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公开(公告)号:US20080009078A1
公开(公告)日:2008-01-10
申请号:US11725769
申请日:2007-03-19
申请人: Roger O'Neill , Marc Glazer , Tom Yang , Daniel Suich , Karl Voss
发明人: Roger O'Neill , Marc Glazer , Tom Yang , Daniel Suich , Karl Voss
IPC分类号: G01N33/566 , B05D3/00 , G01N33/00
CPC分类号: G01N33/54366 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , G01N27/44726 , G01N27/44795 , G01N33/582
摘要: Methods and apparatus are provided to resolve analytes within a fluid path using isoelectric focusing, gel electrophoresis, or other separation means. Materials within the fluid path that are compatible with these separation means are used to attach resolved analytes to the wall of the fluid path. Attachment results from a triggerable event such as photoactivation, thermal activation, or chemical activation. In accordance with a further aspect of the present invention, the material in the capillary may also be disrupted, by either the triggerable event or a subsequent event such as melting or photocleavage. Thus, an open lumen or porous structure may be created within the fluid path, allowing unbound analyte materials to be washed from the fluid path, and detection agents to be washed into the fluid path. The separation-compatible materials may be polymerizable monomers, gels, entangled polymers or other materials.
摘要翻译: 提供了使用等电聚焦,凝胶电泳或其他分离方法来解决流体路径内的分析物的方法和装置。 使用与这些分离装置相容的流体路径内的材料将分解的分析物附着到流体路径的壁上。 附件来自可触发事件,如光活化,热激活或化学活化。 根据本发明的另一方面,毛细管中的材料也可以通过可触发事件或随后的事件(例如熔化或光切割)而被破坏。 因此,可以在流体路径内产生开放的内腔或多孔结构,允许从流体路径洗涤未结合的分析物质,以及要洗涤到流体路径中的检测剂。 分离相容材料可以是可聚合单体,凝胶,缠结聚合物或其它材料。
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公开(公告)号:US20070062813A1
公开(公告)日:2007-03-22
申请号:US11524630
申请日:2006-09-20
申请人: Erik Gentalen , Daniel Suich
发明人: Erik Gentalen , Daniel Suich
CPC分类号: G01N27/44726 , C07K1/13 , C07K1/26 , C07K1/28 , C07K7/06 , C07K7/08 , G01N2550/00
摘要: Electrophoresis Compositions, methods and kits useful for, among other things, detecting, quantifying and/or characterizing analytes are provided. The compositions are useful as electrophoresis standards for determine the isoelectric point and molecular weight of an analyte. The electrophoresis standards generally comprise at least one label moiety and one or more reactive moieties that when activated attach the standard to a substrate.
摘要翻译: 提供了用于检测,量化和/或表征分析物的组合物,方法和试剂盒。 该组合物可用作用于测定分析物的等电点和分子量的电泳标准品。 电泳标准品通常包含至少一个标记部分和一个或多个反应性部分,当活化时将标准物附着到基底上。
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公开(公告)号:US20060128033A1
公开(公告)日:2006-06-15
申请号:US11343886
申请日:2006-01-30
申请人: Daniel Suich , Ronald Zuckermann
发明人: Daniel Suich , Ronald Zuckermann
IPC分类号: C40B40/02 , C40B40/10 , C07D311/82
CPC分类号: C07D493/10 , C09B11/24 , C09B49/126 , Y10T436/173845 , Y10T436/182
摘要: Fluorogenic or chromogenic dyes are useful as reporter molecules for detecting cell entry by a specific molecule.
摘要翻译: 荧光或显色染料可用作用于检测特定分子进入细胞的报道分子。
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