PROCESS FOR SEEDING A SOLID LIGNOCELLULOSIC MATERIAL WITH A FUNGAL BIOMASS

    公开(公告)号:US20240074363A1

    公开(公告)日:2024-03-07

    申请号:US18282514

    申请日:2022-03-15

    IPC分类号: A01G18/20 C08L1/02 C08L97/02

    CPC分类号: A01G18/20 C08L1/02 C08L97/02

    摘要: The invention relates to a process for preparing a solid lignocellulosic material (1), referred to as a composite material, seeded with at least one organism (2), referred to as a filamentous fungus, which is a mycelium-forming multicellular eukaryote, in which:



    at least one solid lignocellulosic material (3) impregnated with an aqueous composition (5) is subjected to a treatment (6), known as a thermomechanical treatment, in which

    said at least one impregnated lignocellulosic material (3) is subjected to a succession of mechanical compression, expansion and shearing phases by blending at least one solid lignocellulosic material (4) of said at least one impregnated lignocellulosic material (3), in contact with the aqueous composition (5);
    said at least one impregnated lignocellulosic material (3) is brought to a temperature above 50° C.;

    whereby a composition (7), referred to as a hydrated composition, comprising a solid lignocellulosic material (8), referred to as hydrated lignocellulosic material, the specific surface area and moisture content of which are increased relative to the specific surface area and moisture content of said at least one starting lignocellulosic material (4), is formed, said hydrated lignocellulosic material (8) being suitable for being colonized by said at least one filamentous fungus (2); and then



    a composition, known as a fungal composition (9), comprising said filamentous fungus (2) is added to said hydrated composition (7) during blending;

    in which process the successive steps are performed continuously in at least one twin-screw extruder (10).

    CULTURE MEDIUM, FUNGUS BED, BAGGED FUNGUS BED, CULTURE MEDIUM MANUFACTURING METHOD, FUNGUS BED MANUFACTURING METHOD, BAGGED FUNGUS BED MANUFACTURING METHOD

    公开(公告)号:US20230159404A1

    公开(公告)日:2023-05-25

    申请号:US17916767

    申请日:2021-05-11

    申请人: LaLa Corporation

    发明人: Hiromi KOMABA

    IPC分类号: C05F11/08 C12N1/20 A01G18/20

    CPC分类号: C05F11/08 C12N1/20 A01G18/20

    摘要: A culture medium, a fungal bed, and a bagged fungal bed each of which is less liable to have mold occurring therein, and methods of producing those; wherein the culture medium contains at least one or two or more of a Bacillus sp. bacterium, an Ewingella sp. bacterium, and a Pseudomonas sp. bacterium. The fungal bed includes the culture medium inoculated with an inoculum, and the bagged fungal bed includes the fungal bed accommodated in a film bag having fine perforations. The culture medium production method incudes: heating a substrate in a storage container; and supplying air into the storage container. The fungal bed production method includes inoculating a culture medium, which is produced by the culture medium production method, with an inoculum of a mushroom, and the method of producing a bagged fungal bed includes accommodating the fungal bed in a film bag having a plurality of fine perforations.

    METHODS OF PRODUCING SOMATIC HYBRID AND CYBRID ORGANISMS

    公开(公告)号:US20230080394A1

    公开(公告)日:2023-03-16

    申请号:US17945814

    申请日:2022-09-15

    发明人: Jerred Tudela

    摘要: Methods of producing a hybrid organism. The methods include providing a first organism, providing a second organism, providing a fusing medium, combining the first organism and the second organism in or on the fusing medium to define a fusion environment, and initiating somatic fusion between the first organism and the second organism in the fusion environment to produce the hybrid organism. The first organism is either a plant organism or a fungus organism. The second organism is either a plant organism or a fungus organism. In some examples, the first organism is Tuber melanosporum . In certain examples, the second organism is either Panaeolus cambodginiensis or Panaeolus cyanescens . In some examples, the methods include incubating the hybrid organism, regenerating cell walls of the hybrid organism, and/or replicating the hybrid organism.

    CULTIVATION METHOD OF MORCHELLA WITHOUT NUTRIENT BAG

    公开(公告)号:US20230055502A1

    公开(公告)日:2023-02-23

    申请号:US17704843

    申请日:2022-03-25

    IPC分类号: A01G18/20 A01G18/40

    摘要: The present disclosure provides a cultivation method of morels and belongs to the technical field of cultivation of edible fungi. After nutrient contents that can be metabolized and utilized by morel mycelia are scientifically matched, sterilization treatment of exogenous nutrients is eliminated, and the exogenous nutrients are directly compressed for forming. Formed exogenous nutrient blocks and spawn is sowed and covered with soil, so as to realize a potential difference between “rich” and “poor” nutrients in a physical space. When the spawn germinate to form a mycelium network, the mycelia will enter a nutrient-rich exogenous nutrient area, secrete various extracellular enzymes to decompose and utilize these exogenous nutrient blocks, and transport them to a nutrient-poor mycelium network for storage, so as to complete nutrient assimilation and absorption, and reserve energy for later sexual reproduction.

    Device for growing mushrooms
    87.
    发明授权

    公开(公告)号:US11523568B2

    公开(公告)日:2022-12-13

    申请号:US17179997

    申请日:2021-02-19

    IPC分类号: A01G18/62 A01G18/69 A01G18/20

    摘要: The present invention relates to a device for growing mushrooms, comprising a shelving, arranged for supporting beds for holding compost, said beds for holding compost wherein the beds are placed at a mutual distance above each other characterized in that the beds are movable between at least a first position, wherein a second bed supported above a first bed at least partially impedes the accessibility of the first bed in a direction perpendicular to the plane in which the first bed extends and a second position in which at least a larger part of the first bed is free approachable from a direction perpendicular to the plane in which the first bed extends than in the first position.

    Growth media for improved growth and yield of fungus using treated lignocellulosic biomass

    公开(公告)号:US11427513B2

    公开(公告)日:2022-08-30

    申请号:US17048516

    申请日:2019-04-15

    申请人: LUSBIO, INC.

    发明人: Robert Bartek

    摘要: A growth medium for fungus is obtained by: (a) treating a mixture of lignocellulosic biomass and water with at least one oxidizing agent and steam at a temperature in a range of from about 130 C to about 220 C for a period from about 5 seconds to about 10 hours; (b) periodically measuring a pH of the mixture for substantially an entire duration of the treating step; and (c) as necessary, based on the pH of the mixture measured in step (b), adjusting the pH of the mixture into a range of from about pH 4.5 to about pH 7.5 by adding abase to the mixture. A method for producing the growth medium for fungi and a method for cultivating fungi are also provided.

    Monokaryon mycelial material and related method of production

    公开(公告)号:US11277981B2

    公开(公告)日:2022-03-22

    申请号:US16663269

    申请日:2019-10-24

    申请人: Mycoworks, Inc.

    发明人: Philip Ross

    摘要: A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.