公开(公告)号：US09433648B2

公开(公告)日：2016-09-06

申请号：US14757765

申请日：2015-12-23

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37 ,  A61K38/00 ,  A61K35/62

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US09017732B1

公开(公告)日：2015-04-28

申请号：US14563829

申请日：2014-12-08

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37 ,  A61K38/00 ,  A01N63/00 ,  A61K35/62

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US09005667B1

公开(公告)日：2015-04-14

申请号：US14552388

申请日：2014-11-24

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37 ,  A61K38/00 ,  A61K31/4745 ,  A61K31/555 ,  A61K35/62

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US10064897B2

公开(公告)日：2018-09-04

申请号：US15426576

申请日：2017-02-07

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman M. Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37 ,  A61K35/62

摘要： Methods are also provided for isolating and using a whole-saliva leech extract in the treatment of a bacterial skin infection. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

公开(公告)号：US20160120913A1

公开(公告)日：2016-05-05

申请号：US14757765

申请日：2015-12-23

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/62 ,  A61K31/555 ,  A61K45/06 ,  A61K31/4745

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

公开(公告)号：US20140356448A1

公开(公告)日：2014-12-04

申请号：US14282865

申请日：2014-05-20

申请人： Biopep Solutions, Inc.

发明人： ABBAS MOHAMMAD GHAWI ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/62 ,  A61K31/282 ,  A61K31/4745

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US08784896B2

公开(公告)日：2014-07-22

申请号：US13624860

申请日：2012-09-21

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohammad Alaama

IPC分类号： A61K35/37

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US09480720B1

公开(公告)日：2016-11-01

申请号：US15222180

申请日：2016-07-28

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37 ,  A61K38/00 ,  A61K35/62

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US08790711B2

公开(公告)日：2014-07-29

申请号：US13624856

申请日：2012-09-21

申请人： Biopep Solutions, Inc.

发明人： Abbas Mohammad Ghawi ,  Ahmed Merzouk ,  Abdualrahman Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。

公开(公告)号：US08685462B1

公开(公告)日：2014-04-01

申请号：US13624847

申请日：2012-09-21

申请人： Biopep Solutions, Inc.

发明人： Ahmed Merzouk ,  Abbas Mohammad Ghawi ,  Abdualrahman M. Abdualkader ,  Mohamed Alaama

IPC分类号： A61K35/37

CPC分类号： A61K35/62 ,  A01K67/033 ,  A01N63/00 ,  A61K9/0014 ,  A61K31/282 ,  A61K31/4745 ,  A61K31/555 ,  A61K38/21 ,  A61K45/06 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/393 ,  Y02A50/40 ,  Y02A50/401 ,  Y02A50/402 ,  Y02A50/406 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/415 ,  Y02A50/419 ,  Y02A50/422 ,  Y02A50/423 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/467 ,  Y02A50/469 ,  Y02A50/47 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/479 ,  Y02A50/481 ,  Y02A50/483 ,  Y02A50/491 ,  A61K2300/00

摘要： Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.

摘要翻译： 提供了分离和使用全唾液水蛭提取物的方法。 所述方法可以包括向水蛭喂养吞噬剂; 诱导水蛭反流，诱导包括将水蛭放置在温度低于约0℃的环境中; 并在冷水蛭的反流中收集未精制的全唾液。 所述方法可以包括通过在约5℃至约40℃的温度范围内升温来振兴水蛭。还提供了稳定的，冻干的全唾液提取物，所述提取物在储存时具有稳定的活性 在低于约-20℃的温度下使用，提取物保持至少6个月的活性的至少70％。 提取物可用于治疗实体瘤，治疗液体肿瘤，治疗糖尿病，治疗病毒性疾病，治疗寄生虫病，治疗抗菌疾病或用作抗氧化剂。