公开(公告)号：US4835098A

公开(公告)日：1989-05-30

申请号：US772561

申请日：1985-09-04

Applicant: Harry T. Orr ,  Beverly H. Koller

Inventor： Harry T. Orr ,  Beverly H. Koller

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/172 ,  Y10S436/808

Abstract: HLA class I and class II locus specific DNA probes are prepared by cloning DNA segments obtained from the 3'-untranslated region and the 5' flanking region of isolated alleles. The probes are useful to determine the HLA alleles present in an individual.

Abstract translation: HLA I类和II类基因座特异性DNA探针通过克隆从3'非翻译区和5'侧翼区分离的等位基因获得的DNA片段来制备。 探针可用于确定个体中存在的HLA等位基因。

公开(公告)号：US06677501B2

公开(公告)日：2004-01-13

申请号：US09875318

申请日：2001-06-06

Applicant: Christopher A. Gabel ,  Beverly H. Koller

Inventor： Christopher A. Gabel ,  Beverly H. Koller

IPC: A61K67027

CPC classification number: C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2217/20 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0312 ,  A01K2267/0325 ,  A01K2267/0368 ,  C07K14/705

Abstract: The invention provides non-human, genetically-modified mammals and genetically modified animals cells having a functionally disrupted P2×7 receptor gene. Also provided are methods for producing genetically modified mice in which one or both P2×7R alleles have been functionally inactivated.

Abstract translation: 本发明提供了非人类，遗传修饰的哺乳动物和具有功能性破坏的P2x7受体基因的遗传修饰的动物细胞。 还提供了用于生产基因修饰小鼠的方法，其中一个或两个P2x7R等位基因已被功能失活。

公开(公告)号：US5574205A

公开(公告)日：1996-11-12

申请号：US175469

申请日：1993-12-30

Applicant: Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies ,  Robert B. Dubridge ,  Gary Greenburg ,  Daniel J. Capon ,  Steven R. Williams ,  Mariona L. A. De Rafael

Inventor： Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies ,  Robert B. Dubridge ,  Gary Greenburg ,  Daniel J. Capon ,  Steven R. Williams ,  Mariona L. A. De Rafael

IPC: A01K67/027 ,  A61K35/12 ,  A61K38/21 ,  A61K45/00 ,  C07H21/04 ,  C07K14/74 ,  C12N5/02 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/85 ,  C12Q1/68 ,  G01N33/53 ,  A61K48/00 ,  A61K49/00 ,  C12N5/00

CPC classification number: A01K67/0271 ,  A01K67/0275 ,  A01K67/0276 ,  C07K14/70539 ,  C12N15/00 ,  C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/025 ,  A01K2267/03 ,  A01K2267/0381

Abstract: Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, each of the .beta..sub.2- microglobulin gene and the IFN-.gamma.R gene is inactivated for reducing or eliminating the expression of functional MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.

Abstract translation: 同源重组用于灭活基因，特别是与MHC抗原相关的基因。 特别地，每个β2-微球蛋白基因和IFN-γR基因被灭活以减少或消除功能性MHC抗原的表达。 所得细胞可用作通用供体细胞。 此外，胚胎干细胞可以通过同源重组来修饰，用于产生嵌合或转基因哺乳动物宿主，其可以用作通用供体器官的来源，或作为药物和移植疗法的模型。 还描述了在非转化的哺乳动物体细胞中同源重组的方法。

公开(公告)号：US5416260A

公开(公告)日：1995-05-16

申请号：US611020

申请日：1990-11-09

Applicant: Beverly H. Koller ,  Oliver Smithies

Inventor： Beverly H. Koller ,  Oliver Smithies

IPC: A01K67/027 ,  C07H21/04 ,  C07K14/74 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/85 ,  C12N5/00

CPC classification number: C12N15/8509 ,  A01K67/0271 ,  A01K67/0276 ,  C07K14/70539 ,  C12N15/00 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/025 ,  A01K2267/03

Abstract: Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs.

Abstract translation: 同源重组用于灭活基因，特别是与MHC抗原相关的基因。 特别地，β2-微球蛋白基因被灭活用于减少或消除I类MHC抗原。 所得细胞可用作通用供体。 此外，胚胎干细胞可以通过同源重组来修饰，用于产生嵌合或转基因哺乳动物宿主，其可以用作通用供体器官的来源。

公开(公告)号：US5413923A

公开(公告)日：1995-05-09

申请号：US990879

申请日：1992-12-11

Applicant: Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies

Inventor： Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies

IPC: A01K67/027 ,  C12N15/00 ,  C12N15/85 ,  C12N5/00

CPC classification number: C12N15/8509 ,  A01K67/0271 ,  A01K67/0276 ,  C12N15/00 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03

Abstract: Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies.

Abstract translation: 同源重组用于灭活基因，特别是与MHC抗原相关的基因。 特别地，β2-微球蛋白基因被灭活用于减少或消除I类MHC抗原。 所得细胞可用作通用供体。 此外，胚胎干细胞可以通过同源重组来修饰，用于产生嵌合或转基因哺乳动物宿主，其可以用作通用供体器官的来源，或作为药物和移植疗法的模型。

公开(公告)号：US06514752B1

公开(公告)日：2003-02-04

申请号：US08443613

申请日：1995-05-18

Applicant: Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies ,  Robert B. Dubridge ,  Gary Greenburg ,  Daniel J. Capon ,  Steven R. Williams ,  Mariona Lourdes Arbones De Rafael

Inventor： Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies ,  Robert B. Dubridge ,  Gary Greenburg ,  Daniel J. Capon ,  Steven R. Williams ,  Mariona Lourdes Arbones De Rafael

IPC: C12N1574

CPC classification number: A01K67/0271 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/025 ,  A01K2267/03 ,  A01K2267/0381 ,  C07K14/70539 ,  C12N15/00 ,  C12N15/8509

Abstract: Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the &bgr;2-microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.

Abstract translation: 同源重组用于灭活基因，特别是与MHC抗原相关的基因。 特别地，β2-微球蛋白基因被灭活以减少或消除功能性I类MHC抗原的表达。 所得细胞可用作通用供体细胞。 此外，胚胎干细胞可以通过同源重组来修饰，用于产生嵌合或转基因哺乳动物宿主，其可以用作通用供体器官的来源，或作为药物和移植疗法的模型。 还描述了在非转化的哺乳动物体细胞中同源重组的方法。

公开(公告)号：US6139835A

公开(公告)日：2000-10-31

申请号：US443647

申请日：1995-05-18

Applicant: Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies ,  Robert B. Dubridge ,  Gary Greenburg ,  Daniel J. Capon ,  Steven R. Williams ,  Mariona Lourdes Arbones De Rafael

Inventor： Raju Kucherlapati ,  Beverly H. Koller ,  Oliver Smithies ,  Robert B. Dubridge ,  Gary Greenburg ,  Daniel J. Capon ,  Steven R. Williams ,  Mariona Lourdes Arbones De Rafael

IPC: A01K67/027 ,  A61K35/12 ,  A61K38/21 ,  A61K45/00 ,  C07H21/04 ,  C07K14/74 ,  C12N5/02 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/85 ,  C12Q1/68 ,  G01N33/53 ,  C12N15/63

CPC classification number: A01K67/0271 ,  A01K67/0275 ,  A01K67/0276 ,  C07K14/70539 ,  C12N15/00 ,  C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/025 ,  A01K2267/03 ,  A01K2267/0381

Abstract: Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2- microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as allogeneic donor cells. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.

Abstract translation: 同源重组用于灭活基因，特别是与MHC抗原相关的基因。 特别地，β2-微球蛋白基因被灭活以减少或消除功能性I类MHC抗原的表达。 所得细胞可用作同种异体供体细胞。 还描述了在非转化的哺乳动物体细胞中同源重组的方法。