公开(公告)号：US20060162002A1

公开(公告)日：2006-07-20

申请号：US11282262

申请日：2005-11-18

Applicant: Cynthia Kenyon ,  Javier Apfeld ,  Andrew Dillin ,  Delia Garigan ,  Ao-Lin Hsu ,  Josh Lehrer-Graiwer ,  Coleen Murphy

Inventor： Cynthia Kenyon ,  Javier Apfeld ,  Andrew Dillin ,  Delia Garigan ,  Ao-Lin Hsu ,  Josh Lehrer-Graiwer ,  Coleen Murphy

IPC: A01K67/033 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C12N15/111 ,  A01K67/0336 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/43545 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2330/31 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/527 ,  C12Q1/533 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2500/10

Abstract: The present invention relates to regulation of adult lifespan in eukaryotes. More particularly, the present invention is directed to methods of assaying for genes, gene products, and genes in pathways controlled by such genes and gene products, using RNAi and microarray analysis, that regulate lifespan (e.g., extend or truncate adult lifespan) in eukaryotes such as invertebrates (e.g., C. elegans), plants, and mammals, e.g., humans. For example, the present invention is directed to genes encoding components of the mitochondrial respiratory chain and genes encoding glycolysis enzymes, which are involved in lifespan regulation, and genes and gene products in pathways controlled by such genes. Other genes and gene products identified as regulating aging and aging pathways include a gene encoding a GTPase; a transcriptional activator; novel genes: llw-1, llw-2, llw-3, and llw-4; genes encoding cytochrome P450 proteins (involved in steroid biosynthesis); a melatonin synthesis gene; genes encoding insulin and insulin-like peptides; genes encoding heat shock factors; genes encoding catalases; stress-response genes; and metabolic genes. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, antisense nucleic acids, and ribozymes, that regulate, e.g., enhance, adult lifespan via modulation of aging associated proteins; as well as to the use of expression profiles, markers, and compositions in diagnosis and therapy related to lifespan extension, life expectancy, and aging. The present invention also relates to gene therapy involving lifespan associated genes.

公开(公告)号：US07794957B2

公开(公告)日：2010-09-14

申请号：US11282262

申请日：2005-11-18

Applicant: Cynthia Kenyon ,  Javier Apfeld ,  Andrew Dillin ,  Delia Garigan ,  Ao-Lin A. Hsu ,  Josh Lehrer-Graiwer ,  Coleen Murphy

Inventor： Cynthia Kenyon ,  Javier Apfeld ,  Andrew Dillin ,  Delia Garigan ,  Ao-Lin A. Hsu ,  Josh Lehrer-Graiwer ,  Coleen Murphy

IPC: G01N33/567 ,  G01N33/53 ,  C12N5/00 ,  A01N61/00 ,  C07K1/00

CPC classification number: C12N15/111 ,  A01K67/0336 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/43545 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2330/31 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/527 ,  C12Q1/533 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2500/10

Abstract: The present invention relates to regulation of adult lifespan in eukaryotes. More particularly, the present invention is directed to methods of assaying for activators of the heat shock factor 1 (HSF-1) protein, which increases lifespan when overexpressed in an organism.

Abstract translation: 本发明涉及真核生物中成人寿命的调节。 更具体地，本发明涉及测定热休克因子1（HSF-1）蛋白的激活剂的方法，其增加在生物体中过表达时的寿命。

公开(公告)号：US20060228707A1

公开(公告)日：2006-10-12

申请号：US10536635

申请日：2003-08-11

Applicant: Cynthia Kenyon ,  Jennifer Berman ,  Andrew Dillin ,  Malene Hansen ,  Ao-lin Hsu ,  Coleen Murphy

Inventor： Cynthia Kenyon ,  Jennifer Berman ,  Andrew Dillin ,  Malene Hansen ,  Ao-lin Hsu ,  Coleen Murphy

IPC: C12Q1/68 ,  C12Q1/26 ,  A01K67/033 ,  C40B40/08

CPC classification number: A01K67/0338 ,  A01K67/0336 ,  A01K2217/058 ,  A01K2227/703 ,  A01K2267/03 ,  C12Q1/26 ,  G01N33/5085 ,  G01N33/6893

Abstract: The present invention relates to regulation of lifespan in eukaryotes. More particularly, one aspect of the present invention is the identification of genes, gene products, and genes in pathways controlled by such genes and gene products, using RNAi and microarray analysis, that regulate lifespan (e.g., extend or truncate adult lifespan) in eukaryotes such as invertebrates (e.g., C. elegans), plants, and mammals, e.g., humans. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, antisense nucleic acids, and ribozymes, that regulate, e.g., enhance, adult lifespan via modulation of aging associated proteins; as well as to the use of expression profiles, promoters, reporter genes, markers, and compositions in diagnosis and therapy related to lifespan extension, life expectancy, and aging. The present invention also relates to gene therapy involving lifespan associated genes.

Abstract translation: 本发明涉及真核生物的寿命调节。 更具体地，本发明的一个方面是使用RNAi和微阵列分析来鉴定由这些基因和基因产物控制的途径中的基因，基因产物和基因，其调节真核生物的寿命（例如延长或截短成年寿命） 例如无脊椎动物（例如秀丽隐杆线虫），植物和哺乳动物，例如人类。 本发明还涉及用于鉴定和使用包括小分子化学组成，抗体，反义核酸和核酶在内的试剂的方法，所述试剂通过老化相关蛋白的调节来调控例如增强成年寿命; 以及在与寿命延长，预期寿命和衰老相关的诊断和治疗中使用表达谱，启动子，报道基因，标记物和组合物。 本发明还涉及涉及寿命相关基因的基因治疗。