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公开(公告)号：US20220120725A1
公开(公告)日：2022-04-21
申请号：US17502064
申请日：2021-10-15
发明人： Peiwu LI , Qi ZHANG , Huali XIE , Xiupin WANG , Xiaofeng YUE , Yizhen BAI
摘要： The present invention relates to an aflatoxin contamination risk warning molecule and use thereof. The steps are as follows: weighing a quantitative sample, extracting the aflatoxin contamination risk warning molecule to obtain a sample extract, and detecting and analyzing the sample extract to obtain a quantitative result of the aflatoxin contamination risk warning molecule; performing modeling with a chemometrics method using the content of one or more of the aflatoxin contamination risk warning molecules as a variable to obtain a classification prediction model, and performing risk assessment on aflatoxin contamination risk of the sample based on the classification prediction model, wherein a warning molecule of an aflatoxin toxigenic strain is one or a combination of more than one of versiconol (VOH), versicolorin B (Ver B), and 5-methoxysterigmatocystin (5-MST).
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公开(公告)号：US11033032B2
公开(公告)日：2021-06-15
申请号：US16204654
申请日：2018-11-29
发明人： Peiwu Li , Xiaoxia Ding , Yizhen Bai , Wen Zhang , Qi Zhang
摘要： The present invention relates to the microbiological field, and particularly relates to a Leclercia adcarboxglata biocontrol strain efficiently inhibiting production of aflatoxins by Aspergillus flavus. The Leclercia adcarboxglata biocontrol strain Wt16 was deposited at China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, and the accession number of the strain is CCTCC No. M2017331. The Leclercia adcarboxglata strain Wt16 is isolated from peanut pods for the first time which can significantly inhibit aflatoxins production by Aspergillus flavus, and also has an extremely good effect on inhibiting aflatoxins production by Aspergillus flavus for peanuts from different sources.
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公开(公告)号：US20210132066A1
公开(公告)日：2021-05-06
申请号：US16492303
申请日：2018-03-07
发明人： Zhaowei ZHANG , Peiwu LI , Qi ZHANG , Du WANG , Wen ZHANG
IPC分类号： G01N33/577 , G01N21/64 , G01N33/558 , G01N33/53
摘要： A time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and an application thereof are disclosed. The kit comprises a time-resolved fluorescent immunochromatographic test strip and sample reaction vials each containing a europium-labeled monoclonal antibody lyophilized product; wherein the fluorescent test strip comprises a PVC substrate, and a surface of the PVC substrate is adhered with a water absorbing pad (1), a detection pad (2) and a sample pad (3) from top to bottom, adjacent pads being connected and overlapping at connections. The detection pad (2) adopts a nitrocellulose membrane as the base thereof and is arranged with a lateral quality control line (5) and five detection lines (5, 6, 7, 8, 9) from top to bottom each covered by a bovine serum albumin conjugate of each toxin. The fumonisin B1 monoclonal antibody is generated by the hybridoma cell strain Fm7A11 having China Center for Type Culture Collection (CCTCC) accession number C201636. The kit is applicable to simultaneous detection of mixed contamination of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and sterigmatocystin.
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4.发明申请公开(公告)号：US20200291491A1
公开(公告)日：2020-09-17
申请号：US16886791
申请日：2020-05-29
发明人： Qi ZHANG , Peiwu LI , Tong WANG , Xiaoxia DING
摘要： The invention belongs to the field of microbes and specifically relates to a myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and an application thereof. A myroides odoratimimus biocontrol strain 3J2MO is preserved at the China Center for Type Culture Collection (referred to as CCTCC) on Jun. 13, 2017 with preservation number CCTCC No. M 2017329. The myroides odoratimimus biocontrol strain of the invention can effectively degrade aflatoxin and may be configured to degrade aflatoxin and treat aflatoxin pollution of food crops.
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公开(公告)号：US11635429B2
公开(公告)日：2023-04-25
申请号：US17777270
申请日：2020-11-16
发明人： Xiaoqian Tang , Du Wang , Jun Jiang , Qi Zhang , Peiwu Li
IPC分类号： G01N33/543 , G01N33/531
摘要： An immunochromatography test strip for detecting pollution of diacetoxyscirpenol includes a bottom plate, where a water absorption pad, a detection pad, a gold-labeled pad and a sample pad are sequentially attached to one side of the bottom plate from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane, the quality control line is coated with a rabbit antimouse polyclonal antibody, the detection line is located below the quality control line, and the detection line is coated with a diacetoxyscirpenol-ovalbumin conjugate; the gold-labeled pad is transversely spray-coated with a nanogold-labeled anti-diacetoxyscirpenol monoclonal antibody; and the anti-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO.C201881.
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公开(公告)号：US20220380796A1
公开(公告)日：2022-12-01
申请号：US17624173
申请日：2020-07-01
申请人： OIL CROPS RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES , SYNGENTA CROP PROTECTION AG , SYNGENTA BIOTECHNOLOGY CHINA CO, LTD
发明人： Zhihui SHAN , Qingnan HAO , Haifeng CHEN , Yanyan YANG , Chanjuan ZHANG , Limiao CHEN , Songli YUAN , Dong CAO , Wei GUO , Xiaojuan ZHANG , Shuilian CHEN , Zhonglu YANG , Dezhen QIU , Xinan ZHOU , Qingli LIU , Becky Welsh BREITINGER , Shujie DONG
IPC分类号： C12N15/82 , C12Q1/6895 , C07K14/415
摘要： The present invention provides methods and compositions for identifying, selecting, and/or producing a soybean plant or germplasm resistant to Asian soybean rust using markers, genes and chromosomal intervals derived from Glycine max strain SX6907. Asian soybean rust resistant soybean seeds, plants, and germplasms are also provided.
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公开(公告)号：US20220120722A1
公开(公告)日：2022-04-21
申请号：US17502062
申请日：2021-10-15
发明人： Qi ZHANG , Peiwu LI , Huali XIE , Xiupin WANG , Xiaofeng YUE , Wen ZHANG
摘要： The present invention relates to an early warning method before the occurrence of aflatoxin contamination. The steps are as follows: extracting toxins from the sample to obtain a sample extract, and subjecting the sample extract to detection and analysis by liquid chromatography-high resolution mass spectrometer, performing qualitative analysis based on the mass spectrometry information to obtain qualitative results, performing quantitative analysis based on a standard curve of the chromatographic peak area of each warning molecule/the peak area of the internal standard-warning molecule concentration to obtain quantitative results of these warning molecules, wherein a risk of aflatoxin contamination of the sample is assessed to obtain a classification prediction model, inputting the quantitative results of the warning molecules for a toxigenic strain of Aspergillus flavus, and outputting a risk assessment result based on the classification prediction model, thereby achieving the early warning before aflatoxin contamination occurs.
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8.发明授权公开(公告)号：US11622560B2
公开(公告)日：2023-04-11
申请号：US16886791
申请日：2020-05-29
发明人： Qi Zhang , Peiwu Li , Tong Wang , Xiaoxia Ding
摘要： The invention belongs to the field of microbes and specifically relates to a myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and an application thereof. A myroides odoratimimus biocontrol strain 3J2MO is preserved at the China Center for Type Culture Collection (referred to as CCTCC) on Jun. 13, 2017 with preservation number CCTCC No. M 2017329. The myroides odoratimimus biocontrol strain of the invention can effectively degrade aflatoxin and may be configured to degrade aflatoxin and treat aflatoxin pollution of food crops.
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公开(公告)号：US20220412966A1
公开(公告)日：2022-12-29
申请号：US17777269
申请日：2020-11-16
IPC分类号： G01N33/533 , G01N33/577
摘要： A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate. The three mycotoxins including 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin can be rapidly and synchronously detected.
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公开(公告)号：US20220412965A1
公开(公告)日：2022-12-29
申请号：US17777268
申请日：2020-11-16
发明人： Qi ZHANG , Xiaoqian TANG , Yizhen BAI , Wen ZHANG , Peiwu LI
IPC分类号： G01N33/533 , G01N33/558 , G01N33/577
摘要： A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.
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