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公开(公告)号:US20070062813A1
公开(公告)日:2007-03-22
申请号:US11524630
申请日:2006-09-20
申请人: Erik Gentalen , Daniel Suich
发明人: Erik Gentalen , Daniel Suich
CPC分类号: G01N27/44726 , C07K1/13 , C07K1/26 , C07K1/28 , C07K7/06 , C07K7/08 , G01N2550/00
摘要: Electrophoresis Compositions, methods and kits useful for, among other things, detecting, quantifying and/or characterizing analytes are provided. The compositions are useful as electrophoresis standards for determine the isoelectric point and molecular weight of an analyte. The electrophoresis standards generally comprise at least one label moiety and one or more reactive moieties that when activated attach the standard to a substrate.
摘要翻译: 提供了用于检测,量化和/或表征分析物的组合物,方法和试剂盒。 该组合物可用作用于测定分析物的等电点和分子量的电泳标准品。 电泳标准品通常包含至少一个标记部分和一个或多个反应性部分,当活化时将标准物附着到基底上。
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公开(公告)号:US08586312B2
公开(公告)日:2013-11-19
申请号:US12858237
申请日:2010-08-17
申请人: Erik Gentalen , Mark Chee
发明人: Erik Gentalen , Mark Chee
CPC分类号: C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provides methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.
摘要翻译: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针,第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合,而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析,序列分析和表达监测的方法。
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公开(公告)号:US20050170412A1
公开(公告)日:2005-08-04
申请号:US11053164
申请日:2005-02-07
申请人: Erik Gentalen , Mark Chee
发明人: Erik Gentalen , Mark Chee
IPC分类号: G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , G01N33/566 , G01N37/00
CPC分类号: C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.
摘要翻译: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针,第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合,而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析,序列分析和表达监测的方法。
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公开(公告)号:US06852490B2
公开(公告)日:2005-02-08
申请号:US09930536
申请日:2001-08-14
申请人: Erik Gentalen , Mark Chee
发明人: Erik Gentalen , Mark Chee
IPC分类号: G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N33/566 , G01N37/00 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号: C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.
摘要翻译: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针,第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合,而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析,序列分析和表达监测的方法。
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公开(公告)号:US08945361B2
公开(公告)日:2015-02-03
申请号:US11524630
申请日:2006-09-20
申请人: Erik Gentalen , Daniel J. Suich
发明人: Erik Gentalen , Daniel J. Suich
CPC分类号: G01N27/44726 , C07K1/13 , C07K1/26 , C07K1/28 , C07K7/06 , C07K7/08 , G01N2550/00
摘要: Electrophoresis Compositions, methods and kits useful for, among other things, detecting, quantifying and/or characterizing analytes are provided. The compositions are useful as electrophoresis standards for determine the isoelectric point and molecular weight of an analyte. The electrophoresis standards generally comprise at least one label moiety and one or more reactive moieties that when activated attach the standard to a substrate.
摘要翻译: 提供了用于检测,量化和/或表征分析物的组合物,方法和试剂盒。 该组合物可用作用于测定分析物的等电点和分子量的电泳标准品。 电泳标准品通常包含至少一个标记部分和一个或多个反应性部分,当活化时将标准物附着到基底上。
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公开(公告)号:US20050191675A1
公开(公告)日:2005-09-01
申请号:US11053640
申请日:2005-02-07
申请人: Erik Gentalen , Mark Chee
发明人: Erik Gentalen , Mark Chee
IPC分类号: G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , G01N33/566 , G01N37/00
CPC分类号: C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.
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公开(公告)号:US06306643B1
公开(公告)日:2001-10-23
申请号:US09138958
申请日:1998-08-24
申请人: Erik Gentalen , Mark Chee
发明人: Erik Gentalen , Mark Chee
IPC分类号: C12M134
CPC分类号: C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.
摘要翻译: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针,第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合,而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析,序列分析和表达监测的方法。
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8.
公开(公告)号:US20100041569A1
公开(公告)日:2010-02-18
申请号:US11876920
申请日:2007-10-23
申请人: Erik Gentalen , Mark Chee
发明人: Erik Gentalen , Mark Chee
IPC分类号: C40B40/06
CPC分类号: C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.
摘要翻译: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针,第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合,而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析,序列分析和表达监测的方法。
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公开(公告)号:US20050064465A1
公开(公告)日:2005-03-24
申请号:US10884170
申请日:2004-07-02
申请人: Roger Dettloff , Celeste Kirby , Erik Gentalen , Monica Rosoff
发明人: Roger Dettloff , Celeste Kirby , Erik Gentalen , Monica Rosoff
CPC分类号: C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2400/0415 , B01L2400/049 , C12Q1/6844 , C12Q1/6865 , C12Q2565/629
摘要: Methods and systems for performing continuous amplification of RNA and other nucleic acids are provided. Expression profiling using the continuous flow RNA amplification systems are also provided.
摘要翻译: 提供了用于进行RNA和其他核酸的连续扩增的方法和系统。 还提供了使用连续流RNA扩增系统的表达谱。
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