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119 条记录 (耗时 0.074 秒)

    Methods for increasing product yields
    81.
    发明授权
    Methods for increasing product yields 有权
    提高产品产量的方法

    公开(公告)号：US08445244B2

    公开(公告)日：2013-05-21

    申请号：US13011788

    申请日：2011-01-21

    申请人： Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC分类号： C12N9/00 ,  C12N1/20

    CPC分类号： C12P7/42 ,  C12N9/0008 ,  C12N9/0095 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  C12P7/52 ,  Y02E50/10

    摘要： A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    摘要翻译： 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间段内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为结构单元的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

    Method for redesign of microbial production systems
    82.
    发明授权
    Method for redesign of microbial production systems 有权
    微生物生产系统重新设计方法

    公开(公告)号：US08108152B2

    公开(公告)日：2012-01-31

    申请号：US12886649

    申请日：2010-09-21

    申请人： Costas D. Maranas ,  Anthony P. Burgard ,  Priti Pharkya

    发明人： Costas D. Maranas ,  Anthony P. Burgard ,  Priti Pharkya

    IPC分类号： G06F19/12 ,  G06F19/14

    CPC分类号： G01N33/5005 ,  G01N33/54366 ,  G06F19/12 ,  G06F19/18

    摘要： A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.

    摘要翻译： 用于鉴定添加到生物体特异性代谢网络以实现宿主期望生物转化的功能的计算机辅助方法包括从通用数据库获得反应以提供化学计量平衡，至少部分地基于化学计量平衡路径， 反应和底物以使生产宿主中的非天然功能数量最小化，并将至少一种化学计量平衡的途径并入宿主以提供所需的生物转化。 可以存储经修改的代谢网络的表示。

    Methods for increasing product yields
    84.
    发明授权
    Methods for increasing product yields 有权

    公开(公告)号：US08236539B2

    公开(公告)日：2012-08-07

    申请号：US13011788

    申请日：2011-01-21

    申请人： Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    发明人： Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC分类号： C12N9/00 ,  C12N1/20

    摘要： A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS
    88.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS 有权
    用于生产对苯二酸和其他化合物的微生物

    公开(公告)号：US20100330626A1

    公开(公告)日：2010-12-30

    申请号：US12875084

    申请日：2010-09-02

    申请人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    发明人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    IPC分类号： C12P17/10 ,  C12N1/00 ,  C12P7/46

    CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

    摘要： The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

    摘要翻译： 本发明提供了具有己二酸，6-氨基己酸或己内酰胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应己二酸，6-氨基己酸或己内酰胺途径中的酶的外源核酸。 本发明另外提供了生产己二酸，6-氨基己酸或己内酰胺的方法。 该方法可以包括培养己二酸，6-氨基己酸或己内酰胺生产微生物，其中微生物生物体表达至少一种编码己二酸，6-氨基己酸或己内酰胺途径酶的外源核酸，其量足以产生相应的产物 在条件下和足够的时间内生产己二酸，6-氨基己酸或己内酰胺。