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公开(公告)号:US07455990B2
公开(公告)日:2008-11-25
申请号:US10693234
申请日:2003-10-24
申请人: Claus Lindvald Johansen , Søren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
发明人: Claus Lindvald Johansen , Søren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
摘要: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
摘要翻译: 描述了一种用于释放可溶性或膜相关胞内细胞内蛋白质(POI)的方法,包括以下步骤:提供包含可溶性或膜相关细胞内POI的细胞; 使细胞与膜提取组合物接触; 并且使得POI在足以特异性释放POI并且以可溶形式的条件下从细胞释放。
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公开(公告)号:US20090098592A1
公开(公告)日:2009-04-16
申请号:US12243364
申请日:2008-10-01
申请人: Claus Lindvald JOHANSEN , Soren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
发明人: Claus Lindvald JOHANSEN , Soren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
摘要: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
摘要翻译: 描述了一种用于释放可溶性或膜相关胞内细胞内蛋白质(POI)的方法,包括以下步骤:提供包含可溶性或膜结合的细胞内POI的细胞; 使细胞与膜提取组合物接触; 并且使得POI在足以特异性释放POI并且以可溶形式的条件下从细胞释放。
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公开(公告)号:US20120083426A1
公开(公告)日:2012-04-05
申请号:US13188031
申请日:2011-07-21
申请人: Claus Lindvald JOHANSEN , Soren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
发明人: Claus Lindvald JOHANSEN , Soren Kjaerulff , Susan Mampusta Madrid , Henrik Pedersen , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi
IPC分类号: C40B30/08 , G01N33/566 , C12N9/88 , C09K3/00 , G01N33/573 , C07K14/00 , C12Q1/26 , C12Q1/02 , C07K1/14 , C12N9/04
摘要: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
摘要翻译: 描述了一种用于释放可溶性或膜相关胞内细胞内蛋白质(POI)的方法,包括以下步骤:提供包含可溶性或膜相关细胞内POI的细胞; 使细胞与膜提取组合物接触; 并且使得POI在足以特异性释放POI并且以可溶形式的条件下从细胞释放。
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