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1.发明申请公开(公告)号：US20020187493A1
公开(公告)日：2002-12-12
申请号：US10079043
申请日：2002-02-20
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12Q1/6858 , C12Q2565/627 , C12Q2523/319
摘要： The invention relates to a method of a mass-spectrometric analysis of known mutation sites in the genome, such as single nucleotide polymorphisms (SNPs), using the method of restricted primer extension. The invention consists of the use of primers with a photocleavable linker. The linker creates a gap in a DNA strand which is almost the same size as a natural DNA building block (nucleoside). The linker forms a bridge over a base pair without inhibiting hybridization or enzymatic extension. However, the linker allows the primers to be cleaved after extension in order to obtain short DNA fragments which can be more easily detected on the mass spectrometer.
摘要翻译： 本发明涉及使用限制性引物延伸的方法对基因组中已知突变位点进行质谱分析的方法，例如单核苷酸多态性（SNP）。 本发明包括使用具有光可透洗接头的引物。 接头在DNA链中产生与天然DNA构建块（核苷）几乎相同大小的间隙。 连接体在碱基对上形成桥，而不抑制杂交或酶促延伸。 然而，接头允许引物在延伸后被切割，以获得在质谱仪上更容易检测到的短DNA片段。
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公开(公告)号：US20020185593A1
公开(公告)日：2002-12-12
申请号：US10132065
申请日：2002-04-25
发明人： Hans-Rudiger Doring
IPC分类号： H01J049/40
CPC分类号： G01N27/622
摘要： An ion mobility spectrometer (IMS) is provided that has a non-radioactive electron source in an evacuated chamber that is separated from the reaction chamber of the IMS by an x-ray window. Electrons from the source impinge upon an x-ray anode, causing the emission of x-ray radiation toward the window. A current controller is provided by which currents in the electron-source chamber are monitored and controlled using a microprocessor circuit. If a maximum permissible residual gas pressure is exceeded, the electron source is automatically shut down and a gettering process is activated.
摘要翻译： 提供了离子迁移谱仪（IMS），其在通过X射线窗从IMS的反应室分离的真空室中具有非放射性电子源。 来自源的电子撞击x射线阳极，导致向窗口发射x射线辐射。 提供电流控制器，通过该电流控制器利用微处理器电路监测和控制电子源室中的电流。 如果超过允许的最大允许残余气体压力，则自动关闭电子源并激活吸气过程。
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公开(公告)号：US6100521A
公开(公告)日：2000-08-08
申请号：US115370
申请日：1998-07-14
CPC分类号： G01N27/622
摘要： An ion mobility spectrometer (IMS) with electric drift fields directed at a central detector electrode. The IMS is rotationally symmetrical and has a disk shape.
摘要翻译： 具有指向中央检测器电极的电漂移场的离子迁移谱仪（IMS）。 IMS是旋转对称的并且具有盘形状。
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公开(公告)号：US6064070A
公开(公告)日：2000-05-16
申请号：US115776
申请日：1998-07-14
申请人： Roland Schnurpfeil , Stefan Klepel
发明人： Roland Schnurpfeil , Stefan Klepel
CPC分类号： G21G4/04 , H01J27/02 , G01N27/622
摘要： The invention relates to a radioactive ion source for generation of low-energy .alpha. or .beta. radiation. Both the activity of the source as well as the range of radiation can be adjusted to the respective application. Due to a carrier layer of semiconductive material, release of radioactive substances is prevented.
摘要翻译： 本发明涉及用于产生低能α或β辐射的放射性离子源。 源的活动以及辐射范围都可以调整到各自的应用。 由于半导体材料的载体层，防止了放射性物质的释放。
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5.发明授权公开(公告)号：US07160680B2
公开(公告)日：2007-01-09
申请号：US10079043
申请日：2002-02-20
CPC分类号： C12Q1/6858 , C12Q2565/627 , C12Q2523/319
摘要： The invention relates to a method of a mass-spectrometric analysis of known mutation sites in the genome, such as single nucleotide polymorphisms (SNPs), using the method of restricted primer extension. The invention consists of the use of primers with a photocleavable linker. The linker creates a gap in a DNA strand which is almost the same size as a natural DNA building block (nucleoside). The linker forms a bridge over a base pair without inhibiting hybridization or enzymatic extension. However, the linker allows the primers to be cleaved after extension in order to obtain short DNA fragments which can be more easily detected on the mass spectrometer.
摘要翻译： 本发明涉及使用限制性引物延伸的方法对基因组中已知突变位点进行质谱分析的方法，例如单核苷酸多态性（SNP）。 本发明包括使用具有光可透洗接头的引物。 接头在DNA链中产生与天然DNA构建块（核苷）几乎相同大小的间隙。 连接体在碱基对上形成桥，而不抑制杂交或酶促延伸。 然而，接头允许引物在延伸后被切割，以获得在质谱仪上更容易检测到的短DNA片段。
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公开(公告)号：US5968837A
公开(公告)日：1999-10-19
申请号：US815421
申请日：1997-03-11
CPC分类号： H01J49/162 , G01N27/622 , Y10T436/163333 , Y10T436/173845 , Y10T436/182 , Y10T436/21 , Y10T436/24
摘要： In a method for photo-ionization ion mobility spectrometry, a reagent gas is added, in particular an aromatic compound, which has a large ionization cross section in the range of ionizing VUV radiation, but a low probability for the formation of protonated quasi-molecular ions. In this way, the detection of only weakly proton affine substances is also amplified or even made possible at all, and also the detection of electronegative substances in a negative operating mode is improved. Preferred reagent gases are benzene, toluene and xylene. FIG. 1
摘要翻译： 在光电离离子迁移谱方法中，添加反应气，特别是在电离VUV辐射范围内具有大离子化截面的芳香族化合物，但形成质子化准分子的可能性低 离子。 这样，只有弱质子仿射物质的检测也被放大甚至成为可能，并且还提高了在负操作模式下检测电负性物质。 优选的试剂气体是苯，甲苯和二甲苯。 图。
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7.发明申请Ion mobility spectrometer with mechanically stabilized vacuum-tight x-ray window 有权具有机械稳定的真空密封X射线窗口的离子迁移谱仪公开(公告)号：US20020185594A1
公开(公告)日：2002-12-12
申请号：US10132066
申请日：2002-04-25
发明人： Hans-Rudiger Doring
IPC分类号： H01J049/40
CPC分类号： G01N27/622 , H01J49/40 , Y10T436/24 , Y10T436/25875
摘要： An ion mobility spectrometer (IMS) has a non-radioactive electron source and an x-ray anode in an evacuated chamber. The impinging of electrons from the source on the anode results in the generation of x-ray radiation. The x-ray radiation passes through a window that provides a vacuum barrier between the electron source chamber and a reaction chamber of the IMS by an x-ray window. A support grid is attached to the reaction-chamber side of the x-ray window, and mechanically stabilizes the window.
摘要翻译： 离子迁移谱仪（IMS）在真空室中具有非放射性电子源和x射线阳极。 来自阳极的电子的入射导致X射线辐射的产生。 X射线辐射通过窗口，其通过X射线窗口在电子源室和IMS的反应室之间提供真空阻挡层。 支撑栅格连接到x射线窗口的反应室侧，并机械地使窗户稳定。
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8.发明授权Method for trace detection by solvent-assisted introduction of substances into an ion mobility spectrometer 有权通过溶剂辅助将物质引入离子迁移谱仪进行痕量检测的方法
公开(公告)号：US6144029A
公开(公告)日：2000-11-07
申请号：US248785
申请日：1999-02-12
申请人： Joachim Adler
发明人： Joachim Adler
CPC分类号： G01N27/622 , G01N1/22 , G01N2001/022
摘要： The invention relates to a method and an ion mobility spectrometer (IMS) for the detection of contaminations by explosives, drugs, wood preservatives or the like. The substance traces collected on a carrier material, e.g. cellulose, are dissolved in a solvent for detection using an IMS and are vaporized together with this solvent from a sample dispenser in the reaction compartment of the IMS. In this way, the sample dispenser can be operated at a considerably lower temperature.
摘要翻译： 本发明涉及用于检测爆炸物，药物，木材防腐剂等污染物的方法和离子迁移谱仪（IMS）。 收集在载体材料上的物质痕迹，例如 纤维素，使用IMS溶解在用于检测的溶剂中，并与来自IMS的反应室中的样品分配器的该溶剂一起蒸发。 以这种方式，样品分配器可以在相当低的温度下操作。
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公开(公告)号：US6107624A
公开(公告)日：2000-08-22
申请号：US115341
申请日：1998-07-14
CPC分类号： G01N27/622
摘要： The invention relates to a switchable ion mobility spectrometer (IMS) with ring electrodes divided into half-rings, which surround the drift chamber of the IMS. In a first operating condition, the two half-rings of each ring electrode are at the same potential and the various ring electrodes are at different potential varying monotonously along the drift path. In this way, ions are transported in the conventional manner along the drift path. In a second operating mode, the half-rings of the ring electrodes are at different potentials, by which the ions are each deflected toward one of the half-rings. The currents thus resulting are captured by the corresponding half-rings, amplified and an ion mobility spectrum is produced. A favorable design for the IMS can be used in many different modes of operation.
摘要翻译： 本发明涉及一种可切换的离子迁移谱仪（IMS），其具有分为半环的环形电极，其环绕IMS的漂移室。 在第一操作条件下，每个环形电极的两个半环处于相同的电位，并且各种环形电极处于沿漂移路径单调变化的不同电位。 以这种方式，沿着漂移路径以常规方式输送离子。 在第二操作模式中，环形电极的半环处于不同的电位，离子各自偏向一个半环。 由此产生的电流被相应的半环捕获，被放大并产生离子迁移谱。 IMS的有利设计可以用于许多不同的操作模式。
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公开(公告)号：US6023169A
公开(公告)日：2000-02-08
申请号：US890400
申请日：1997-07-09
摘要： ECD with a non-radioactive electron source for generation of ions in a reaction chamber. The reaction chamber consists of two partial chambers, of which one is evacuated and contains the electron source, and the other contains connections for the feeding in of analysis gas as well as a collector electrode for detecting ions. The partition between the two partial chambers is permeable for electrons from the source but impermeable for gas molecules. The electron source may be a thermoemitter or a photocathode, which is irradiated from the outside through a window. In this way, contact between the analysis substance and the electron source is prevented, which increases measurement stability.
摘要翻译： 具有用于在反应室中产生离子的非放射性电子源的ECD。 反应室由两个部分室组成，其中一个被抽真空并且包含电子源，另一个包含用于分析气体进料的连接以及用于检测离子的集电极。 两个部分室之间的分隔物对于来自源的电子是可渗透的，但是对于气体分子是不可渗透的。 电子源可以是热电偶或光电阴极，其通过窗口从外部照射。 以这种方式，防止分析物质与电子源之间的接触，这增加了测量的稳定性。
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