利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.019 秒)

    METHODS FOR PREPARING SINGLE DOMAIN ANTIBODY MICROARRAYS
    5.
    发明申请
    METHODS FOR PREPARING SINGLE DOMAIN ANTIBODY MICROARRAYS 审中-公开
    用于制备单一抗体微阵列的方法

    公开(公告)号：US20140235492A1

    公开(公告)日：2014-08-21

    申请号：US14345660

    申请日：2011-09-20

    申请人： Daniel Baty ,  Patrick Chames ,  Klervi Even-Desrumeaux

    发明人： Daniel Baty ,  Patrick Chames ,  Klervi Even-Desrumeaux

    IPC分类号： G01N33/531 ,  G01N33/543

    CPC分类号： G01N33/531 ,  C07K16/1045 ,  C07K16/18 ,  C07K16/3007 ,  C07K16/3015 ,  C07K16/32 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/90 ,  G01N33/54353

    摘要： The present invention relates to a method for preparing sd Ab microarray comprising the step consisting of: —i) providing a host cell capable of expressing a biotinylation enzyme —ii) transforming said host cell with a nucleic acid encoding for a fusion protein wherein a single domain antibody is fused at its carboxy terminal end to a biotinylation peptide —iii) culturing said host cell in presence of biotin in such a way that said fusion protein and biotinylation enzyme are expressed, resulting in biotinylation of said fusion protein —iv) lysing said host cell as cultured at step iii) —v) spotting the lysate obtained at step iv) on a solid sup port coated with an agent selected from the group consisting of avidin, streptavidin and/or any art known derivative of these agents A further object of the invention relates to a sd Ab microarray obtainable by the method of the invention.

    摘要翻译： 本发明涉及一种制备sd Ab微阵列的方法，包括以下步骤：-i）提供能够表达生物素化酶的宿主细胞-ii）用编码融合蛋白的核酸转化所述宿主细胞，其中单个 结构域抗体在其羧基末端融合到生物素化肽-iii），以生物素存在下培养所述宿主细胞，使得所述融合蛋白和生物素化酶被表达，导致所述融合蛋白的生物素化-iv）裂解所述 在步骤iii）中培养的宿主细胞-v）将在步骤iv）获得的裂解物点染色在涂有选自抗生物素蛋白，链霉亲和素和/或任何本领域已知的这些试剂衍生物的试剂的固体支持物上。另一个目的 本发明涉及通过本发明的方法可获得的sd Ab微阵列。