公开(公告)号：US20090017061A1

公开(公告)日：2009-01-15

申请号：US11795508

申请日：2006-01-18

Applicant: Bernard Jan Appelmelk ,  Wilhelmus Bitter ,  Peter Andre van der Ley

Inventor： Bernard Jan Appelmelk ,  Wilhelmus Bitter ,  Peter Andre van der Ley

IPC: A61K39/04 ,  C12N9/10 ,  C07K14/00 ,  C07H21/00 ,  C12N1/21 ,  A61P37/04 ,  C12N1/20 ,  C12N15/74 ,  A61K35/74 ,  G01N33/53

CPC classification number: C12N9/1051 ,  A61K2039/522

Abstract: The present invention relates to mycobacterial lipoarabinomannan cap-specific mannosyl transferases and nucleic acid encoding such transferases. The invention further relates to Mycobacteria in which the lipoarabinomannan cap-specific mannosyl transferases have been inactivated and that therefore express mannose cap-deficient lipoarabinomannan. Such Mycobacteria with mannose cap-deficient lipoarabinomannan may be used as more effective vaccines against mycobacterial diseases as they lack the immunosuppressive action of the mannose cap.

Abstract translation: 本发明涉及分枝杆菌脂褐素甘露聚糖帽特异性甘露糖基转移酶和编码这种转移酶的核酸。 本发明还涉及分子细菌，其中脂肪阿拉伯甘露聚糖帽特异性甘露糖基转移酶已被灭活，因此表达甘露糖帽缺乏型脂肪阿拉伯聚糖。 这种具有甘露糖帽缺乏型脂肪阿拉伯聚糖的分枝杆菌可能被用作更有效的针对分枝杆菌疾病的疫苗，因为它们缺乏甘露糖帽的免疫抑制作用。

公开(公告)号：US06624140B1

公开(公告)日：2003-09-23

申请号：US09493211

申请日：2000-01-28

Applicant: Philip Richard Abraham ,  Bernard Jan Appelmelk ,  Sander Jan Hendrik Van Deventer

Inventor： Philip Richard Abraham ,  Bernard Jan Appelmelk ,  Sander Jan Hendrik Van Deventer

IPC: A01N3718

CPC classification number: G01N33/579 ,  A61K38/00 ,  C07K14/4723 ,  C07K14/4742 ,  Y02A50/411 ,  Y02A50/58

Abstract: A peptide with an amino acid composition such that the peptide is amphipathic, cationic and forms a stable &agr;-helix and has the following structure comprising at least 12 amino acids R1-R2-A1-B1-(A2-B2-C1-A3)m-(C2)n-R3, wherein A=an amino acid selected from the basic amino acids Lys,Arg or His B=an amino acid selected from the aromatic amino acids Phe, Trp or Tyr C=an amino acid selected from the group comprising the hydrophobic amino acids Leu, Ile, Val or Ala, and said peptide has either the orientation according to the formula or the retro orientation thereof, wherein at least 0-m of the repetitive sequence motifs (A2-B2-C1-A3) have the retro orientation and the remaining repetitive motifs (A2-B2-C1-A3) have the orientation as presented in the formula and wherein, R1-R2- and R3 are a number of amino acids, and wherein m=1-10, preferably 2-8, more preferably 2-5 and n=1-3, a pharmaceutical composition comprising such a peptide application thereof in treatment or diagnosis related to i.a. parasite infection topical and systemic tumors and septic shock.

Abstract translation: 具有氨基酸组成的肽，使得肽是两亲性的，阳离子的并形成稳定的α-螺旋，并且具有包含至少12个氨基酸的以下结构A =选自碱性氨基酸Lys，Arg或HisB =的氨基酸 氨基酸选自芳族氨基酸Phe，Trp或TyrC =选自疏水性氨基酸Leu，Ile，Val或Ala的氨基酸，所述肽具有根据式的取向或其复古方向， 其中至少0-m的重复序列基序（A2-B2-C1-A3）具有反向取向，并且其余的重复基序（A2-B2-C1-A3）具有如式中所示的取向，并且其中R1 -R2-和R3是多个氨基酸，并且其中m = 1-10，优选2-8，更优选2-5和n = 1-3，药物组合物包含其在治疗或诊断中的这种肽应用 ia 寄生虫感染局部和全身肿瘤和败血性休克。