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    Sequential Method
    1.
    发明申请
    Sequential Method 审中-公开
    顺序方法

    公开(公告)号：US20090215196A1

    公开(公告)日：2009-08-27

    申请号：US11572316

    申请日：2005-07-15

    Applicant: Bo Ek ,  Mats Holmquist ,  Karolina Osterlund ,  Mats Inganas

    Inventor： Bo Ek ,  Mats Holmquist ,  Karolina Osterlund ,  Mats Inganas

    IPC: G01N33/566

    CPC classification number: G01N33/54306 ,  B01L3/5027 ,  G01N33/54366

    Abstract: A method for determination of an analyte in an original liquid sample by performing an inhibition affinity assay in a microchannel structure of a microfluidic device. The main characteristic feature is the steps of: (i) providing the microfluidic device in a form where the microchannel structure comprises a reaction microcavity which a) an inlet end, and b) contains a solid phase with binding sites (BS) for an analyte or an analyte-related entity, (ii) providing a liquid sample (1) containing the analyte or the analyte-related entity within the microchannel structure at the inlet end of the reaction microcavity, and flowing sample (1) through the reaction microcavity under conditions such that the analyte or the analyte-related entity is captured by BS leaving a portion of BS unoccupied (free BS), (iii) providing a liquid sample (2) which contains an analytically detectable analogue of the analyte or of the analyte-related entity, and flowing sample (2) through said reaction microcavity to capture the analogue by free BS, and (iv) measuring the amount of analyte analogue captured in step (iii) and relating this amount to the amount of analyte in an original sample.

    Abstract translation: 一种通过在微流体装置的微通道结构中进行抑制亲和力测定来测定原始液体样品中的分析物的方法。 主要特征是以下步骤：（i）以微通道结构包含反应微腔的形式提供微流体装置，其中a）入口端和b）包含具有用于分析物的结合位点（BS）的固相 或分析物相关实体，（ii）在反应微腔的入口端处提供在微通道结构内含有分析物或分析物相关实体的液体样品（1），并将样品（1）流过反应微腔下 条件，使得分析物或分析物相关实体被BS捕获，留下一部分BS未占用（自由BS），（iii）提供含有分析物或分析物 - 分析物的可分析检测的类似物的液体样品（2） 相关实体和流动样品（2）通过所述反应微腔以通过游离BS捕获类似物，和（iv）测量步骤（iii）中捕获的分析物类似物的量，并将该量与在 原始样本

    Starch branching enzyme II of potato
    2.
    发明授权
    Starch branching enzyme II of potato 失效
    土豆淀粉分支酶Ⅱ

    公开(公告)号：US06469231B1

    公开(公告)日：2002-10-22

    申请号：US09658499

    申请日：2000-09-08

    Applicant: Bo Ek ,  Jamshid Khosnoodi ,  Clas-Tomas Larsson ,  Håkan Larsson ,  Lars Rask

    Inventor： Bo Ek ,  Jamshid Khosnoodi ,  Clas-Tomas Larsson ,  Håkan Larsson ,  Lars Rask

    IPC: C12N1582

    CPC classification number: C12N9/107 ,  C12N15/8245

    Abstract: The present invention relates to an amino acid sequence of second starch branching enzyme (SBE II) of potato and a fragment thereof as well as to the corresponding isolated DNA sequences. Furthermore, the invention relates to vectors comprising such an isolated DNA sequence, to processes for production of transgenic potatoes, and to the use of said potatoes for the production of starch. The starch obtained will show a changed pattern of branching of amylopectin as well as a changed amylose/amylopectin ratio.

    Abstract translation: 本发明涉及马铃薯及其片段的第二淀粉分支酶（SBE II）的氨基酸序列以及相应的分离的DNA序列。 此外，本发明涉及包含这种分离的DNA序列的载体，用于生产转基因马铃薯的方法以及所述马铃薯用于生产淀粉的用途。 获得的淀粉将显示支链淀粉分支的变化模式以及直链淀粉/支链淀粉比例的变化。

    Starch branching enzyme II of potato
    3.
    发明授权
    Starch branching enzyme II of potato 失效
    土豆淀粉分支酶Ⅱ

    公开(公告)号：US06169226A

    公开(公告)日：2001-01-02

    申请号：US09087277

    申请日：1998-05-29

    Applicant: Bo Ek ,  Jamshid Khosnoodi ,  Clas-Tomas Larsson ,  Håkan Larsson ,  Lars Rask

    Inventor： Bo Ek ,  Jamshid Khosnoodi ,  Clas-Tomas Larsson ,  Håkan Larsson ,  Lars Rask

    IPC: C12N1529

    CPC classification number: C12N9/107 ,  C12N15/8245

    Abstract: The present invention relates to an amino acid sequence of second starch branching enzyme (SBE II) of potato and a fragment thereof as well as to the corresponding isolated DNA sequences. Furthermore, the invention relates to vectors comprising such an isolated DNA sequence, to processes for production of transgenic potatoes, and to the use of said potatoes for the production of starch. The starch obtained will show a changed pattern of branching of amylopectin as well as a changed amylose/amylopectin ratio.

    Abstract translation: 本发明涉及马铃薯及其片段的第二淀粉分支酶（SBE II）的氨基酸序列以及相应的分离的DNA序列。 此外，本发明涉及包含这种分离的DNA序列的载体，用于生产转基因马铃薯的方法，以及所述马铃薯用于生产淀粉的用途。 获得的淀粉将显示支链淀粉分支的变化模式以及直链淀粉/支链淀粉比例的变化。

    Filter sector for rotarydisc filters
    6.
    发明申请
    Filter sector for rotarydisc filters 审中-公开
    过滤器用于旋转式过滤器

    公开(公告)号：US20050082217A1

    公开(公告)日：2005-04-21

    申请号：US10499358

    申请日：2002-12-19

    Applicant: Conny Hagg ,  Bo Ek

    Inventor： Conny Hagg ,  Bo Ek

    IPC: B01D33/21 ,  B01D33/23 ,  B01D35/12

    CPC classification number: B01D33/23

    Abstract: A filter sector (1) for rotary disc filters comprises two outer walls (4, 5) of filter cloth defining a fitrate chamber (15) for receiving filtrate that has flowed through the outer walls. The outer walls of filter cloth are pleated to form a plurality of ridges and valleys as seen from outside the filter sector. Two liquid permeable support walls (11, 12) that solely support the valleys of the two outer walls (4, 5) of filter cloth, respectively, are arranged in the filtrate chamber and define a filtrate channel (14) with a filtrate outlet (3).

    Abstract translation: 用于旋转盘式过滤器的过滤器扇区（1）包括限定用于接收已经流过外壁的滤液的配合室（15）的两个滤布外壁（4,5）。 滤布的外壁被打褶以形成从过滤器部分的外部看到的多个脊和谷。 仅分别支撑滤布的两个外壁（4,5）的谷部的两个液体可渗透的支撑壁（11,12）分别布置在滤液室中，并且限定了具有滤液出口的滤液通道（14） 3）。

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