METHOD OF CHARACTERIZING ENDOGENOUS POLYNUCLEOTIDE-POLYPEPTIDE INTERACTIONS
    76.
    发明申请
    METHOD OF CHARACTERIZING ENDOGENOUS POLYNUCLEOTIDE-POLYPEPTIDE INTERACTIONS 审中-公开
    表征内源多核苷酸多肽交互的方法

    公开(公告)号:US20090305272A1

    公开(公告)日:2009-12-10

    申请号:US12348630

    申请日:2009-01-05

    IPC分类号: C12Q1/68 G01N33/53 C12N15/63

    CPC分类号: G01N33/5308 G01N33/536

    摘要: A method for characterizing an endogenous polypeptide includes introducing epitope tag-encoding polynucleotide into an endogenous locus of a somatic cell by homogenous recombination mediated knock-in so that an epitope tagged endogenous polypeptide is expressed by the cell, and characterizing the epitope tagged endogenous polypeptide using an immunoassay.

    摘要翻译: 用于表征内源性多肽的方法包括通过均质重组介导的敲入将表达标签编码多核苷酸引入体细胞的内源基因座,使得表位标记的内源多肽由细胞表达,并使用 免疫测定

    DC path checking in a hierarchical circuit design
    77.
    发明授权
    DC path checking in a hierarchical circuit design 失效
    DC路径检查在分层电路设计中

    公开(公告)号:US07412681B1

    公开(公告)日:2008-08-12

    申请号:US11067571

    申请日:2005-02-25

    IPC分类号: G06F17/50

    CPC分类号: G06F17/5036

    摘要: A computer implemented method is provided for use in evaluating a hierarchical representation of a circuit design encoded in a computer readable medium comprising: traversing a circuit path within a higher level circuit that includes a reference potential connection, to identify a port of a call to a first lower level circuit that is DC path connected to the reference potential; identifying a first DC port group that includes each port of the call to the first lower level circuit that is DC path connected to the identified port of the call to the first lower level circuit; automatically marking as DC path connected to the reference potential, each port of the call to the first lower level circuit that is a member of the first DC port group; and traversing a circuit path within the first lower level circuit to identify a circuit path within the first lower level circuit that is DC path connected to a marked port of the first lower level circuit.

    摘要翻译: 提供了一种用于评估在计算机可读介质中编码的电路设计的分层表示的计算机实现的方法,包括:遍历包括参考电位连接的更高级电路内的电路路径,以识别对 第一低电平电路,其是连接到参考电位的DC路径; 识别包括与所述第一下层电路的呼叫的每个端口的第一DC端口组,所述第一下层电路是连接到所识别的对所述第一下级电路的呼叫端口的DC路径; 自动标记为连接到参考电位的DC路径,调用作为第一DC端口组成员的第一下层电路的每个端口; 并且穿过第一下层电路内的电路,以识别第一下层电路内的电路路径,该路径是连接到第一下层电路的标记端口的DC路径。

    System and method for streaming media objects
    78.
    发明申请
    System and method for streaming media objects 有权
    用于流媒体对象的系统和方法

    公开(公告)号:US20070266169A1

    公开(公告)日:2007-11-15

    申请号:US11431681

    申请日:2006-05-10

    IPC分类号: G06F15/16

    摘要: A method and system for streaming media objects. A streaming system includes a group of end nodes and at least one core node connected via an overlay network. The end nodes cache segments of media objects and stream the media objects to a client segment by segment. Each end node stores segment indices for locating segments cached on end nodes or a core node in the system. The core node is a dedicated proxy which fetches media objects from a remote media server and streams the media objects to a client when the media objects are unavailable from the end nodes.

    摘要翻译: 一种用于流媒体对象的方法和系统。 流系统包括一组终端节点和经由覆盖网络连接的至少一个核心节点。 终端节点缓存媒体对象的分段,并将媒体对象逐段流式传输到客户端。 每个终端节点存储用于定位缓存在终端节点上的段或系统中的核心节点的段索引。 核心节点是从远程媒体服务器获取媒体对象的专用代理,并且当媒体对象从终端节点不可用时,将媒体对象传送到客户端。