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    Transposable element-anchored, amplification method for isolation and identification of tagged genes
    5.
    发明授权
    Transposable element-anchored, amplification method for isolation and identification of tagged genes 失效
    可转移元件锚定，用于分离和鉴定标记基因的扩增方法

    公开(公告)号：US06881539B1

    公开(公告)日：2005-04-19

    申请号：US09622353

    申请日：1999-02-16

    申请人： John A. Arbuckle ,  Timothy W. Fox ,  Robert B. Meeley ,  Benjamin A. Bowen ,  John A. McElver

    发明人： John A. Arbuckle ,  Timothy W. Fox ,  Robert B. Meeley ,  Benjamin A. Bowen ,  John A. McElver

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1065 ,  C12N15/1079 ,  C12N15/1082

    摘要： A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.

    摘要翻译： 提供了用于快速分离和鉴定转座元件标记基因的DNA序列的方法。 该方法包括使用可转座元件锚定扩增来鉴定和克隆与突变表型相关的扩增产物的修饰AFLP方法。 一旦被克隆，可以使用目的扩增产物直接筛选cDNA文库或进行测序，并与序列同源性的可用数据库进行比较。 该方法的修改提供了用于将额外类型的插入事件的位置识别到基因组DNA中的方法，更具体地说是将基因插入到宿主生物体的基因组中。

    Compositions and methods for locating preferred integration sites within a plant genome
    8.
    发明授权
    Compositions and methods for locating preferred integration sites within a plant genome 有权
    用于定位植物基因组内优选整合位点的组合物和方法

    公开(公告)号：US06552248B1

    公开(公告)日：2003-04-22

    申请号：US09438239

    申请日：1999-11-12

    申请人： Christopher L. Baszczynski ,  Benjamin A. Bowen ,  David J. Peterson ,  Laura A. Tagliani

    发明人： Christopher L. Baszczynski ,  Benjamin A. Bowen ,  David J. Peterson ,  Laura A. Tagliani

    IPC分类号： A01H500

    CPC分类号： C12N15/8216 ,  C07K2319/00 ,  C12N9/00 ,  C12N15/102 ,  C12N15/1137 ,  C12N15/8203 ,  C12N15/8205 ,  C12N15/8213 ,  C12N15/8274 ,  C12N15/90 ,  C12N2310/321 ,  C12N2310/53 ,  C12N2310/3521

    摘要： Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites, Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene, By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.

    摘要翻译： 提供了在植物基因组内找到最佳整合位点的方法。 更具体地，用具有表达盒的靶位点转化植物，所述表达盒包含可操作地连接于在植物中有活性的启动子的核苷酸序列。 靶位点侧翼为不相同的重组位点，可以测试转化的原生质体，组织或整个植物以确定插入的基因的活性水平。通过比较不同插入位点中基因的细胞活性，优选的整合位点 可以发现其中基因以高或可接受的水平表达。 然后可以利用这些植物与随后的重定靶技术将核苷酸序列替换为包含在转移盒中的其它感兴趣的基因或核苷酸序列。