Molecular typing of listeria monocytogenes, hybridization supports and kits for said molecular typing
    1.
    发明申请
    Molecular typing of listeria monocytogenes, hybridization supports and kits for said molecular typing 审中-公开
    单核细胞增多性李斯特氏菌的分子分型,用于所述分子分型的杂交支持物和试剂盒

    公开(公告)号:US20060257894A1

    公开(公告)日:2006-11-16

    申请号:US11357421

    申请日:2006-02-21

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C12Q1/689 C12Q2600/16

    摘要: This invention provides 163 genes that are unique to the L. monocytogenes serovar 4b strain, CLIP80459, compared with L. monocytogenes EGDe strain. These genes are the basis for isolated nucleic acids, hybridization supports, and kits comprising a nucleotide sequence that is unique to a serovar 4b strain of L. monocytogenes. The invention also provides methods for identifying a L. monocytogenes in a sample, methods for identifying the lineage of a L. monocytogenes in a sample, and methods for sub-lineage typing of a L. monocytogenes in a sample. The invention also provides hybridization supports comprising a L. monocytogenes lineage-specific nucleic acid sequence, hybridization supports comprising a L. monocytogenes sub-lineage-specific nucleic acid sequence combination, and kits comprising a nucleic acid or hybridization support of the invention. Additionally, the invention provides specific macroarrays, methods, and kits for use in serotyping L. monocytogenes.

    摘要翻译: 本发明提供了与单核细胞增生李斯特氏菌EGDe株相比,独特于单核细胞增生李斯特氏菌血清型4b菌株CLIP80459的163种基因。 这些基因是分离的核酸,杂交载体和包含对单核细胞增生李斯特氏菌血清型4b菌株特有的核苷酸序列的试剂盒的基础。 本发明还提供了用于鉴定样品中单核细胞增生李斯特菌的方法,用于鉴定样品中单核细胞增生李斯氏菌谱系的方法,以及样品中单核细胞增生李斯氏菌的亚谱系分型方法。 本发明还提供了包含单核细胞增生李斯特菌谱系特异性核酸序列的杂交支持物,包含单核细胞增生李斯特氏菌属谱系特异性核酸序列组合的杂交支持体和包含本发明的核酸或杂交支持物的试剂盒。 另外,本发明提供用于血清型单核细胞增生李斯特菌的特异性大阵列,方法和试剂盒。