公开(公告)号：US5646029A

公开(公告)日：1997-07-08

申请号：US276452

申请日：1994-07-18

申请人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

发明人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

IPC分类号： C12N15/09 ,  A23J1/00 ,  A61K38/00 ,  A61K47/48 ,  C07H21/04 ,  C07K14/415 ,  C07K16/16 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/91 ,  C12N1/21 ,  C12N5/00 ,  C12N5/06 ,  C12N5/14

CPC分类号： C07K14/415 ,  A23J1/007 ,  C12N15/8241 ,  A61K38/00

摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.

摘要翻译： 本发明提供植物阿拉伯半乳聚糖蛋白（AGP）及其基因。 从Nicotiana alata，Nicotiana plumbaginafolia和Pyrus communis分离AGPs。 提出了分离的AGP肽片段的氨基酸序列。 使用分离的AGP片段合成寡核苷酸探针以制备用于PCR的寡核苷酸引物或制备RNA探针以筛选N.alata，N.plumbaginafolia和P. communis的cDNA文库。 分离编码分离的AGP片段的氨基酸序列的cDNA克隆。 本发明首次呈现源自相应AGP基因的完整AGP氨基酸序列。 本发明还提供了可用于获得编码AGP肽的AGP基因的方法，所述AGP肽包含特异性分离的富含脯氨酸（富含OAST）（OAST）序列或特异性分离的不存在羟脯氨酸的序列。

公开(公告)号：US5830747A

公开(公告)日：1998-11-03

申请号：US798744

申请日：1997-02-13

申请人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

发明人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

IPC分类号： C12N15/09 ,  A23J1/00 ,  A61K38/00 ,  A61K47/48 ,  C07H21/04 ,  C07K14/415 ,  C07K16/16 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/91 ,  C12N1/20 ,  A01H1/04 ,  C12N15/00

CPC分类号： C07K14/415 ,  A23J1/007 ,  C12N15/8241 ,  A61K38/00

摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.

摘要翻译： 本发明提供植物阿拉伯半乳聚糖蛋白（AGP）及其基因。 从Nicotiana alata，Nicotiana plumbaginafolia和Pyrus communis分离AGPs。 提出了分离的AGP肽片段的氨基酸序列。 使用分离的AGP片段合成寡核苷酸探针以制备用于PCR的寡核苷酸引物或制备RNA探针以筛选N.alata，N.plumbaginafolia和P. communis的cDNA文库。 分离编码分离的AGP片段的氨基酸序列的cDNA克隆。 本发明首次呈现源自相应AGP基因的完整AGP氨基酸序列。 本发明还提供了可用于获得编码AGP肽的AGP基因的方法，所述AGP肽包含特异性分离的富含脯氨酸（富含OAST）（OAST）序列或特异性分离的不存在羟脯氨酸的序列。