公开(公告)号：US08092681B2

公开(公告)日：2012-01-10

申请号：US12176492

申请日：2008-07-21

Applicant: François Couillard ,  Alain Foucault ,  Daniel Durand

Inventor： François Couillard ,  Alain Foucault ,  Daniel Durand

IPC: B01D15/08

CPC classification number: G01N21/07 ,  B01D15/1892 ,  G01N30/42

Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (l) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Abstract translation: 本发明涉及一种通过液 - 液离心色谱分离液体进料的成分的方法和用于实施该液体进料的装置。 具有不同分配系数的成分（A，B），使得它们分别以轻溶剂（1）和较重溶剂（L）以不同速度携带，将进料在分离塔（col）的中间点连续注入 ），分别在由串联的至少一组分离单元的互连构成的柱的两端分别进行包括重溶剂注入阶段和轻溶剂注入阶段的连续循环，分离的溶剂（FA， FB）在与溶剂注入点相对的列端部的每个阶段期间收集。

公开(公告)号：US20110048620A1

公开(公告)日：2011-03-03

申请号：US12856081

申请日：2010-08-13

Applicant: Alain FOUCAULT ,  Etienne Juchauld ,  David Marsal ,  Stéphane Otin

Inventor： Alain FOUCAULT ,  Etienne Juchauld ,  David Marsal ,  Stéphane Otin

IPC: B29C53/60

CPC classification number: B29C70/68 ,  B29L2031/08 ,  F01D5/282 ,  F01D5/34 ,  F05D2300/44 ,  F05D2300/603 ,  F05D2300/614 ,  Y02T50/672

Abstract: A first insert (15) forming a ring is placed inside and in contact with a core (10) in degradable material. On the outer face of the core a plurality of inserts (20) is arranged each comprising a platform (21) applied against the outer face of the core and at least one blade (22) extending substantially radially outwards. A fibrous structure (56) is wound around the core passing over the platforms (21) and over the inner surface of the ring-shaped insert (15). Moulding of the fibrous structure, impregnated with a composition containing a resin, is performed before polymerization of the resin and removal of the core.

Abstract translation: 形成环的第一插入件（15）放置在可降解材料的内部并与芯（10）接触。 在芯的外表面上，布置有多个插入件（20），每个插入件包括施加在芯的外表面上的平台（21）和基本上径向向外延伸的至少一个叶片（22）。 纤维结构（56）缠绕在穿过平台（21）并且在环形插入件（15）的内表面上的芯上。 在树脂聚合并除去芯之前进行用含有树脂的组合物浸渍的纤维结构的成型。

公开(公告)号：US08715956B2

公开(公告)日：2014-05-06

申请号：US12597863

申请日：2008-04-22

Applicant: Jérémy Pruvost ,  Jack Legrand ,  Alain Foucault

Inventor： Jérémy Pruvost ,  Jack Legrand ,  Alain Foucault

IPC: C12P1/00

CPC classification number: C12P1/00 ,  B01D11/0288 ,  C12M21/02 ,  C12M29/18 ,  C12M47/06 ,  C12P23/00 ,  C12R1/89

Abstract: A method is provided for extracting cellular compounds from micro-organisms. The method includes culturing the micro-organisms and extracting the cellular compounds from the micro-organisms. Each step is carried out in a continuous manner, wherein the extraction step is carried out separately from the culture step and in conditions of biocompatibility with the micro-organisms. The method further includes at least one step of recovering the cellular compounds and at least one step of recirculating the micro-organisms towards the culture step.

Abstract translation: 提供了从微生物提取细胞化合物的方法。 该方法包括培养微生物并从微生物中提取细胞化合物。 每个步骤以连续的方式进行，其中提取步骤与培养步骤和与微生物的生物相容性的条件分开进行。 该方法还包括至少一个回收细胞化合物的步骤和将微生物再循环到培养步骤的至少一个步骤。

公开(公告)号：US08177933B2

公开(公告)日：2012-05-15

申请号：US12856081

申请日：2010-08-13

Applicant: Alain Foucault ,  Etienne Juchauld ,  David Marsal ,  Stéphane Otin

Inventor： Alain Foucault ,  Etienne Juchauld ,  David Marsal ,  Stéphane Otin

IPC: B29C53/56 ,  B65H81/00 ,  F04D29/32

CPC classification number: B29C70/68 ,  B29L2031/08 ,  F01D5/282 ,  F01D5/34 ,  F05D2300/44 ,  F05D2300/603 ,  F05D2300/614 ,  Y02T50/672

Abstract: A first insert (15) forming a ring is placed inside and in contact with a core (10) in degradable material. On the outer face of the core a plurality of inserts (20) is arranged each comprising a platform (21) applied against the outer face of the core and at least one blade (22) extending substantially radially outwards. A fibrous structure (56) is wound around the core passing over the platforms (21) and over the inner surface of the ring-shaped insert (15). Molding of the fibrous structure, impregnated with a composition containing a resin, is performed before polymerization of the resin and removal of the core.

Abstract translation: 形成环的第一插入件（15）放置在可降解材料的内部并与芯（10）接触。 在芯的外表面上，布置有多个插入件（20），每个插入件包括施加在芯的外表面上的平台（21）和基本上径向向外延伸的至少一个叶片（22）。 纤维结构（56）缠绕在穿过平台（21）并且在环形插入件（15）的内表面上的芯上。 在树脂聚合并除去芯之前进行用含有树脂的组合物浸渍的纤维结构的成型。

公开(公告)号：US20060243665A1

公开(公告)日：2006-11-02

申请号：US10562726

申请日：2004-06-28

Applicant: Francois Couillard ,  Alain Foucault ,  Daniel Durand

Inventor： Francois Couillard ,  Alain Foucault ,  Daniel Durand

IPC: B01D15/08

CPC classification number: G01N21/07 ,  B01D15/1892 ,  G01N30/42

Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (1) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Abstract translation: 本发明涉及一种通过液 - 液离心色谱分离液体进料的成分的方法和用于实施该液体进料的装置。 具有不同分配系数的成分（A，B），使得它们分别通过轻溶剂（1）和较重溶剂（L）以不同速度携带，将进料在分离塔（col）的中间点连续注入 ），分别在由串联的至少一组分离单元的互连构成的柱的两端分别进行包括重溶剂注入阶段和轻溶剂注入阶段的连续循环，分离的溶剂（FA， FB）在与溶剂注入点相对的列端部的每个阶段期间收集。

公开(公告)号：US20100178669A1

公开(公告)日：2010-07-15

申请号：US12597863

申请日：2008-04-22

Applicant: Jeremy Pruvost ,  Jack Legrand ,  Alain Foucault

Inventor： Jeremy Pruvost ,  Jack Legrand ,  Alain Foucault

IPC: C12P1/00 ,  C12M1/00

CPC classification number: C12P1/00 ,  B01D11/0288 ,  C12M21/02 ,  C12M29/18 ,  C12M47/06 ,  C12P23/00 ,  C12R1/89

Abstract: HA method is provided for extracting cellular compounds from micro-organisms. The method includes culturing the micro-organisms and extracting the cellular compounds from the micro-organisms. Each step is carried out in a continuous manner, wherein the extraction step is carried out separately from the culture step and in conditions of biocompatibility with the micro-organisms. The method further includes at least one step of recovering the cellular compounds and at least one step of recirculating the micro-organisms towards the culture step.

Abstract translation: 提供HA方法从微生物中提取细胞化合物。 该方法包括培养微生物并从微生物中提取细胞化合物。 每个步骤以连续的方式进行，其中提取步骤与培养步骤和与微生物的生物相容性的条件分开进行。 该方法还包括至少一个回收细胞化合物的步骤和将微生物再循环到培养步骤的至少一个步骤。

公开(公告)号：US06537452B1

公开(公告)日：2003-03-25

申请号：US09926246

申请日：2001-09-28

Applicant: François de La Poype ,  Roland De La Poype ,  Patrick Durand ,  Alain Foucault ,  Jack Legrand ,  Gérard Patissier ,  Jean-Michel Rosant

Inventor： François de La Poype ,  Roland De La Poype ,  Patrick Durand ,  Alain Foucault ,  Jack Legrand ,  Gérard Patissier ,  Jean-Michel Rosant

IPC: B01D1508

CPC classification number: B01D15/1892 ,  B01D15/22 ,  B01D15/30 ,  G01N30/42 ,  G01N30/6091

Abstract: A centrifuge partition chromatography device for separating a liquid into at least two phases, comprising at least one flat ring (3a) that can be rotationally driven around its axis, disposed in a substantially vertical position. Said ring (3a) comprises a plurality of cells (7) whose dimensions are greater in a first direction than the dimensions in a second direction which is substantially perpendicular to the first, disposed side by side and distributed along the periphery of said ring (3a) in addition to being connected to each other in a series by inlet and outlet ducts (15) terminating in the opposite respective ends of each cells (7) enabling the circulation of liquid. The invention is characterized in that said ducts (15) are disposed in such a way that the liquid supply jet penetrates the cells (7) in a direction that includes at least one radial component relative to the axis of rotation of said ring (3a).

Abstract translation: 一种用于将液体分离成至少两相的离心机分配色谱装置，包括至少一个围绕其轴旋转地驱动的平环（3a），所述平环设置在基本垂直的位置。 所述环（3a）包括多个单元（7），其尺寸在第一方向上大于沿着第二方向的尺寸，所述第二方向基本上垂直于第一方向并排布置，并且沿着所述环（3a）的周边分布 ）除了通过入口和出口管道（15）彼此连接之外，终止于每个电池单元（7）的相对的相应端部，使得能够循环液体。 本发明的特征在于，所述管道（15）以这样的方式设置，使得液体供应射流沿着包括相对于所述环（3a）的旋转轴线的至少一个径向分量的方向穿透细胞（7） 。

公开(公告)号：US07553421B2

公开(公告)日：2009-06-30

申请号：US10593774

申请日：2005-03-17

Applicant: Alain Foucault ,  Jack Legrand ,  Luc Marchal ,  Daniel Durand

Inventor： Alain Foucault ,  Jack Legrand ,  Luc Marchal ,  Daniel Durand

IPC: B01D15/08

CPC classification number: G01N30/42

Abstract: Method for sizing the cells of a centrifugal liquid-liquid chromatography “column” (CPC column) consisting of stacked discs on which the cells connected in cascade (in series) by small channels are engraved. Rotation of the stack creates a high centrifugal acceleration field which makes it possible to keep a liquid phase referred to as stationary phase motionless, whereas a mobile phase circulates along the CPC column. The cells are three-dimensional, with two dimensions (L, l) of the cells oriented in a plane substantially normal to the axis of rotation (Ω) of the disc and a third dimension (e) oriented in a direction substantially parallel to the axis of rotation, and selected so as to be at least equal to one of the other two dimensions (L, l), thus providing higher efficiency. When the scale of the devices has to be changed, the size of the cells is modified ensuring that, in any case, this third dimension (e) is favored so as to be as great as possible.Applications: design of analytic or preparative chromatography devices.

Abstract translation: 对由小型通道串联连接（串联）的单元的堆叠盘组成的离心液 - 液相色谱“柱”（CPC柱）的细胞尺寸调整方法。 堆的旋转产生高的离心加速场，这使得可以将液相保持为静止相，而流动相沿着CPC柱循环。 细胞是三维的，细胞的两个维度（L，1）定位在基本上垂直于盘的旋转轴线（Ω）的平面中，并且第三维度（e）在基本上平行于 并且被选择为至少等于其他两个维度（L，1）中的一个，从而提供更高的效率。 当必须改变设备的规模时，修改单元的大小，确保在任何情况下，第三维度（e）都是有利的，以便尽可能地大。 应用：分析或制备色谱装置的设计。

公开(公告)号：US07422685B2

公开(公告)日：2008-09-09

申请号：US10562726

申请日：2004-06-28

Applicant: François Couillard ,  Alain Foucault ,  Daniel Durand

Inventor： François Couillard ,  Alain Foucault ,  Daniel Durand

IPC: B01D15/08

CPC classification number: G01N21/07 ,  B01D15/1892 ,  G01N30/42

Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (1) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Abstract translation: 本发明涉及一种通过液 - 液离心色谱分离液体进料的成分的方法和用于实施该液体进料的装置。 具有不同分配系数的成分（A，B），使得它们分别通过轻溶剂（1）和较重溶剂（L）以不同速度携带，将进料在分离塔（col）的中间点连续注入 ），分别在由串联的至少一组分离单元的互连构成的柱的两端分别进行包括重溶剂注入阶段和轻溶剂注入阶段的连续循环，分离的溶剂（FA， FB）在与溶剂注入点相对的列端部的每个阶段期间收集。

公开(公告)号：US20080035546A1

公开(公告)日：2008-02-14

申请号：US10593774

申请日：2005-03-17

Applicant: Alain Foucault ,  Jack Legrand ,  Luc Marchal ,  Daniel Durand

Inventor： Alain Foucault ,  Jack Legrand ,  Luc Marchal ,  Daniel Durand

IPC: G01N30/42

CPC classification number: G01N30/42

Abstract: Method for sizing the cells of a centrifugal liquid-liquid chromatography “column” (CPC column) consisting of stacked discs on which the cells connected in cascade (in series) by small channels are engraved. Rotation of the stack creates a high centrifugal acceleration field which makes it possible to keep a liquid phase referred to as stationary phase motionless, whereas a mobile phase circulates along the CPC column. The cells are three-dimensional, with two dimensions (L, I) of the cells oriented in a plane substantially normal to the axis of rotation (Ω) of the disc and a third dimension (e) oriented in a direction substantially parallel to the axis of rotation, and selected so as to be at least equal to one of the other two dimensions (L, I), thus providing higher efficiency. When the scale of the devices has to be changed, the size of the cells is modified ensuring that, in any case, this third dimension (e) is favoured so as to be as great as possible.

Abstract translation: 对由小型通道串联连接（串联）的单元的堆叠盘组成的离心液 - 液相色谱“柱”（CPC柱）的细胞尺寸调整方法。 堆的旋转产生高的离心加速场，这使得可以将液相保持为静止相，而流动相沿着CPC柱循环。 细胞是三维的，其中细胞的两个维度（L，I）定向在基本上垂直于盘的旋转轴线（Ω）的平面中，并且第三维度（e）在基本上平行于 并且被选择为至少等于其他两个维度（L，I）中的一个，从而提供更高的效率。 当必须改变设备的规模时，修改单元的大小，确保在任何情况下，第三维度（e）都是有利的，以便尽可能地大。