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15 条记录 (耗时 0.014 秒)

    Microfluidic cellular device and methods of use thereof
    1.
    发明授权
    Microfluidic cellular device and methods of use thereof 有权

    公开(公告)号：US11426724B2

    公开(公告)日：2022-08-30

    申请号：US16244920

    申请日：2019-01-10

    申请人： NanoCav, LLC

    发明人： Yu-Chun Kung

    IPC分类号： B01L3/00 ,  G01N1/40 ,  G01N15/02

    摘要： Systems and methods for using microfluidic devices to concentrate cells, to perform buffer changes, to sort cells based on size, and/or to isolate particular types of cells in a rapid manner, are presented. Cells flow into a matrix of posts, wherein the posts are distributed along diagonal lines in the chamber. The cells are deflected in a lateral manner, towards a side of a chamber and are collected upon exiting the chamber.

    Devices and methods for mitochondria replacement and for generating cellular therapeutics
    3.
    发明授权
    Devices and methods for mitochondria replacement and for generating cellular therapeutics 有权

    公开(公告)号：US11639510B2

    公开(公告)日：2023-05-02

    申请号：US16517017

    申请日：2019-07-19

    申请人： NanoCav, LLC

    发明人： Ting-Hsiang Sherry Wu ,  Artin Mehrabi ,  Jon Thomas Van Lew

    IPC分类号： C12N15/87 ,  C12N5/0775 ,  B01L3/00

    摘要： Devices and methods are presented for delivery of various macrostructures into cells, such as depleted cells as well as methods of generating engineered cells using said devices and methods. Cells are placed on a porous membrane. A force is applied by a configurable actuator to a deformable fluid reservoir that generates an applied pressure to macrostructures in a solution, causing the macrostructures to pass through the porous membrane and triggering uptake of at least some of the macrostructures into the cells to form transfected cells. Said devices and methods may be used in a process to replace defective endogenous mtDNA with corrected mtDNA to generate cellular-based therapeutics for administration to a patient. The customizable actuator may be configured with parameters to optimize efficiency for a given cell type and material to be transfected. Machine learning techniques also may be utilized to optimize transfection parameters.

    Mechanical transfection devices and methods
    6.
    发明授权
    Mechanical transfection devices and methods 有权

    公开(公告)号：US11046976B2

    公开(公告)日：2021-06-29

    申请号：US16139722

    申请日：2018-09-24

    申请人： NanoCav, LLC

    发明人： Ting Wu

    IPC分类号： C12N15/87

    摘要： Systems and methods for transfection devices are contemplated for delivery of various complex macrostructures. Preferred systems and methods are suitable for mRNA reprogramming and genome editing and use mechanical force to induce uptake of the macrostructures in a target cell. Contemplated devices are able to achieve high throughput of transfected cells in remarkably short time that remain viable and are capable of producing colonies.

    Micro flow-through electroporation devices and methods of cell transfection
    8.
    发明授权
    Micro flow-through electroporation devices and methods of cell transfection 有权

    公开(公告)号：US11377652B2

    公开(公告)日：2022-07-05

    申请号：US16506190

    申请日：2019-07-09

    申请人： NanoCav, LLC

    发明人： Chih-Wei Chang

    IPC分类号： C12N15/87 ,  C12N13/00

    摘要： Systems and methods are provided for transfecting cells, such as mammalian cells and nonmammalian cells, using an electroporation apparatus having an electroporation chamber including an upper micromesh electrode, a lower micromesh electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of cells into the electroporation chamber and a first output allowing passage of electroporated cells from the electroporation chamber. The first input and the first output are separated by an offset distance.

    Micro Flow-Through Electroporation Devices And Methods Of Cell Transfection
    9.
    发明申请
    Micro Flow-Through Electroporation Devices And Methods Of Cell Transfection 有权

    公开(公告)号：US20210348155A1

    公开(公告)日：2021-11-11

    申请号：US17380799

    申请日：2021-07-20

    申请人： NanoCav, LLC

    发明人： Chih-Wei CHANG

    IPC分类号： C12N13/00 ,  C12N15/87

    摘要： Systems and methods are provided for transfecting cells, such as mammalian cells and nonmammalian cells, using an electroporation apparatus having an electroporation chamber including an upper micromesh electrode, a lower micromesh electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of cells into the electroporation chamber and a first output allowing passage of electroporated cells from the electroporation chamber. The first input and the first output are separated by an offset distance.

    Mechanical transfection devices and methods
    10.
    发明授权
    Mechanical transfection devices and methods 有权

    公开(公告)号：US10760040B1

    公开(公告)日：2020-09-01

    申请号：US14754625

    申请日：2015-06-29

    申请人： NanoCav, LLC

    发明人： Ting Wu

    IPC分类号： C12M1/12 ,  C12M1/00 ,  C12M1/34

    摘要： A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.