公开(公告)号：US09261496B2

公开(公告)日：2016-02-16

申请号：US13876293

申请日：2011-09-29

申请人： Roger Dale Kamm ,  Haruhiko Harry Asada ,  Waleed Ahmed Farahat ,  Ioannis K. Zervantonakis ,  Levi B. Wood ,  Chandrasekhar Kothapalli ,  Seok Chung ,  Jeffrey D. Macklis ,  Suzanne Tharin ,  Johanna Varner ,  Young Kum Park ,  Kwang Ho Lee ,  Le Thanh Tu Nguyen ,  Choong Kim

发明人： Roger Dale Kamm ,  Haruhiko Harry Asada ,  Waleed Ahmed Farahat ,  Ioannis K. Zervantonakis ,  Levi B. Wood ,  Chandrasekhar Kothapalli ,  Seok Chung ,  Jeffrey D. Macklis ,  Suzanne Tharin ,  Johanna Varner ,  Young Kum Park ,  Kwang Ho Lee ,  Le Thanh Tu Nguyen ,  Choong Kim

IPC分类号： C12M1/34 ,  C12M3/00 ,  G01N33/50 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/12

CPC分类号： G01N33/5008 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0816 ,  C12M23/16 ,  C12M23/22 ,  C12M25/14 ,  C12M29/04 ,  G01N33/5011 ,  G01N33/5029 ,  G01N33/5058 ,  G01N33/5064

摘要： Provided herein are microfluidic devices that can be used as a 3D bioassay, e.g., for drug screening, personalized medicine, tissue engineering, wound healing, and other applications. The device has a series of channels {e.g., small fluid channels) in a small polymer block wherein one or more of the channels can be filled with a biologically relevant gel, such as collagen, which is held in place by posts. As shown herein, when the device is plated with cells such as endothelial cells, new blood vessels grow in the gel, which is thick enough for the cells to grow in three dimensions. Other channels, e.g., fluid channels, allow drugs or biological material to be exposed to the 3D cell growth. Cells, such as endothelial cells, can be cultured and observed as they grow on the surface of a 3D gel scaffold, where e.g., rates of angiogenesis can be measured, as well as intervascularization and extravascularization of cancerous cells.

摘要翻译： 本文提供的微流体装置可用作3D生物测定，例如用于药物筛选，个性化医学，组织工程，伤口愈合和其它应用。 该装置在小聚合物嵌段中具有一系列通道（例如，小的流体通道），其中一个或多个通道可以填充生物相关凝胶，例如通过柱保持在适当位置的胶原。 如本文所示，当该装置镀有诸如内皮细胞的细胞时，新的血管在凝胶中生长，其足够厚以使细胞在三维中生长。 其他通道，例如流体通道，允许药物或生物材料暴露于3D细胞生长。 可以在3D凝胶支架的表面上生长细胞，例如内皮细胞，并且观察细胞，其中例如可以测量血管发生的速率，以及癌细胞的血管间血管形成和血管外再造。

公开(公告)号：US08529951B1

公开(公告)日：2013-09-10

申请号：US12034237

申请日：2008-02-20

申请人： Anand Ramamurthi ,  Chandrasekhar Kothapalli ,  Binata Joddar

发明人： Anand Ramamurthi ,  Chandrasekhar Kothapalli ,  Binata Joddar

IPC分类号： A61K38/18

CPC分类号： A61K38/1841 ,  A61K38/30 ,  A61L31/042 ,  A61L31/16 ,  A61L2300/102 ,  A61K2300/00 ,  C08L5/08

摘要： Disclosed are elastogenic cues that can be utilized to encourage growth and development of elastin-containing cellular constructs. The elastogenic cues include hyaluronan fragments and oligomers, optionally in conjunction with growth factors and/or a source of copper ions. The elastogenic cues can up-regulate elastin matrix synthesis and by vascular smooth muscle cells. In addition to encouraging synthesis of elastin in a cellular matrix and organization into elastic fibers, the elastogenic cues can also stabilize the formed ECM matrix through suppression of elastin-laminin receptor (ELR). In addition, the elastogenic cues can inhibit cell hyper-proliferation (e.g., hyperplasia) common in inflammatory vascular disease.

摘要翻译： 公开了可用于促进含弹性蛋白的细胞构建体的生长和发育的弹性成因提示。 弹性成分提示包括透明质酸片段和寡聚物，任选地与生长因子和/或铜离子源结合。 弹性蛋白提示可以上调弹性蛋白基质合成和血管平滑肌细胞。 除了鼓励将细胞基质和组织中的弹性蛋白合成为弹性纤维之外，弹性成分提示还可以通过抑制弹性蛋白 - 层粘连蛋白受体（ELR）来稳定形成的ECM基质。 此外，弹性引发线索可以抑制炎性血管疾病中常见的细胞增生（例如，增生）。

公开(公告)号：US20140057311A1

公开(公告)日：2014-02-27

申请号：US13876293

申请日：2011-09-29

申请人： Roger Dale Kamm ,  Haruhiko Harry Asada ,  Waleed Ahmed Farahat ,  Ioannis K. Zervantonakis ,  Levi B. Wood ,  Chandrasekhar Kothapalli ,  Seok Chung ,  Jeffrey D. Macklis ,  Suzanne Tharin ,  Johanna Varner ,  Young Kum Park ,  Kwang Ho Lee ,  Le Thanh Tu Nguyen ,  Choong Kim

发明人： Roger Dale Kamm ,  Haruhiko Harry Asada ,  Waleed Ahmed Farahat ,  Ioannis K. Zervantonakis ,  Levi B. Wood ,  Chandrasekhar Kothapalli ,  Seok Chung ,  Jeffrey D. Macklis ,  Suzanne Tharin ,  Johanna Varner ,  Young Kum Park ,  Kwang Ho Lee ,  Le Thanh Tu Nguyen ,  Choong Kim

IPC分类号： G01N33/50

CPC分类号： G01N33/5008 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0816 ,  C12M23/16 ,  C12M23/22 ,  C12M25/14 ,  C12M29/04 ,  G01N33/5011 ,  G01N33/5029 ,  G01N33/5058 ,  G01N33/5064

摘要： Provided herein are microfluidic devices that can be used as a 3D bioassay, e.g., for drug screening, personalized medicine, tissue engineering, wound healing, and other applications. The device has a series of channels {e.g., small fluid channels) in a small polymer block wherein one or more of the channels can be filled with a biologically relevant gel, such as collagen, which is held in place by posts. As shown herein, when the device is plated with cells such as endothelial cells, new blood vessels grow in the gel, which is thick enough for the cells to grow in three dimensions. Other channels, e.g., fluid channels, allow drugs or biological material to be exposed to the 3D cell growth. Cells, such as endothelial cells, can be cultured and observed as they grow on the surface of a 3D gel scaffold, where e.g., rates of angiogenesis can be measured, as well as intervascularization and extravascularization of cancerous cells.

摘要翻译： 本文提供的微流体装置可用作3D生物测定，例如用于药物筛选，个性化医学，组织工程，伤口愈合和其它应用。 该装置在小聚合物嵌段中具有一系列通道（例如，小的流体通道），其中一个或多个通道可以填充生物相关凝胶，例如通过柱保持在适当位置的胶原。 如本文所示，当装置镀有诸如内皮细胞的细胞时，新的血管在凝胶中生长，其足够厚以使细胞在三维中生长。 其他通道，例如流体通道，允许药物或生物材料暴露于3D细胞生长。 可以在3D凝胶支架的表面上生长细胞，例如内皮细胞，并且观察细胞，其中例如可以测量血管发生的速率，以及癌细胞的血管间血管形成和血管外再造。