公开(公告)号：US20220056093A1

公开(公告)日：2022-02-24

申请号：US17275662

申请日：2019-09-11

申请人： Ambrx, Inc.

发明人： Sigeng CHEN ,  Yingchun Lu ,  MD Harunur Rashid ,  Nickolas Knudsen ,  Feng Tian

IPC分类号： C07K14/55 ,  A61K47/60

摘要： The present invention provides methods for targeting interleukin-2 receptor-expressing cells, and, in particular, inhibiting the growth of such cells by using an interleukin-2 (IL-2) variant conjugated to a biologically active molecule that will affect cells expressing the interleukin-2 receptor.

公开(公告)号：US10800856B2

公开(公告)日：2020-10-13

申请号：US14406190

申请日：2013-06-07

申请人： Ambrx, Inc.

发明人： Richard S. Barnett ,  Feng Tian ,  Anna-Maria A. Hays Putnam ,  Marco Gymnopoulos ,  Nick Knudsen ,  Andrew Beck ,  Ying Sun

IPC分类号： A61K47/68 ,  A61K49/00 ,  C07K16/40 ,  C07K16/30 ,  C07K5/02

摘要： This invention relates to prostate-specific membrane antigen (PSMA) antibodies and antibody drug conjugates comprising at least one non-naturally-encoded amino acid. Disclosed herein are αPSMA antibodies with one or more non-naturally encoded amino acids and further disclosed are antibody drug conjugates wherein the αPSMA antibodies of the invention are conjugated to one or more toxins. Also disclosed herein are non-natural amino acid dolastatin analogs that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such dolastatin analogs. Typically, the modified dolastatin analogs include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid antibody drug conjugates, dolastatin analogs, and modified non-natural amino acid dolastatin analogs, including therapeutic, diagnostic, and other biotechnology uses.

公开(公告)号：US10751391B2

公开(公告)日：2020-08-25

申请号：US16285986

申请日：2019-02-26

申请人： AMBRX, INC.

发明人： Vadim Kraynov ,  Nick Knudsen ,  Amha Hewet ,  Kristine De Dios ,  Jason Pinkstaff ,  Lorraine Sullivan

IPC分类号： A61K38/18 ,  A61K38/22 ,  C07K14/64

摘要： Modified relaxin polypeptides and their uses thereof are provided. Exemplary embodiments provide relaxin polypeptides which include one or more amino acid substitutions with natural or non-naturally encoded amino acids, and/or linkage to a water-soluble polymer, such as polyethylene glycol. Additionally, use of said relaxin polypeptides for treatment of disease, such as heart failure, is also provided.

公开(公告)号：US09868972B2

公开(公告)日：2018-01-16

申请号：US14820318

申请日：2015-08-06

申请人： Ambrx, Inc.

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12P21/00 ,  C12P21/02 ,  C12N9/00 ,  C12N15/11

CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/11

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.

公开(公告)号：US09517273B2

公开(公告)日：2016-12-13

申请号：US14680543

申请日：2015-04-07

申请人： Ambrx, Inc.

发明人： Thomas P. Cujec ,  Roberto Mariani ,  Anna-Maria A. Hays Putnam ,  Wiliam M. Keefe ,  Nick Knudsen ,  Lillian Skidmore ,  Jason Pinkstaff ,  Vadim Kraynov

IPC分类号： A61K38/18 ,  C12P21/00 ,  C07K14/50 ,  A61K47/48 ,  A61K45/06 ,  A61K38/00

CPC分类号： C07K14/50 ,  A61K38/00 ,  A61K38/1825 ,  A61K45/06 ,  A61K47/60 ,  C12N15/70 ,  Y02A50/473

摘要： Modified FGF-21 polypeptides and uses thereof are provided.

摘要翻译： 提供了修饰的FGF-21多肽及其用途。

公开(公告)号：US09133495B2

公开(公告)日：2015-09-15

申请号：US12440015

申请日：2007-09-07

申请人： Feng Tian ,  Thea Norman ,  Stephanie Chu

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12N15/85 ,  C12P21/02 ,  C12N9/00 ,  C12N15/11

CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/11

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.

摘要翻译： 本发明提供用于生产翻译成分的组合物和方法，其扩展脊椎动物细胞中遗传编码氨基酸的数目。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸的正交对。 还提供了在脊椎动物细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。 本发明提供具有翻译成分的脊椎动物细胞，例如正交氨基-tRNA合成酶（O-RS）和正交tRNA（O-tRNA）及其各自成分的对，其用于脊椎动物蛋白质生物合成机制以掺入非天然氨基 在生长的多肽链中，在脊椎动物细胞中。

公开(公告)号：US20150080559A1

公开(公告)日：2015-03-19

申请号：US14122667

申请日：2012-05-24

申请人： AMBRX, INC.

发明人： Zhenwei Miao ,  Kyle Atkinson ,  Sandra Biroc ,  Timothy Buss ,  Melissa Neal ,  Vadim Kraynov ,  Robin Mardsen ,  Jason Pinkstaff ,  Lillian Skidmore ,  Ying Sun ,  Agnieszka Szydlik ,  Ianina Valenta

IPC分类号： C07K5/06 ,  C07K16/28 ,  A61K47/48

CPC分类号： C07K5/06 ,  A61K31/337 ,  A61K31/427 ,  A61K39/39558 ,  A61K47/6803 ,  A61K47/6855 ,  A61K2039/505 ,  C07D207/325 ,  C07D401/12 ,  C07D417/12 ,  C07K5/02 ,  C07K16/2896 ,  C07K2317/24 ,  C07K2317/76 ,  A61K2300/00

摘要： Disclosed herein are non-natural amino acids and dolastatin analogs that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The dolastatin analogs can include a wide range of possible functionalities, but typically have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are non-natural amino acid dolastatin analogs that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such dolastatin analogs. Typically, the modified dolastatin analogs include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid dolastatin analogs and modified non-natural amino acid dolastatin analogs, including therapeutic, diagnostic, and other biotechnology uses.

摘要翻译： 本文公开了包含至少一种非天然氨基酸的非天然氨基酸和多拉斯达汀类似物，以及用于制备这种非天然氨基酸和多肽的方法。 多拉司他汀类似物可以包括广泛的可能功能，但通常具有至少一个肟，羰基，二羰基和/或羟胺基团。 本文还公开了在翻译后进一步修饰的非天然氨基酸多拉司他汀类似物，用于实现这种修饰的方法，以及纯化这种多拉司他汀类似物的方法。 通常，修饰的多拉司他汀类似物包括至少一个肟，羰基，二羰基和/或羟胺基团。 进一步披露的是使用这种非天然氨基酸多拉司他汀类似物和经修饰的非天然氨基酸多拉司他丁类似物的方法，包括治疗，诊断和其他生物技术用途。

公开(公告)号：US08809511B2

公开(公告)日：2014-08-19

申请号：US13006335

申请日：2011-01-13

申请人： Zhenwei Miao ,  Junjie Liu ,  Thea Norman ,  Russell Driver

发明人： Zhenwei Miao ,  Junjie Liu ,  Thea Norman ,  Russell Driver

IPC分类号： C07K17/08 ,  C07K14/00

CPC分类号： C07C225/10 ,  A61K31/198 ,  A61K38/17 ,  A61K47/08 ,  A61K47/10 ,  A61K47/34 ,  A61K47/60 ,  C07C229/36 ,  C07C237/32 ,  C07C251/28 ,  C07C251/54 ,  C07C251/60 ,  C07C251/86 ,  C07C323/63 ,  C07C2601/08 ,  C07D209/38 ,  C07D209/46 ,  C07D307/83 ,  C07D307/88 ,  C07D307/89 ,  C07D317/22 ,  C07D317/26 ,  C07D317/28 ,  C07D317/30 ,  C07D333/72 ,  C07K14/61 ,  C12P21/02 ,  G01N33/5008 ,  G01N33/68 ,  G01N33/6848 ,  G01N2500/00 ,  Y02A50/473 ,  Y02P20/55

摘要： Disclosed herein are compounds useful in the preparation non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are compound useful in the preparation of non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses.

摘要翻译： 本文公开了可用于制备非天然氨基酸和多肽的化合物，其包括至少一种非天然氨基酸，以及制备这种非天然氨基酸和多肽的方法。 非天然氨基酸本身或作为多肽的一部分可以包括广泛的可能的官能团，但典型的具有至少一个肟，羰基，二羰基和/或羟胺基团。 本文还公开了可用于制备在翻译后进一步修饰的非天然氨基酸多肽的化合物，用于实现这些修饰的方法，以及纯化这些多肽的方法。 通常，修饰的非天然氨基酸多肽包括至少一个肟，羰基，二羰基和/或羟胺基团。 进一步披露的是使用这种非天然氨基酸多肽和修饰的非天然氨基酸多肽的方法，包括治疗，诊断和其他生物技术用途。

公开(公告)号：US08569233B2

公开(公告)日：2013-10-29

申请号：US13556754

申请日：2012-07-24

申请人： Feng Tian ,  Anna-Maria A. Hays Putnam ,  Frank Song ,  Stephanie Chu ,  Joseph Sheffer ,  Richard S. Barnett ,  Marc Siladi ,  Kyle Atkinson ,  Darin Lee ,  Peter C. Canning

发明人： Feng Tian ,  Anna-Maria A. Hays Putnam ,  Frank Song ,  Stephanie Chu ,  Joseph Sheffer ,  Richard S. Barnett ,  Marc Siladi ,  Kyle Atkinson ,  Darin Lee ,  Peter C. Canning

IPC分类号： A61K38/18 ,  A61K39/00

CPC分类号： C07K14/505 ,  A61K38/00 ,  A61K38/1816 ,  A61K47/60 ,  C12N15/102

摘要： Modified animal erythropoietin polypeptides and uses thereof are provide.

摘要翻译： 提供了改良的动物促红细胞生成素多肽及其用途。

公开(公告)号：US20130280301A1

公开(公告)日：2013-10-24

申请号：US13936201

申请日：2013-07-07

申请人： AMBRX, INC.

发明人： Feng TIAN ,  Brad HEHLI

IPC分类号： A61K39/108

CPC分类号： C12N15/70 ,  A61K39/0258 ,  C12N1/36 ,  C12N9/93

摘要： Compositions and methods of producing vaccines, including methods wherein whole organism vaccines are provided with limited replication abilities, thereby increasing vaccine safety and efficacy, through the use of non-natural, unnatural, or non-naturally encoded amino acids.

摘要翻译： 制备疫苗的组合物和方法，包括通过使用非天然的，非天然的或非天然编码的氨基酸使整个生物体疫苗具有有限的复制能力，从而增加疫苗安全性和功效的方法。