-
公开(公告)号:US20200299638A1
公开(公告)日:2020-09-24
申请号:US16823916
申请日:2020-03-19
摘要: Provided is a liquid set for a droplet discharging apparatus, the liquid set including: Liquid A containing a multi-branched polymer A, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more electrophilic functional groups in at least one of a side chain(s) and an end(s); and Liquid B containing a multi-branched polymer B, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more nucleophilic functional groups in at least one of a side chain(s) and an end(s); the Liquid A and the Liquid B each having a pH of from 5 to 10, and containing the multi-branched polymer at a concentration of from 0.3% by mass to 20% by mass.
-
2.
公开(公告)号:US20210154932A1
公开(公告)日:2021-05-27
申请号:US16950133
申请日:2020-11-17
申请人: Tatsuya SAMESHIMA , Hidekazu YAGINUMA , Takahiko MATSUMOTO , Momoko SHIONOIRI , Natsuko IWASHITA , Chihiro KUBO , Naoki SATOH , Takehiro YAMAZAKI
发明人: Tatsuya SAMESHIMA , Hidekazu YAGINUMA , Takahiko MATSUMOTO , Momoko SHIONOIRI , Natsuko IWASHITA , Chihiro KUBO , Naoki SATOH , Takehiro YAMAZAKI
IPC分类号: B29C64/209 , B33Y30/00 , B33Y70/00
摘要: A liquid discharge head including: a film member including a discharge hole configured to discharge liquid; and a displacement member configured to displace a position of the film member to discharge the liquid from the discharge hole, wherein an edge of the film member forming the discharge hole has a curve in a cross section of the film member in a thickness direction of the film member, and a surface of the film member includes a passive film.
-
公开(公告)号:US20190309257A1
公开(公告)日:2019-10-10
申请号:US16375490
申请日:2019-04-04
申请人: Tomoyuki Aratani , Chihiro Kubo , Hidekazu Yaginuma , Naoki Satoh
发明人: Tomoyuki Aratani , Chihiro Kubo , Hidekazu Yaginuma , Naoki Satoh
摘要: Provided is a method for producing a cell locating plate, including: a step of laminating a structural layer over a plane of a base material, the structural layer being a layer that is formed of a cell non-adhesive material and in which a plurality of holes are formed; a step of coating a coating liquid in the holes and over the plane of the base material exposed through the holes; and a step of discharging a cell suspension onto the plane coated with the coating liquid.
-
公开(公告)号:US20170267975A1
公开(公告)日:2017-09-21
申请号:US15459146
申请日:2017-03-15
申请人: Aino Hasegawa , Daisuke Takagi , Shigeo Hatada , Yuuma Usui , Chihiro Kubo , Shusaku Shiomoto , Waka Lin
发明人: Aino Hasegawa , Daisuke Takagi , Shigeo Hatada , Yuuma Usui , Chihiro Kubo , Shusaku Shiomoto , Waka Lin
IPC分类号: C12N5/077
CPC分类号: C12N5/0656 , C12N5/0062 , C12N2533/54 , C12N2533/74
摘要: Provided is a three-dimensional culture structure that is excellent in cell adhesion and cell stretching and can be produced efficiently. The three-dimensional culture structure includes cells, a cell support material configured to support the cells, and bioaffinity particles. In a preferable mode, the bioaffinity particles are exposed from at least part of the surface of the cell support material, or the bioaffinity particles are protruded from the cell support material, or a surface area occupation rate at which the bioaffinity particles are exposed is 20% or greater of the entire surface of the three-dimensional culture structure, or the bioaffinity particles are dispersed in the cell support material.
-
公开(公告)号:US20210147795A1
公开(公告)日:2021-05-20
申请号:US17094935
申请日:2020-11-11
申请人: Natsuko IWASHITA , Hidekazu YAGINUMA , Momoko SHIONOIRI , Tatsuya SAMESHIMA , Chihiro KUBO , Naoki SATOH , Takehiro YAMAZAKI
发明人: Natsuko IWASHITA , Hidekazu YAGINUMA , Momoko SHIONOIRI , Tatsuya SAMESHIMA , Chihiro KUBO , Naoki SATOH , Takehiro YAMAZAKI
摘要: An object of the present invention is to provide a layered body of cells that is used in a co-culture technique and that is capable of recognizing as paracrine effect of each cell and detecting the effect with high intensity. The layered body of the present invention is a layered body 1A having a layered structure in which a gel layer 20a containing a hydrogel is disposed between at least two cell layers 10a and 10b containing cells of different types from each other, wherein the hydrogel is a multi-branched polymer hydrogel formed by a reaction of: Liquid A containing a multi-branched polymer A, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more electrophilic functional groups in at least one of a side chain(s) and an end(s); and Liquid B containing a multi-branched polymer B, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more nucleophilic functional groups in at least one of a side chain(s) and an end(s), the concentration of components derived from the multi-branched polymers A and B in the hydrogel is from 0.6 to 8% by weight, and the thickness is from 0.02 mm to 2 mm.
-
6.
公开(公告)号:US20190284524A1
公开(公告)日:2019-09-19
申请号:US16299873
申请日:2019-03-12
申请人: Hidekazu YAGINUMA , Naoki SATOH , Tomoyuki ARATANI , Chihiro KUBO , Daisuke TAKAGI , Momoko SHIONOIRI , Natsuko IWASHITA
发明人: Hidekazu YAGINUMA , Naoki SATOH , Tomoyuki ARATANI , Chihiro KUBO , Daisuke TAKAGI , Momoko SHIONOIRI , Natsuko IWASHITA
摘要: Provided is a cell tissue producing method including: a cell adhesive portion forming step of forming a cell adhesive portion formed of a cell adhesive material and having a predetermined shape at a predetermined position over a substrate having a cell non-adhesive surface; and a cell locating step of discharging a cell suspension containing at least cells and a cell drying inhibitor onto the cell adhesive portion in the form of a liquid droplet to locate the cells.
-
-
-
-
-