血）接触。 测试区域设置在微流体装置的通道内。 通道由至少一个柔性壁和可以是柔性的第二壁限定。 每个测试区域包含对各个目标分析物特异的探针化合物。 微流体装置被压缩以减小通道的厚度，其是通道内的壁的内表面之间的距离。 每个分析物的存在通过光学检测在相应位置处的内表面之间的距离减小的多个测试区域中的每一个处的相互作用来确定。 每个测试区域的相互作用表明样品中目标分析物的存在。

公开(公告)号：US08969043B2

公开(公告)日：2015-03-03

申请号：US13321877

申请日：2010-05-27

申请人： Jochen Schaub ,  Torsten Schulz

发明人： Jochen Schaub ,  Torsten Schulz

IPC分类号： C12P21/00 ,  G01N33/50 ,  G01N33/92

CPC分类号： G01N33/5005 ,  G01N33/92

摘要： Biopharmaceutical process development with recombinant protein producing mammalian cells has realized a tremendous increase in both productivity and product yields in the past years. These achievements can be mainly attributed to the advancements in cell line development, media, and process optimization. Only recently, genome-scale technologies enable a system-level analysis to elucidate the complex biomolecular basis of protein production in mammalian cells promising an increased process understanding and the deduction of knowledge-based approaches for further process optimization. The present invention describes a method for a rational cell culturing process using such a knowledge-based approach.

摘要翻译： 使用重组蛋白质生产哺乳动物细胞的生物制药工艺开发在过去几年中实现了生产力和产品产量的大幅度增长。 这些成果主要归功于细胞培养，培养和工艺优化的进步。 只有最近，基因组技术才能实现系统级分析，以阐明哺乳动物细胞中蛋白质生产的复杂生物分子基础，从而有助于进一步增强过程的理解和推导基于知识的方法进一步优化工艺。 本发明描述了使用这种基于知识的方法进行合理细胞培养过程的方法。

公开(公告)号：US20140099731A1

公开(公告)日：2014-04-10

申请号：US13645002

申请日：2012-10-04

申请人： Thomas Kaiser ,  Klaus-Peter Möbius ,  Torsten Schulz ,  Thomas Uhlig ,  Alexander Von Schenk Zu Schweinsberg ,  Eugen Ermantraut ,  Jens Tuchscheerer

发明人： Thomas Kaiser ,  Klaus-Peter Möbius ,  Torsten Schulz ,  Thomas Uhlig ,  Alexander Von Schenk Zu Schweinsberg ,  Eugen Ermantraut ,  Jens Tuchscheerer

IPC分类号： G01N21/64 ,  G01N21/75

摘要： A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte.

摘要翻译： 描述了用于分析多种分析物中的每一种的样品的方法。 该方法包括使间隔开的测试区域的阵列与液体样品（例如全血）接触。 测试区域设置在微流体装置的通道内。 通道由至少一个柔性壁和可以是柔性的第二壁限定。 每个测试区域包含对各个目标分析物特异的探针化合物。 微流体装置被压缩以减小通道的厚度，其是通道内的壁的内表面之间的距离。 每个分析物的存在通过光学检测在相应位置处的内表面之间的距离减小的多个测试区域中的每一个处的相互作用来确定。 每个测试区域的相互作用表明样品中目标分析物的存在。

公开(公告)号：US20120321518A1

公开(公告)日：2012-12-20

申请号：US13597012

申请日：2012-08-28

申请人： Eugen ERMANTRAUT ,  Torsten Schulz ,  Thomas Ellinger ,  Daniel Weicherding

发明人： Eugen ERMANTRAUT ,  Torsten Schulz ,  Thomas Ellinger ,  Daniel Weicherding

IPC分类号： G01N33/53

CPC分类号： B01L3/502707 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0877 ,  B01L2400/0481 ,  C12Q1/6834

摘要： The present invention relates to devices and methods for performing assays, especially for determining the presence and/or amount of one or more analytes. In particular, the invention relates to a device for the detection of analytes, comprising a reversibly compressible matrix located between a first surface and a second surface, wherein the second surface is located opposite to the first surface, and wherein the distance between the first surface and the second surface is variable. The invention also relates to a corresponding method using such a device for the detection of one or more species of analytes.

摘要翻译： 本发明涉及用于进行测定的装置和方法，特别是用于确定一种或多种分析物的存在和/或量。 特别地，本发明涉及一种用于检测分析物的装置，包括位于第一表面和第二表面之间的可逆压缩基质，其中第二表面位于与第一表面相对的位置，并且其中第一表面 并且第二表面是可变的。 本发明还涉及使用这种装置用于检测一种或多种分析物的相应方法。

公开(公告)号：US08278113B2

公开(公告)日：2012-10-02

申请号：US12385805

申请日：2009-07-14

申请人： Eugen Ermantraut ,  Torsten Schulz ,  Thomas Ellinger ,  Daniel Weicherding

发明人： Eugen Ermantraut ,  Torsten Schulz ,  Thomas Ellinger ,  Daniel Weicherding

IPC分类号： G01N33/48

CPC分类号： B01L3/502707 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0877 ,  B01L2400/0481 ,  C12Q1/6834

摘要： The present invention relates to devices and methods for performing assays, especially for determining the presence and/or amount of one or more analytes. In particular, the invention relates to a device for the detection of analytes, comprising a reversibly compressible matrix located between a first surface and a second surface, wherein the second surface is located opposite to the first surface, and wherein the distance between the first surface and the second surface is variable. The invention also relates to a corresponding method using such a device for the detection of one or more species of analytes.

摘要翻译： 本发明涉及用于进行测定的装置和方法，特别是用于确定一种或多种分析物的存在和/或量。 特别地，本发明涉及一种用于检测分析物的装置，包括位于第一表面和第二表面之间的可逆压缩基质，其中第二表面位于与第一表面相对的位置，并且其中第一表面 并且第二表面是可变的。 本发明还涉及使用这种装置用于检测一种或多种分析物的相应方法。

公开(公告)号：US08040494B2

公开(公告)日：2011-10-18

申请号：US12092422

申请日：2006-11-06

申请人： Eugen Ermantraut ,  Ralf Bickel ,  Torsten Schulz ,  Thomas Ullrich ,  Jens Tuchscheerer

发明人： Eugen Ermantraut ,  Ralf Bickel ,  Torsten Schulz ,  Thomas Ullrich ,  Jens Tuchscheerer

IPC分类号： G06K9/74

CPC分类号： G01N33/56966 ,  B01F11/0045 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0825 ,  B01L2400/0481 ,  G01N21/03 ,  G01N35/0099 ,  G01N2021/0346 ,  G01N2035/00346

摘要： The present invention relates to devices and methods for the qualitative and/or quantitative detection of particles. In particular, the invention relates to devices for the detection of particles, comprising a reaction chamber formed within a chamber body between a first surface and a second surface, wherein the second surface is located opposite to the first surface, and one or more displacers, wherein the distance between the first surface and the second surface is variable via the one or more displacers at least in one or more parts of the surface area of the first surface and/or the second surface. The invention also relates to corresponding methods for the detection of particles, comprising positioning a sample supposed to comprise one or more species of particles to be detected in a reaction chamber, displacing at least a part of the sample within the reaction chamber via the one or more displacers; and detecting/determining a value indicative for the presence and/or number of one or more species of particles.

摘要翻译： 本发明涉及用于定性和/或定量检测颗粒的装置和方法。 特别地，本发明涉及用于检测颗粒的装置，其包括形成在第一表面和第二表面之间的室主体内的反应室，其中第二表面位于与第一表面相对的位置，以及一个或多个位移器， 其中所述第一表面和所述第二表面之间的距离可以经由所述一个或多个位移器在所述第一表面和/或所述第二表面的表面区域的至少一个或多个部分中是可变的。 本发明还涉及用于检测颗粒的相应方法，包括将假定包含待检测的一种或多种待检测颗粒的样品定位在反应室中，通过一个或多个反应室置换反应室内的至少一部分样品 更多的置换器 以及检测/确定指示一种或多种粒子的存在和/或数量的值。

公开(公告)号：USD641477S1

公开(公告)日：2011-07-12

申请号：US29380119

申请日：2010-11-30

申请人： Horst Boessneck ,  Eugen Ermantraut ,  Thomas Kaiser ,  Torsten Schulz

设计人： Horst Boessneck ,  Eugen Ermantraut ,  Thomas Kaiser ,  Torsten Schulz

公开(公告)号：US20110124114A1

公开(公告)日：2011-05-26

申请号：US12922383

申请日：2009-03-16

申请人： Eugen Ermantraut ,  Thomas Kaiser ,  Jens Tuchscheerer ,  Vico Beier ,  Torsten Schulz ,  Anke Wöstemeyer

发明人： Eugen Ermantraut ,  Thomas Kaiser ,  Jens Tuchscheerer ,  Vico Beier ,  Torsten Schulz ,  Anke Wöstemeyer

IPC分类号： G01N21/00 ,  G01N33/00 ,  G01N1/00

CPC分类号： G01N33/58 ,  B01L3/50273 ,  B01L2200/025 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  Y10T436/25

摘要： A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte. Capillary structures of the devices or used in the methods may comprise a matrix and the devices may comprise control elements and methods for assaying of sample may use corresponding controlling activities.

摘要翻译： 描述了用于分析多种分析物中的每一种的样品的方法。 该方法包括使间隔开的测试区域的阵列与液体样品（例如全血）接触。 测试区域设置在微流体装置的通道内。 通道由至少一个柔性壁和可以是柔性的第二壁限定。 每个测试区域包含对各个目标分析物特异的探针化合物。 微流体装置被压缩以减小通道的厚度，其是通道内的壁的内表面之间的距离。 每个分析物的存在通过光学检测在相应位置处的内表面之间的距离减小的多个测试区域中的每一个处的相互作用来确定。 每个测试区域的相互作用表明样品中目标分析物的存在。 装置的毛细结构或方法中使用的毛细管结构可以包括基质，并且装置可以包括控制元件和用于测定样品的方法可以使用相应的控制活动。