METHOD FOR DETECTING AND QUNANTIFYING ENDOGENOUS WHEAT DNA SEQUENCE

    公开(公告)号:US20120009586A1

    公开(公告)日:2012-01-12

    申请号:US13218398

    申请日:2011-08-25

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C12Q1/6895

    摘要: It is an object of the present invention to provide partial sequences of endogenous wheat DNA (genome) which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in PCR, and to provide primers for amplifying these partial sequences, along with a good method for detecting and quantifying endogenous DNA using these primers. The present invention provides, in a method for detecting or quantifying an endogenous wheat DNA sequence in a test sample by means of a polymerase chain reaction, a method comprising a step of using a nucleic acid in the test sample or a nucleic acid extracted from the test sample as a template to amplify the nucleic acid of a region comprising at least 80% or more of a nucleotide sequence represented by one of SEQ ID NO:1 through SEQ ID NO:7 using a primer pair capable of amplifying that region, and a step of detecting or quantifying the amplified nucleic acid.

    METHOD FOR DETECTING AND QUNANTIFYING ENDOGENOUS WHEAT DNA SEQUENCE
    4.
    发明申请
    METHOD FOR DETECTING AND QUNANTIFYING ENDOGENOUS WHEAT DNA SEQUENCE 失效
    检测和调控内源性DNA序列的方法

    公开(公告)号:US20120009585A1

    公开(公告)日:2012-01-12

    申请号:US13218385

    申请日:2011-08-25

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C12Q1/6895

    摘要: It is an object of the present invention to provide partial sequences of endogenous wheat DNA (genome) which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in PCR, and to provide primers for amplifying these partial sequences, along with a good method for detecting and quantifying endogenous DNA using these primers. The present invention provides, in a method for detecting or quantifying an endogenous wheat DNA sequence in a test sample by means of a polymerase chain reaction, a method comprising a step of using a nucleic acid in the test sample or a nucleic acid extracted from the test sample as a template to amplify the nucleic acid of a region comprising at least 80% or more of a nucleotide sequence represented by one of SEQ ID NO:1 through SEQ ID NO:7 using a primer pair capable of amplifying that region, and a step of detecting or quantifying the amplified nucleic acid.

    摘要翻译: 本发明的目的是提供单拷贝的内源小麦DNA(基因组)的部分序列,并且允许在PCR中不与其它植物交叉反应来特异性检测小麦,并提供用于扩增这些部分序列的引物 ,以及使用这些引物检测和定量内源DNA的良好方法。 本发明在通过聚合酶链式反应检测或定量测试样品中的内源性小麦DNA序列的方法中提供了一种方法,该方法包括使用测试样品中的核酸或从测试样品中提取的核酸 测试样品作为模板,以使用能够扩增该区域的引物对来扩增包含至少80%或更多个由SEQ ID NO:1至SEQ ID NO:7之一表示的核苷酸序列的核酸的核酸的核酸,以及 检测或定量扩增的核酸的步骤。

    METHOD FOR DETECTING AND QUNANTIFYING ENDOGENOUS WHEAT DNA SEQUENCE

    公开(公告)号:US20120009584A1

    公开(公告)日:2012-01-12

    申请号:US13218358

    申请日:2011-08-25

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C12Q1/6895

    摘要: It is an object of the present invention to provide partial sequences of endogenous wheat DNA (genome) which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in PCR, and to provide primers for amplifying these partial sequences, along with a good method for detecting and quantifying endogenous DNA using these primers. The present invention provides, in a method for detecting or quantifying an endogenous wheat DNA sequence in a test sample by means of a polymerase chain reaction, a method comprising a step of using a nucleic acid in the test sample or a nucleic acid extracted from the test sample as a template to amplify the nucleic acid of a region comprising at least 80% or more of a nucleotide sequence represented by one of SEQ ID NO:1 through SEQ ID NO:7 using a primer pair capable of amplifying that region, and a step of detecting or quantifying the amplified nucleic acid.

    Array antenna
    6.
    发明授权
    Array antenna 有权
    阵列天线

    公开(公告)号:US07095384B2

    公开(公告)日:2006-08-22

    申请号:US11134286

    申请日:2005-05-23

    IPC分类号: H01Q21/00 H01Q1/38

    摘要: Antenna elements are arranged in line on a surface of a substrate at specific intervals along a longitudinal direction of the substrate. A phase-inverting distributor is formed on the surface of the substrate in an area located between two antenna elements which are closest to and located on both sides of a vertical centerline, or a reference line, of the substrate. A primary feeder line formed along the longitudinal direction of the substrate extends from the phase-inverting distributor on both left and right sides thereof. The individual antenna elements are connected to the primary feeder line by secondary feeder lines having predetermined impedances. The secondary feeder lines are connected to the respective antenna elements on sides thereof which are perpendicular to the longitudinal direction of the substrate and face the reference line on which the phase-inverting distributor is located.

    摘要翻译: 天线元件沿着衬底的纵向方向以特定间隔沿着衬底表面布置。 相位反转分配器形成在基板表面上的位于最靠近并位于基板的垂直中心线或参考线两侧的两个天线元件之间的区域中。 沿着基板的纵向方向形成的主馈线在其左侧和右侧从相转向分配器延伸。 各个天线元件通过具有预定阻抗的辅助馈线连接到主馈线。 辅助馈电线路连接到垂直于衬底的纵向方向的一侧的相应的天线元件,并且面对相反分配器所在的基准线。

    Image drawing method, image drawing apparatus, recording medium, and program
    7.
    发明授权
    Image drawing method, image drawing apparatus, recording medium, and program 失效
    图像绘制方法,图像绘制装置,记录介质和程序

    公开(公告)号:US06914612B2

    公开(公告)日:2005-07-05

    申请号:US09784852

    申请日:2001-02-16

    CPC分类号: G06T13/60

    摘要: An image drawing technique includes a method of drawing a randomly changing image of an object and a method of drawing a revised image including a shimmering image in a background image. The method of drawing a randomly changing image of an object establishes a drawing pattern of the object, the drawing pattern including a plurality of extraction areas, each extraction area including a portion of the drawing pattern. A portion of the drawing pattern is extracted from one of the extraction areas and is combined with a randomly selected mask pattern to draw a composite image which is output on a monitor. The extracting and combining steps are repeated for a next extraction area located at a predetermined distance in a defined direction from a previous extraction area. The method of drawing a revised image includes randomly altering a portion of the background image to generate an altered image, and combining the altered image and another mask pattern to generate another composite image. The another composite image is combined with a remaining portion of the background image to draw the revised image. The another composite image and the revised image are output for display on a monitor.

    摘要翻译: 图像绘制技术包括绘制对象的随机变化图像的方法以及在背景图像中绘制包括闪烁图像的修正图像的方法。 绘制对象的随机变化的图像的方法建立了对象的绘图模式,该绘制模式包括多个提取区域,每个提取区域包括绘​​图模式的一部分。 从一个提取区域提取绘制图案的一部分,并与随机选择的掩模图案组合以绘制在监视器上输出的合成图像。 针对位于距先前提取区域的规定方向上的预定距离的下一个提取区域重复提取和组合步骤。 绘制修正图像的方法包括随机地改变背景图像的一部分以生成改变的图像,并且将改变的图像和另一掩模图案组合以生成另一合成图像。 另一个合成图像与背景图像的剩余部分组合以绘制修改后的图像。 输出另一张合成图像和修改后的图像显示在显示器上。

    Rice ornithine carbamyltransferase gene, and a vector containing said
gene and a transformant
    8.
    发明授权
    Rice ornithine carbamyltransferase gene, and a vector containing said gene and a transformant 失效
    赖氨酸氨基甲酰转移酶基因,以及含有该基因和转化体的载体

    公开(公告)号:US5928925A

    公开(公告)日:1999-07-27

    申请号:US92025

    申请日:1998-06-05

    摘要: An object of the present invention is to isolate complementary DNA coding for ornithine carbamyltransferase derived from a rice plant, necessary for development of a plant ornithine carbamyltransferase gene mutated to have phaseolotoxin resistance, and further to establish an expression system in microorganisms for simply evaluating the functions of the gene mutated to have resistance. This object is solved by a rice ornithine carbamyltransferase gene coding for a protein consisting of the amino acid sequence shown in SEQ ID NO:6 in the Sequence Listing, a plasmid vector containing the gene, and a transformant carrying the plasmid vector.

    摘要翻译: 本发明的目的是分离编码来自水稻植物的鸟氨酸氨基甲酰基转移酶的互补DNA,其是突变为具有相变毒素抗性的植物鸟氨酸氨基甲酰转移酶基因的开发所必需的,并且进一步建立微生物中的表达系统以简单地评估功能 的基因突变具有抗性。 该目的通过编码由序列表中SEQ ID NO:6所示的氨基酸序列构成的蛋白质的含有鸟氨酸氨基甲酰基转移酶基因,含有该基因的质粒载体和携带质粒载体的转化体来解决。