公开(公告)号：US08237016B2

公开(公告)日：2012-08-07

申请号：US13271666

申请日：2011-10-12

申请人： Xudong Ye ,  Michael W. Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul S. Chomet

发明人： Xudong Ye ,  Michael W. Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul S. Chomet

IPC分类号： C12N15/82 ,  C12N15/31 ,  C12N15/52 ,  C12N5/10 ,  C12N1/21 ,  A01H5/00

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C12N9/1085 ,  C12N15/8205 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

摘要翻译： 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。

公开(公告)号：US20110258734A1

公开(公告)日：2011-10-20

申请号：US13046601

申请日：2011-03-11

申请人： Thomas Adams ,  Ameeta K. Agarwa ,  Jeffrey Ahrens ,  James A. Ball ,  Amarjit Basra ,  Erin Bell ,  Terry L. Bradshaw ,  Paul S. Chomet ,  James H. Crowley ,  Jill Deikman ,  Molian Deng ,  Meghan Donnarummo ,  Kimberly Faye Zobrist Duff ,  Stephen Duff ,  Michael D. Edgerton ,  Banu Gopalan ,  Xiaoping He ,  Mackenzie Heal ,  Brendan S. Hinchey ,  Shihshieh Huang ,  Richard G. Johnson ,  Vincent Jung ,  Keith Kretzmer ,  Lucille B. Laccetti ,  Chao Qiang Lai ,  Garrett J. Lee ,  Michael H. Luethy ,  Timothy J. Leland ,  Jingdong Liu ,  Bin Lu ,  Adrian A. Lund ,  Linda L. Madson ,  Kathleen P. Malloy ,  Marcus Paul McNabnay ,  Manchikanti Padmavati ,  Thomas Ruff ,  William Start ,  Daniel Tennessen ,  Carl P. Urwin ,  Gabriela Vaduva ,  K.R. Vidya ,  Steven Thomas Voss ,  Christine Voyles ,  Zhango Xin ,  Nanfei Xu ,  Qingzhang Xu ,  Qiang Zhang ,  Yajuan Zhao ,  Li Zhou

发明人： Thomas Adams ,  Ameeta K. Agarwa ,  Jeffrey Ahrens ,  James A. Ball ,  Amarjit Basra ,  Erin Bell ,  Terry L. Bradshaw ,  Paul S. Chomet ,  James H. Crowley ,  Jill Deikman ,  Molian Deng ,  Meghan Donnarummo ,  Kimberly Faye Zobrist Duff ,  Stephen Duff ,  Michael D. Edgerton ,  Banu Gopalan ,  Xiaoping He ,  Mackenzie Heal ,  Brendan S. Hinchey ,  Shihshieh Huang ,  Richard G. Johnson ,  Vincent Jung ,  Keith Kretzmer ,  Lucille B. Laccetti ,  Chao Qiang Lai ,  Garrett J. Lee ,  Michael H. Luethy ,  Timothy J. Leland ,  Jingdong Liu ,  Bin Lu ,  Adrian A. Lund ,  Linda L. Madson ,  Kathleen P. Malloy ,  Marcus Paul McNabnay ,  Manchikanti Padmavati ,  Thomas Ruff ,  William Start ,  Daniel Tennessen ,  Carl P. Urwin ,  Gabriela Vaduva ,  K.R. Vidya ,  Steven Thomas Voss ,  Christine Voyles ,  Zhango Xin ,  Nanfei Xu ,  Qingzhang Xu ,  Qiang Zhang ,  Yajuan Zhao ,  Li Zhou

IPC分类号： A01H1/02 ,  A01D45/00 ,  A01H5/10 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8279 ,  A01D45/00 ,  A01N25/34 ,  A01N31/16 ,  A01N41/10 ,  A01N43/40 ,  A01N43/653 ,  A01N43/78 ,  A01N43/80 ,  A01N55/02 ,  C07K14/415 ,  C12N9/0071 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  C12Y114/12017 ,  A01N25/10 ,  A01N25/02 ,  A01N2300/00

摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20080064866A1

公开(公告)日：2008-03-13

申请号：US11856978

申请日：2007-09-18

申请人： Toni Armstrong ,  Kenneth Barton ,  Laura Crow ,  Larry Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian Martinell ,  Michael Petersen ,  Kenneth Piller ,  Qi Wang ,  Xudong Ye

发明人： Toni Armstrong ,  Kenneth Barton ,  Laura Crow ,  Larry Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian Martinell ,  Michael Petersen ,  Kenneth Piller ,  Qi Wang ,  Xudong Ye

IPC分类号： C07H21/04

CPC分类号： C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

公开(公告)号：US20070266456A1

公开(公告)日：2007-11-15

申请号：US11747824

申请日：2007-05-11

申请人： Xudong Ye ,  Michael Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul Chomet

发明人： Xudong Ye ,  Michael Petersen ,  Larry Gilbertson ,  David Walters ,  Susan Johnson ,  Shihshieh Huang ,  Paul Chomet

IPC分类号： A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/20 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C12N9/1085 ,  C12N15/8205 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8218 ,  C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

摘要翻译： 本发明提供用于鉴定含有感兴趣的转基因但缺少标记基因的转基因种子的方法和组合物。 使用导致可检测表型的鉴定序列增加筛选未与目的基因连接的转基因序列与编码感兴趣的基因的序列分离的种子和植物的效率。