利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

241 条记录 (耗时 0.043 秒)

    SODIUM CHLORIDE SOLUTION FOR DRUG RECONSTITUTION OR DILUTION
    82.
    发明申请
    SODIUM CHLORIDE SOLUTION FOR DRUG RECONSTITUTION OR DILUTION 审中-公开

    公开(公告)号：US20200338199A1

    公开(公告)日：2020-10-29

    申请号：US16946998

    申请日：2020-07-14

    申请人： Wyeth LLC

    发明人： Chandra A Webb ,  Julie Zerfas

    IPC分类号： A61K47/02 ,  A61K9/00 ,  A61K38/48 ,  A61K9/19 ,  A61K9/08

    摘要： The invention provides methods for preparing pharmaceutical formulations for injection such that upon injection the formulation does not cause erythrocyte agglutination, hemolysis, and/or cell shrinkage. To prevent agglutination, a pharmaceutical formulation ready for injection needs to have a sufficient ionic strength. To prevent hemolysis or cell shrinkage, a pharmaceutical formulation ready for injection needs to be about isotonic with respect to plasma. The invention provides methods that prepare pharmaceutical formulations for injection that have both the sufficient ionic strength to prevent agglutination and the requisite tonicity to prevent significant hemolysis or cell dehydration or shrinkage. The present methods involve the use of sodium chloride solutions that are about 25 mM to about 150 mM for reconstituting lyophilized cakes (or other non-liquid pharmaceutical formulations) into solution or for diluting pharmaceutical formulation solutions.

    Use Of Perfusion To Enhance Production Of Fed-batch Cell Culture In Bioreactors
    83.
    发明申请
    Use Of Perfusion To Enhance Production Of Fed-batch Cell Culture In Bioreactors 审中-公开

    公开(公告)号：US20200325441A1

    公开(公告)日：2020-10-15

    申请号：US16907799

    申请日：2020-06-22

    申请人： Wyeth LLC

    发明人： Gregory Walter HILLER

    IPC分类号： C12N5/00 ,  C12M1/00 ,  C12P21/00

    摘要： The invention relates to methods of improving protein production, e.g., large-scale commercial protein production, e.g., antibody production, utilizing a modified fed-batch cell culture method comprising a cell growth phase and a polypeptide production phase. The modified fed-batch cell culture method combines both cell culture perfusion and fed-batch methods to achieve higher titers of polypeptide products. Because the modified fed-batch cell culture method of the invention produces higher polypeptide product titers than fed-batch culture alone, it will substantially improve commercial-scale protein production. The invention also relates to a perfusion bioreactor apparatus comprising a fresh medium reservoir connected to a bioreactor by a feed pump, a recirculation loop connected to the bioreactor, wherein the recirculation loop comprises a filtration device, e.g., ultrafiltration or microfiltration, and a permeate pump connecting the filtration device to a permeate collection container.