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    Sucrose phosphate synthase nucleic acid molecules and uses therefor
    5.
    发明申请
    Sucrose phosphate synthase nucleic acid molecules and uses therefor 审中-公开
    蔗糖磷酸合成酶核酸分子及其用途

    公开(公告)号：US20050251882A1

    公开(公告)日：2005-11-10

    申请号：US10336263

    申请日：2003-01-03

    Applicant: Robert D'Ordine ,  Stanton Dotson ,  Pamela Sisson ,  Stephen Duff

    Inventor： Robert D'Ordine ,  Stanton Dotson ,  Pamela Sisson ,  Stephen Duff

    IPC: A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/10 ,  C12N15/82

    CPC classification number: C12N15/8245 ,  C12N9/1066

    Abstract: The present invention relates generally to plant molecular biology and genetic engineering. In one embodiment, the present invention relates to isolated nucleic acids from cyanobacteria encoding sucrose phosphate synthase (SPS) or SPS-like proteins, in another embodiment, the present invention relates to isolated nucleic acids from maize plants encoding sucrose phosphate synthase (SPS) proteins. Each protein disclosed has utility in improving agronomic, horticultural and/or quality traits of plants, including yield.

    Abstract translation: 本发明一般涉及植物分子生物学和遗传工程。 在一个实施方案中，本发明涉及来自编码蔗糖磷酸合成酶（SPS）或SPS样蛋白质的蓝细菌的分离的核酸，在另一个实施方案中，本发明涉及编码蔗糖磷酸合成酶（SPS）蛋白质的玉米植物的分离的核酸 。 所公开的每种蛋白质都可用于改善植物的农艺，园艺和/或品质性状，包括产量。

    Nonsymbiotic plant hemoglobins to maintain cell energy status
    7.
    发明授权
    Nonsymbiotic plant hemoglobins to maintain cell energy status 失效
    非共生植物血红蛋白维持细胞能量状态

    公开(公告)号：US06936749B1

    公开(公告)日：2005-08-30

    申请号：US09720206

    申请日：1999-06-24

    Applicant: Phillip Guy ,  Stephen Duff ,  Nie Xianzhou ,  Robert Hill ,  Douglas Durnin ,  Aleksander Sowa

    Inventor： Phillip Guy ,  Stephen Duff ,  Nie Xianzhou ,  Robert Hill ,  Douglas Durnin ,  Aleksander Sowa

    IPC: A01H5/00 ,  A61K35/12 ,  C07K14/415 ,  C07K14/805 ,  C12N1/20 ,  C12N15/29 ,  C12N15/82

    CPC classification number: C07K14/415 ,  A61K35/12 ,  C07K14/805 ,  C12N1/20 ,  C12N15/8261 ,  Y02A40/146

    Abstract: Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB+) or antisense (HB−) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB− cells, where ATP levels declined by 27% and 61% respectively. Energy charge was relatively unaffected by the treatment in HB+ and wild type cells, but was reduced from 0.91 to 0.73 in HB− cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.

    Abstract translation: 非共生血红蛋白在进化中广泛存在; 然而，这些蛋白质的功能是未知的。 培养的玉米细胞已被转化以组合式表达大鼠血红蛋白基因（HB + />）或反义（HB ）方向。 在正常大气条件下，转化细胞系中的血红蛋白蛋白质相对较高或低于野生型细胞。 通过将细胞置于氮气气氛中12小时来限制氧气供应对组成型表达血红蛋白的细胞的能量状态几乎没有影响，但对野生型和HB 细胞都具有显着的作用， ATP水平分别下降了27％和61％。 能量电荷相对不受HB +和/或野生型细胞治疗的影响，但在HB 细胞中从0.91降低至0.73，表明后者不能维持 低氧状态下的能量状态。 用Hb表达载体转化的铜绿假单胞菌细胞观察到类似的结果。 建议非共生血红蛋白起作用以维持低氧环境中细胞的能量状态，并通过促进糖酵解通过NADH氧化来实现这一作用，从而增加底物水平的磷酸化。 非共生血红蛋白可能是早期形式的血红蛋白的祖先，其在低氧环境中螯合氧，提供氧气来氧化NADH以提供ATP用于细胞生长和发育。 这反过来表明，含有较高水平的Hb蛋白的细胞将在低氧压力或高能量需求下生存更长时间。

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