-
公开(公告)号：US11179909B2
公开(公告)日：2021-11-23
申请号：US16829399
申请日：2020-03-25
IPC分类号： B30B9/12 , C02F11/125 , B30B9/14 , B30B9/18
摘要： A method for removing liquid from a slurry includes: (i) feeding the slurry into the inlet of a press; (ii) removing liquid from the slurry as the slurry advances through a deliquifying section of the press, thereby producing a pressate stream; (iii) forming a plug of material as the slurry is advanced through a plug formation section of the press; (iv) advancing the plug of material to a discharge section of the press; and (v) removing additional liquid from the plug of material by operating the press so that the pressure in the discharge section of the press is greater than the pressure of the pressate stream by at least 2 psi. The present invention also relates to a method for controlling discharge consistency from a press, without provision of discharge restrictors, by varying the pressure differential between a discharge section of the press and a pressate stream.
-
公开(公告)号：US10766826B2
公开(公告)日：2020-09-08
申请号：US16229475
申请日：2018-12-21
发明人： Patrick J. Foody , Robert Glenns
IPC分类号： C05D9/00 , C09K17/40 , C05G3/80 , C12F3/10 , C05G1/00 , C05F5/00 , C05C11/00 , C13K1/02 , C05C3/00 , C13K13/00
摘要： The present invention relates to a method for producing a composition for use in land application. The method comprises: (a) obtaining a fermentation product by a production process comprising the steps of: (i) treating lignocellulosic feedstock to produce sugar; (ii) fermenting the sugar to produce a fermented mixture comprising the fermentation product; and (iii) recovering the fermentation product from the fermented mixture in one or more stages to produce a concentrated fermentation product and still bottoms; and(b) recovering the still bottoms, the still bottoms comprising organic and inorganic components; and (c) providing the still bottoms for use in a land application. Also provided is a soil conditioning composition for use in land application. The soil conditioning composition contains still bottoms and optionally other components.
-
公开(公告)号：US10364422B2
公开(公告)日：2019-07-30
申请号：US15005309
申请日：2016-01-25
发明人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
摘要： Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.
-
公开(公告)号：US09834804B2
公开(公告)日：2017-12-05
申请号：US14353916
申请日：2012-12-12
发明人： Philippe J. Dufresne , Adam H. Colville , Barbara Fryzuk , Loreta Gudynaite-Savitch , Christopher M. D. Hindle , Boguslaw Ploch , John J. Tomashek
IPC分类号： C12N1/14 , C12N9/24 , C12P19/14 , C07K14/37 , C07K14/38 , C12P19/00 , C12N9/14 , C12N9/42 , C12P19/02
CPC分类号： C12P19/14 , C07K14/37 , C07K14/38 , C12N1/14 , C12N9/14 , C12N9/24 , C12N9/2437 , C12P19/00 , C12P19/02
摘要： The present invention provides an isolated fungal cell that is capable of producing one or more biomass-degrading enzymes and that exhibits increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. Also provided is a fermentation processes for producing one or more biomass-degrading enzymes comprising a fungal cells exhibiting increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. The biomass-degrading enzymes produced by the isolate fungal cell and fermentation processes of the present invention may be used in a process to produce soluble sugars from biomass.
-
公开(公告)号：US09206406B2
公开(公告)日：2015-12-08
申请号：US13575046
申请日：2011-02-11
CPC分类号： C12N9/2437 , C07K2319/20 , C12N9/2402 , C12N9/2445 , C12P7/06 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolyzing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolyzing cellulose or hemicellulose in the presence of lignin.
摘要翻译： 提供了一种修饰的1族碳水化合物结合模块（CBM），其包含位置10,11,12,14,17,21,24,29,31,33和37中的一个或多个的氨基酸取代，所述位置由对准确定 具有SEQ ID NO：30的家族1 CBM氨基酸序列，并且与SEQ ID NO：30显示约50％至约99.9％的氨基酸序列同一性。还提供了包含修饰的家族1 CBM，遗传 构建体和用于表达经修饰的家族1 CBM或修饰的糖苷酶的转基因微生物。 修饰的家族1 CBM在木质素存在下赋予木质素结合降低和/或水解活性增加至修饰的糖苷酶，其可用于在木质素存在下水解纤维素或半纤维素的方法。
-
6.发明授权公开(公告)号：US08759049B2
公开(公告)日：2014-06-24
申请号：US13032127
申请日：2011-02-22
摘要： The present invention relates to a method for producing a fermentation product from a sugar hydrolysate. The method comprises fermenting the sugar hydrolysate in a fermentation system with yeast to produce a fermentation broth comprising a fermentation product; introducing acid and an oxidant, such as chlorine dioxide, to the fermentation system so as to expose microbial contaminants in the fermentation system at one or more stages to chlorine dioxide and a pH of less than 3.0; and recovering the fermentation product. In one example of the invention, a yeast slurry obtained from a yeast recycle step is treated with acid and the oxidant.
摘要翻译： 本发明涉及从糖水解产物生产发酵产物的方法。 该方法包括在发酵系统中用酵母发酵糖水解产物以产生包含发酵产物的发酵液; 将酸和氧化剂（例如二氧化氯）引入发酵系统，以便在一个或多个阶段将发酵系统中的微生物污染物暴露于二氧化氯和小于3.0的pH; 并回收发酵产物。 在本发明的一个实例中，从酵母循环步骤获得的酵母浆液用酸和氧化剂处理。
-
7.发明申请MODIFIED YEAST STRAINS EXHIBITING ENHANCED FERMENTATION OF LIGNOCELLULOSIC HYDROLYSATES 审中-公开改良的YEAST菌株展示增强水杨酸的发酵公开(公告)号：US20140030795A1
公开(公告)日：2014-01-30
申请号：US14045278
申请日：2013-10-03
发明人： Jennifer Donaldson , Jan-Maarten A. Geertman , Glenn D. Munkvold , Gary Pigeau , Philippe J. Dufresne , Loreta Gudynaite-Savitch
IPC分类号： C12N15/81
摘要： The present invention relates to novel xylose-fermenting yeast strains (for example, yeast of the genus Saccharomyces, e.g., S. cerevisiae) with an enhanced ability to ferment the xylose (and/or another pentose sugar) present in a lignocellulosic hydrolysate to a fermentation product(s) (for example, an alcohol (e.g., ethanol) or a sugar alcohol (e.g., xylitol)).
摘要翻译： 本发明涉及具有增强木质纤维素水解产物中存在的木糖（和/或其他戊糖）的能力的新型木糖发酵酵母菌株（例如酿酒酵母属酵母，例如酿酒酵母） 发酵产物（例如，醇（例如乙醇）或糖醇（例如木糖醇））。
-
公开(公告)号：US20130052694A1
公开(公告)日：2013-02-28
申请号：US13593735
申请日：2012-08-24
申请人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
发明人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N15/80 , C12P19/14
摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolysing a cellulosic substrate with such cellulase enzyme mixtures.
摘要翻译： 提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。 还提供用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。
-
公开(公告)号：US20120325203A1
公开(公告)日：2012-12-27
申请号：US13522069
申请日：2011-02-08
申请人： Robert Griffin , Vladimir Pavlovic , Daphne Wahnon
发明人： Robert Griffin , Vladimir Pavlovic , Daphne Wahnon
IPC分类号： C13K13/00
CPC分类号： C13K1/02 , C08H6/00 , C08H8/00 , C12P2201/00 , D21C5/005 , D21C9/008 , D21C9/083 , Y02E50/16
摘要： A method for processing of a lignocellulosic feedstock is provided comprising exposing lignocellulosic feedstock to acid in a reactor to hydrolyze at least hemicellulose present in said lignocellulosic feedstock, wherein during said exposing, a scale deposit comprising lignin forms on the inner surface of said reactor; and treating the scale deposit with an alkali solution at a temperature between about 140° C. and about 250° C. so as to remove scale. Also provided is a method for reducing scale deposit that forms on process equipment during a stage of reacting a lignocellulosic feedstock with acid.
摘要翻译： 提供了一种用于处理木质纤维素原料的方法，包括在反应器中将木质纤维素原料暴露于酸中以至少水解所述木质纤维素原料中存在的半纤维素，其中在所述暴露期间，在所述反应器的内表面上包含木质素形式的垢沉积物; 并在约140℃至约250℃之间的温度下用碱溶液处理垢垢，以除去垢。 还提供了一种用于在使木质纤维素原料与酸反应的阶段期间减少在工艺设备上形成的垢沉积物的方法。
-
10.发明申请METHOD FOR THE PRODUCTION OF A FERMENTATION PRODUCT FROM A PRETREATED LIGNOCELLULOSIC FEEDSTOCK 失效从预先准备好的发酵饲料生产发酵产品的方法公开(公告)号：US20120252085A1
公开(公告)日：2012-10-04
申请号：US13466417
申请日：2012-05-08
IPC分类号： C12P7/14
摘要： A method for obtaining a fermentation product from a sugar hydrolysate obtained from a feedstock containing hemicellulose, by (i) removing suspended fiber solids from said sugar hydrolysate to obtain a clarified sugar solution; (ii) fermenting xylose in the clarified sugar solution in a fermentation reaction with yeast to produce a fermentation broth comprising the fermentation product; (iii) separating the yeast from the fermentation broth to produce a yeast slurry; (vi) treating the yeast slurry thus obtained with an oxidant to kill microbial contaminants, thereby an oxidant-treated yeast slurry; (v) re-introducing at least a portion of the oxidant-treated yeast back to step (ii) to increase the concentration of yeast in said fermentation reaction; and (vi) recovering the fermentation product.
摘要翻译： 一种从含有半纤维素的原料获得的糖水解产物获得发酵产物的方法，通过（i）从所述糖水解产物中除去悬浮的纤维固体以获得澄清的糖溶液; （ii）在与酵母的发酵反应中在澄清的糖溶液中发酵木糖以产生包含发酵产物的发酵液; （iii）将酵母与发酵液分离以产生酵母浆液; （vi）用氧化剂处理由此获得的酵母浆料以杀死微生物污染物，由此进行氧化剂处理的酵母浆料; （v）将至少一部分经氧化剂处理的酵母重新引入步骤（ii）以增加所述发酵反应中酵母的浓度; 和（vi）回收发酵产物。
-
-
-
-
-
-
-
-
-
搜索结果
120 条记录 (耗时 0.034 秒)
