Beta-glucosidase enzymes
    1.
    发明授权
    Beta-glucosidase enzymes 有权
    β-葡糖苷酶

    公开(公告)号:US08486683B2

    公开(公告)日:2013-07-16

    申请号:US12790079

    申请日:2010-05-28

    摘要: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.

    摘要翻译: 提供衍生自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶,其在一个或多个位置43,101,260和/或其中包含氨基酸取代的一个或多个动力学参数(KG,KG2,kcat)表现出改善, 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体,用于从宿主菌株产生修饰的β-葡糖苷酶的方法,以及在纤维素水解中使用经修饰的β-葡糖苷酶。

    Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes
    2.
    发明授权
    Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes 有权
    使用辅酶进行木质纤维素原料的酶水解

    公开(公告)号:US08017361B2

    公开(公告)日:2011-09-13

    申请号:US12201374

    申请日:2008-08-29

    摘要: Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.

    摘要翻译: 提供了用于将预处理的木质纤维素原料水解成可溶性糖的酶混合物。 酶混合物包含约0.25至约0.83(w / w)的分数浓度(fEG4)的EG4,分数浓度(fSwo1)为约0至约0.66(w / w)的溶血素,和分数浓度的Cip1 (fCip1)相对于酶混合物中存在的所有辅助酶测量为0至约0.33。 还提供了使用酶混合物将预处理的木质纤维素原料转化为可溶性糖的方法,以及使用和生产这种酶混合物的方法。

    NOVEL BETA-GLUCOSIDASE ENZYMES
    3.
    发明申请
    NOVEL BETA-GLUCOSIDASE ENZYMES 有权
    新型β-葡萄糖苷酶

    公开(公告)号:US20100304438A1

    公开(公告)日:2010-12-02

    申请号:US12790079

    申请日:2010-05-28

    摘要: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.

    摘要翻译: 提供衍生自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶,其在一个或多个位置43,101,260和/或其中包含氨基酸取代的一个或多个动力学参数(KG,KG2,kcat)表现出改善, 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体,用于从宿主菌株产生修饰的β-葡糖苷酶的方法,以及在纤维素水解中使用经修饰的β-葡糖苷酶。

    ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC FEEDSTOCKS USING ACCESSORY ENZYMES
    9.
    发明申请
    ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC FEEDSTOCKS USING ACCESSORY ENZYMES 有权
    使用附件酶的木质纤维素饲料的酶水解

    公开(公告)号:US20090061484A1

    公开(公告)日:2009-03-05

    申请号:US12201374

    申请日:2008-08-29

    IPC分类号: C12P21/04 C12P19/14 C12N9/42

    摘要: Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.

    摘要翻译: 提供了用于将预处理的木质纤维素原料水解成可溶性糖的酶混合物。 酶混合物包含约0.25至约0.83(w / w)的分数浓度(fEG4)的EG4,分数浓度(fSwo1)为约0至约0.66(w / w)的溶血素,和分数浓度的Cip1 (fCip1)相对于酶混合物中存在的所有辅助酶测量为0至约0.33。 还提供了使用酶混合物将预处理的木质纤维素原料转化为可溶性糖的方法,以及使用和生产这种酶混合物的方法。