公开(公告)号：US20150182682A1

公开(公告)日：2015-07-02

申请号：US14140978

申请日：2013-12-26

申请人： Christopher Wegener ,  Bret Olson ,  Kyungyoon Min

发明人： Christopher Wegener ,  Bret Olson ,  Kyungyoon Min

IPC分类号： A61M1/34

CPC分类号： A61M1/3496 ,  A61M1/26 ,  A61M1/262 ,  A61M1/265 ,  A61M1/3633 ,  A61M2205/3365 ,  B01D63/16 ,  B01D2315/02

摘要： A method is provided for separating a suspension of cellular material comprising at least two differently-sized cell types using a spinning membrane separator. The method comprises selecting the cell type to be separated by passing through the membrane; determining a concentration of the selected cell type in the suspension; selecting an inlet flow rate for the suspension; selecting a rotational speed for the spinning membrane separator related to one or more of the concentration and relative size of the selected cell type in the suspension; rotating the spinning membrane separator at the selected rotational speed so that the selected cell type tends to migrate to regions of the shear field adjacent the porous membrane; and flowing the suspension through the spinning membrane separator.

摘要翻译： 提供了一种使用纺丝膜分离器分离包含至少两种不同尺寸的细胞类型的细胞材料的悬浮液的方法。 该方法包括通过穿过膜选择待分离的细胞类型; 确定悬浮液中所选细胞类型的浓度; 选择悬浮液的入口流量; 选择与悬浮液中所选择的细胞类型的浓度和相对大小中的一种或多种相关的纺丝膜分离器的旋转速度; 以选定的转速旋转纺丝膜分离器，使得所选择的细胞类型倾向于迁移到邻近多孔膜的剪切场的区域; 并使悬浮液流过纺丝膜分离器。