公开(公告)号：US08067005B1

公开(公告)日：2011-11-29

申请号：US09719045

申请日：1999-06-08

申请人： Andrew Paul Chapman ,  David John King

发明人： Andrew Paul Chapman ,  David John King

IPC分类号： A61K39/395 ,  C07K16/00

CPC分类号： C07K16/2863 ,  A61K47/60 ,  C07K16/00 ,  C07K16/241 ,  C07K2317/55 ,  Y10S424/809 ,  Y10S530/866

摘要： Divalent antibody fragments are described, each of which has one or more interchain bridges containing a synthetic or naturally occurring polymer selected from a polyalkylene, polyakenylene, polyoxyalkylene or polysaccharide. Each bridge may be the residue of a homo- or heterobifunctional cross-linking reagent and serves to link two heavy chains in each antibody fragment via the sulphur atoms of cysteine residues present in the chains. Each fragment may be attached to one or more effector or reporter molecules, and is of use in therapy or diagnostics where it has markedly improved binding and/or pharmacokinetic properties when compared to other antibody fragments which have the same number and type of polymer molecules but in which the polymer molecules are randomly attached.

摘要翻译： 描述了二价抗体片段，其各自具有一个或多个含有选自聚亚烷基，聚亚乙基，聚氧化烯或多糖的合成或天然存在的聚合物的链间桥。 每个桥可以是同功异构或异双功能交联剂的残基，并且用于通过存在于链中的半胱氨酸残基的硫原子连接每个抗体片段中的两个重链。 每个片段可以连接到一个或多个效应物或报道分子上，并且在治疗或诊断中有用，其中与具有相同数量和类型的聚合物分子的其它抗体片段相比，其具有显着改善的结合和/或药代动力学性质，但是 其中聚合物分子是随机连接的。

公开(公告)号：US07977464B2

公开(公告)日：2011-07-12

申请号：US12141667

申请日：2008-06-18

申请人： Diljeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

发明人： Diljeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

IPC分类号： C12P21/08 ,  C07K16/00 ,  A61K39/395 ,  C12P21/04 ,  C07H21/04

CPC分类号： C07K16/241 ,  A61K47/6845 ,  A61K47/6875 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565

摘要： There is disclosed antibody molecules containing at least one CDR derived from a mouse monoclonal antibody having specificity for human TNFα. There is also disclosed a CDR grafted antibody wherein at least one of the CDRs is a hybrid CDR. Further disclosed are DNA sequences encoding the chains of the antibody molecules, vectors, transformed host cells and uses of the antibody molecules in the treatment of diseases mediated by TNFα.

摘要翻译： 公开了含有至少一种衍生自对人TNFα具有特异性的小鼠单克隆抗体的CDR的抗体分子。 还公开了CDR移植抗体，其中至少一个CDR是杂交CDR。 还公开了编码抗体分子链，载体，转化的宿主细胞的链DNA序列以及抗体分子在治疗TNFα介导的疾病中的用途。

公开(公告)号：US07662609B2

公开(公告)日：2010-02-16

申请号：US10531402

申请日：2003-10-15

申请人： David Paul Humphreys ,  Andrew Paul Chapman ,  Martyn Kim Robinson ,  Mariangela Spitali

发明人： David Paul Humphreys ,  Andrew Paul Chapman ,  Martyn Kim Robinson ,  Mariangela Spitali

IPC分类号： C12N1/21 ,  C12N15/13

CPC分类号： C07K14/245 ,  C12N1/20

摘要： The present invention provides an Ecoli host cell expressing a recombinant antibody characterized in that the Ecoli host cell has been genetically modified in order to change at least one physical property of one or more Ecoli proteins which in the wild type copurify with said recombinant antibody.

摘要翻译： 本发明提供了表达重组抗体的大肠杆菌宿主细胞，其特征在于已将遗传修饰的大肠杆菌宿主细胞改变为野生型与所述重组抗体共培养的一种或多种大肠杆菌蛋白质的至少一种物理性质。

公开(公告)号：US20080269465A1

公开(公告)日：2008-10-30

申请号：US12141667

申请日：2008-06-18

申请人： DILJEET SINGH ATHWAL ,  DEREK THOMAS BROWN ,  ANDREW NEIL CHARLES WEIR ,  ANDREW GEORGE POPPLEWELL ,  ANDREW PAUL CHAPMAN ,  DAVID JOHN KING

发明人： DILJEET SINGH ATHWAL ,  DEREK THOMAS BROWN ,  ANDREW NEIL CHARLES WEIR ,  ANDREW GEORGE POPPLEWELL ,  ANDREW PAUL CHAPMAN ,  DAVID JOHN KING

IPC分类号： C07K16/24

CPC分类号： C07K16/241 ,  A61K47/6845 ,  A61K47/6875 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565

摘要： There is disclosed antibody molecules containing at least one CDR derived from a mouse monoclonal antibody having specificity for human TNFα. There is also disclosed a CDR grafted antibody wherein at least one of the CDRs is a hybrid CDR. Further disclosed are DNA sequences encoding the chains of the antibody molecules, vectors, transformed host cells and uses of the antibody molecules in the treatment of diseases mediated by TNFα.

摘要翻译： 公开了含有至少一种衍生自对人TNFα具有特异性的小鼠单克隆抗体的CDR的抗体分子。 还公开了CDR移植抗体，其中至少一个CDR是杂交CDR。 进一步公开的是编码抗体分子链，载体，转化宿主细胞的链DNA序列和抗体分子在治疗由TNFα介导的疾病中的用途。

公开(公告)号：US07186820B2

公开(公告)日：2007-03-06

申请号：US09949559

申请日：2001-09-10

申请人： Diljeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

发明人： Diljeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

IPC分类号： C07H21/04 ,  C12P21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/08 ,  C07K16/00 ,  A61K39/395 ,  C12N15/02

CPC分类号： C07K16/241 ,  A61K47/6845 ,  A61K47/6875 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565

摘要： There is disclosed antibody molecules containing at least one CDR derived from a mouse monoclonal antibody having specificity for human TNFα. There is also disclosed a CDR grafted antibody wherein at least one of the CDRs is a hybrid CDR. Further disclosed are DNA sequences encoding the chains of the antibody molecules, vectors, transformed host cells and uses of the antibody molecules in the treatment of diseases mediated by TNFα.

摘要翻译： 公开了含有至少一种衍生自对人TNFα具有特异性的小鼠单克隆抗体的CDR的抗体分子。 还公开了CDR移植抗体，其中至少一个CDR是杂交CDR。 进一步公开的是编码抗体分子链，载体，转化宿主细胞的链DNA序列和抗体分子在治疗由TNFα介导的疾病中的用途。

公开(公告)号：US07012135B2

公开(公告)日：2006-03-14

申请号：US09875221

申请日：2001-06-06

申请人： Diljeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

发明人： Diljeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

IPC分类号： C07K16/24

CPC分类号： C07K16/241 ,  A61K47/6845 ,  A61K47/6875 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565

摘要： There is disclosed antibody molecules containing at least one CDR derived from a mouse monoclonal antibody having specificity for human TNFα. There is also disclosed a CDR grafted antibody wherein at least one of the CDRs is a hybrid CDR. Further disclosed are DNA sequences encoding the chains of the antibody molecules, vectors, transformed host cells and uses of the antibody molecules in the treatment of diseases mediated by TNFα.

公开(公告)号：US20100285530A1

公开(公告)日：2010-11-11

申请号：US12645064

申请日：2009-12-22

申请人： David Paul Humphreys ,  Andrew Paul Chapman ,  Martyn Kim Robinson ,  Mariangela Spitali

发明人： David Paul Humphreys ,  Andrew Paul Chapman ,  Martyn Kim Robinson ,  Mariangela Spitali

IPC分类号： C12P21/08 ,  C12N1/21

CPC分类号： C07K14/245 ,  C12N1/20

摘要： The present invention provides an E. coli host cell expressing a recombinant antibody characterized in that the E. coli host cell has been genetically modified in order to change at least one physical property of one or more E. coli proteins which in the wild type co-purify with said recombinant antibody.

摘要翻译： 本发明提供了表达重组抗体的大肠杆菌宿主细胞，其特征在于大肠杆菌宿主细胞已被遗传修饰以改变一种或多种大肠杆菌蛋白质的至少一种物理性质，其在野生型共 用所述重组抗体纯化。

公开(公告)号：US07402662B2

公开(公告)日：2008-07-22

申请号：US11374231

申请日：2006-03-13

申请人： Dilijeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

发明人： Dilijeet Singh Athwal ,  Derek Thomas Brown ,  Andrew Neil Charles Weir ,  Andrew George Popplewell ,  Andrew Paul Chapman ,  David John King

IPC分类号： C12P21/08 ,  C07K16/00 ,  A61K39/395 ,  C12P21/04 ,  C07H21/04

CPC分类号： C07K16/241 ,  A61K47/6845 ,  A61K47/6875 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565

摘要： There is disclosed antibody molecules containing at least one CDR derived from a mouse monoclonal antibody having specificity for human TNFα. There is also disclosed a CDR grafted antibody wherein at least one of the CDRs is a hybrid CDR. Further disclosed are DNA sequences encoding the chains of the antibody molecules, vectors, transformed host cells and uses of the antibody molecules in the treatment of diseases mediated by TNFα.

摘要翻译： 公开了含有至少一种衍生自对人TNFα具有特异性的小鼠单克隆抗体的CDR的抗体分子。 还公开了CDR移植抗体，其中至少一个CDR是杂交CDR。 进一步公开的是编码抗体分子链，载体，转化宿主细胞的链DNA序列和抗体分子在治疗由TNFα介导的疾病中的用途。